LOCUS Exported 9213 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Fluorescence reporter to monitor ER-phagy activity. ACCESSION . VERSION . KEYWORDS pCW57-CMV-ssRFP-GFP-KDEL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9213) AUTHORS Chino H, Hatta T, Natsume T, Mizushima N TITLE Intrinsically Disordered Protein TEX264 Mediates ER-phagy. JOURNAL Mol Cell. 2019 Apr 11. pii: S1097-2765(19)30257-6. doi: 10.1016/j.molcel.2019.03.033. PUBMED 31006538 REFERENCE 2 (bases 1 to 9213) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9213 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 17..134 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 202..472 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 505..543 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 540..564 /label=LNCX /note="Human CMV promoter, forward primer" sig_peptide 643..696 /product="Drosophila BiP signal sequence" /label=BiP signal sequence CDS 697..1371 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" primer_bind complement(827..845) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1095..1114 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1289..1312 /label=DsRed1-C /note="DsRed1, forward primer" CDS 1417..2133 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1417..2133 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1462..1483) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1723..1742) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2070..2091 /label=EGFP-C /note="EGFP, forward primer" promoter 2155..2665 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 2584..2602 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 2675..3271 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(2675..2694) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3171..3191 /label=Puro-F /note="Puromycin resistance gene, forward primer" CDS 3272..3325 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3326..4072 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" primer_bind complement(4112..4132) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4530..4541) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4746..4979 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5051..5172 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5088..5107) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5142..5161 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 5212..5347 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5274..5293 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5347..5418 /gene="bla" /label=AmpR promoter CDS 5419..6279 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5637..5656) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6450..7038 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6939..6958 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7192..7209 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7326..7347 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7362..7392 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7400..7416 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7405..7427 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7424..7440 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7424..7440 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7459..7479 /label=T3 /note="T3 promoter, forward primer" promoter 7461..7479 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7507..7733 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7734..7914 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7961..8086 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8579..8812 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8997..9041 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 aattctcgac ctcgagtttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa 61 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa 121 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcgaatcg 181 atatgtcctc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 241 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 301 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga 361 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca 421 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa 481 agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga 541 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat 601 agaagacacc gggaccgatc cagcctccgc ggccccgaat tcatgaagtt atgcatatta 661 ctggccgtcg tggcctttgt tggcctctcg ctcgggatgg cctcctccga ggacgtcatc 721 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag 781 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 841 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc 901 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 1021 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 1081 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 1141 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag 1201 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag 1261 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac 1321 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc catcgatgaa 1381 ttcactagtg ttaacggatc cctcgagcgg ccgcccatgg tgagcaaggg cgaggagctg 1441 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1501 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1561 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 1621 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1681 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1741 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1801 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 1861 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 1921 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1981 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 2041 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 2101 gggatcactc tcggcatgga cgagctgtac aagaaagatg agctctaagg atccggggtt 2161 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg 2221 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg 2281 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc 2341 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc 2401 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg 2461 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg 2521 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc 2581 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga 2641 ccgaatcacc gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg 2701 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 2761 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 2821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 2881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 2941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 3001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 3061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 3121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 3181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 3241 cacctggtgc atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg 3301 tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg 3361 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca 3421 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct 3481 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg 3541 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc 3601 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac 3661 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta 3721 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca 3781 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca 3841 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact 3901 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct 3961 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat 4021 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa 4081 ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg 4141 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4201 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4261 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 4321 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 4381 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 4441 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 4501 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 4561 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 4621 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc 4681 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 4741 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 4801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 4861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 4921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 4981 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 5041 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 5161 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 5221 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 5281 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 5341 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 5401 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 5461 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 5521 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 5581 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 5641 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 5701 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 5761 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 5821 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 5881 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 5941 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 6001 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 6061 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 6121 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 6181 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 6241 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 6301 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6361 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6421 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 6481 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 6541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 6601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 6661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 6721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 6841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 6901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 6961 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 7021 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 7081 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 7141 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 7201 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 7261 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 7321 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 7381 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 7441 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa 7501 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc 7561 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 7621 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg 7681 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc 7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 7921 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 7981 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 8041 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 8101 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 8161 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 8221 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 8281 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 8341 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 8401 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 8461 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 8521 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 8581 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 8641 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 8701 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 8761 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 8821 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 8881 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 8941 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 9001 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 9061 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 9121 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 9181 cgtttcagac ccacctccca accccgaggg gac //