LOCUS       Exported                 847 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    mEGFP-HRas S17N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(300..551)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(342..363)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cnnnatggng gagtcctcaa agacttggtg ttgttgatgg caaacacaca caggaagccc
       61 tccccggtgc gcatgtactg gtcccgcatg gcgctgtact cctcctggcc ggcggtatcc
      121 aggatgtcca acaggcacgt ctccccatca atgaccacct gcttccggta ggaatcctct
      181 atagtggggt cgtattcgtc cacaaaatgg ttctggatca gctggatggt cagcgcattc
      241 ttgcccacac cgccggcgcc caccaccacc agcttatatt ccgtcatctc gagtccggac
      301 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      361 atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg
      421 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      481 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      541 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      601 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      661 gtgtcgccct cgaacttcac ctcggcgcgg gtgttgtagt tgccgtcgtc cttgaaaaaa
      721 tggtgcgctc ctggactanc ntcnggcatg gcgganntaa naantctgct gctncatgtg
      781 ntcnggtang ctgaanactg cacccctant caggggtcnn angtggcagg acggcantnc
      841 tnggtna
//
