LOCUS Exported 2149 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).. ACCESSION . VERSION . KEYWORDS T1m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2149) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2149) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2149 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 37..54 /label=L4440 /note="L4440 vector, forward primer" misc_feature 60..80 /label=BioBrick suffix /note="universal suffix for all parts" misc_feature 151..172 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 175..218 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(222..240) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 308..412 /gene="bla" /label=AmpR promoter CDS 413..1273 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(631..650) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1444..2032 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1933..1952 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc 61 tgcagcggcc gctactagta ggtctctaag ctctcaagga cagaaaagcc cgcctttcgg 121 cgggctttga gtaccttgag acccactagt ctctagaagc ggccgcgaat tccagaaatc 181 atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga tacgcctatt 241 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 421 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 481 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 541 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 601 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 661 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 721 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 781 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 841 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 901 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 961 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 1021 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 1081 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt cgcgcggtat 1141 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 1201 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 1261 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 1321 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 1381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 1441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 1501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 1561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 1621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 1681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 1741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 1801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 1861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 1921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 1981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga aaaacgccag 2041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 2101 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtga //