LOCUS Exported 12878 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants. ACCESSION . VERSION . KEYWORDS R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12878) AUTHORS Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC TITLE Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. JOURNAL Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. PUBMED 31535972 REFERENCE 2 (bases 1 to 12878) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12878 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 213..235 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 227..244 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 228..244 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 269..289 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 2308..2332 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2346..2387 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 2580..3350 /codon_start=1 /product="N-terminally truncated mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=miniTurbo /translation="IQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACI AEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAIGLSLVIGIVMAEVLRKLGADK VRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK" CDS 3357..3383 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" CDS 3414..4133 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3459..3480) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3720..3739) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4067..4088 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(4156..4180) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 4212..4464 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(4497..4513) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4497..4513) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4510..4532) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4521..4537 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4545..4575) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4590..4611 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." terminator 4647..4899 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(5059..5610) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(5662..5841) /label=NOS promoter /note="nopaline synthase promoter" misc_feature 6347..6371 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 6892..7683 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 7929..8517 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8418..8437 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8671..8688 /label=L4440 /note="L4440 vector, forward primer" misc_feature 8703..8843 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(8769..8788) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8929..8951 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 9187..9381 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(9447..10520) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(10949..11578) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga 121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 241 cagtgccaag cttgtggatc ccccatcaca actttgtata gaaaagttgg ctccgaattc 301 gcccttagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa tacaacttta 361 gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact ccatttcctt 421 ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac tttatgtctt 481 ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac acaagttgct 541 gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac agcacagccc 601 atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca gctcttgcac 661 ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca cattttaatc 721 cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt ggagcttccc 781 atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt ctgagcctct 841 ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca 901 aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa 961 gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta 1021 tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact 1081 tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat 1141 tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga 1201 tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag 1261 acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa 1321 atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata 1381 aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg 1441 gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata 1501 cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat 1561 ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac 1621 ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt aataaacggc 1681 gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag cacaaatact 1741 tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta aacaacgctc 1801 aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc tcgtgaccta 1861 gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag ctcttcttct 1921 tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt ctctctaccg 1981 tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt gttttcgttc 2041 gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat cgaagacgat 2101 tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca tagatatcat 2161 ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt gtgatttcta 2221 tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa tctgagtttt 2281 tctgattaac agaagggcga attcgaccca agtttgtaca aaaaagcagg ctccgcggcc 2341 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcaaggata 2401 acaccgtgcc cctgaaactg attgctctgc tggctaatgg cgagttccat agtggcgaac 2461 agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt cagactctgc 2521 gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg cccgagccta 2581 tccagctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc 2641 tgcctgtgat cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg 2701 gggatgcttg cattgcagaa tatcagcagg caggaagagg agggagaggg aggaaatggt 2761 tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctggag cagggaccag 2821 cagcaatcgg cctgagcctg gtcatcggaa ttgtcatggc agaagtgctg cgaaagctgg 2881 gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg 2941 caggcatcct ggtggagctg accggaaaaa caggcgatgc tgcacagatc gtcattggcg 3001 ccgggattaa catggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca 3061 cactgcagga agcagggatt aacctggaca ggaatactct ggccgctatg ctgatccgag 3121 agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgtcacggt 3181 gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga 3241 ttttcgggat tagccgggga attgataaac agggagccct gctgctggaa caggacggaa 3301 ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag ctgcagctgc 3361 ctcccctgga gcgcctgacc ctggacggag gcggtggaag cggcggaggt tccatggtga 3421 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 3481 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 3541 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 3601 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg 3661 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 3721 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 3781 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 3841 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 3901 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 3961 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 4021 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 4081 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagggccca 4141 agggtgggcg cgccgaccca gctttcttgt acaaagtggt tgataacagc gcttagagct 4201 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 4261 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 4321 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 4381 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 4441 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca 4501 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4561 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt 4621 aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg cgcgcgataa 4681 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg 4741 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac 4801 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt 4861 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggggac 4921 gtccccagct cggtaccccc tcctgggcac ggtcgtacgc gatccgccgc ggttccacct 4981 caccattgcg ggtatgccag ttgagttcga tgagttcccg taccagctcc aactcgggcg 5041 atgccacgcg cactccgttc agatctcggt gacgggcagg accggacggg gcggtaccgg 5101 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc 5161 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc 5221 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag 5281 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac 5341 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat 5401 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac 5461 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt 5521 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg 5581 gatgtcggcc gggcgtcgtt ctgggctcat atcttattgc cccccgggat cagatctgga 5641 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga 5701 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc 5761 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg 5821 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc 5881 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca 5941 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc 6001 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag 6061 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct 6121 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc 6181 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga 6241 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca 6301 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat 6361 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac 6421 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc 6481 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga 6541 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc 6601 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa 6661 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt 6721 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg 6781 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc 6841 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa 6901 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat 6961 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag 7021 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg 7081 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc 7141 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct 7201 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag 7261 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt 7321 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt 7381 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag 7441 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg 7501 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc 7561 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca 7621 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa 7681 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag 7741 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt 7801 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc 7861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 7921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 7981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 8041 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 8101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 8161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 8221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 8281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 8341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 8401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 8461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 8521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 8581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 8641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 8701 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 8761 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 8821 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 8881 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 8941 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg 9001 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt 9061 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt 9121 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca 9181 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag 9241 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca 9301 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc 9361 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca 9421 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg 9481 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac 9541 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct 9601 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct 9661 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa 9721 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa 9781 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag 9841 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc 9901 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct 9961 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg 10021 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga 10081 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct 10141 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg 10201 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc 10261 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta 10321 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc 10381 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc 10441 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg 10501 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg 10561 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc 10621 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta 10681 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca 10741 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga 10801 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc 10861 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt 10921 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca 10981 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg 11041 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct 11101 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg 11161 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct 11221 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga 11281 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca 11341 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc 11401 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg 11461 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg 11521 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc 11581 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg 11641 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc 11701 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg 11761 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc 11821 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat 11881 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg 11941 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca 12001 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg 12061 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg 12121 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg 12181 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg 12241 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc 12301 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg 12361 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg 12421 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt 12481 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg 12541 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac 12601 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt 12661 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag 12721 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa 12781 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta 12841 aatatccgat tattctaata aacgctcttt tctcttag //