LOCUS       Exported               10631 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  doxycycline-inducible expression of human GATA2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFUW-tetO-GATA2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Gomes AM, Kurochkin I, Chang B, Daniel M, Law K, Satija N, Lachmann 
            A, Wang Z, Ferreira L, Ma'ayan A, Chen BK, Papatsenko D, Lemischka 
            IR, Moore KA, Pereira CF
  TITLE     Cooperative Transcription Factor Induction Mediates Hemogenic 
            Reprogramming.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 
            10.1016/j.celrep.2018.11.032.
  PUBMED    30517869
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    82..100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        301..339
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     336..360
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2749..3337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2802..2822)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3220..3231)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3340..3356)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3341..3357)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3862..4042
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4068..4085)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4074..4298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4344..4772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4431..4450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4641..4662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4781..4801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4786..5115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4966..5101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5028..5047
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5163..5210
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5229..5603
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5733..5854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5770..5789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5824..5843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5903..5919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5903..5919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5916..5938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5927..5943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5951..5981)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5996..6017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6134..6151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6305..6893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6385..6404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7064..7924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7687..7706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7925..8029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8104..8123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8295..8674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8676..8874
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8829..8849
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8892..9072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9119..9244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9737..9970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10155..10199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10497..10614
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
       61 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      121 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga
      301 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
      361 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      421 ctccgcggcc ccgaattccg gggccggagc cgggagggcc cgaggccgag gcgcactcta
      481 cccccagctc ctaccctgta agccccgcca gcctccggac gtgctgtccc tgggcccgtc
      541 gccctcgggg ctcccgccgg aactccttca ctctcagagg ccgagtccct cccctcccca
      601 cggctgcgtg tggccgttgc cgtctgcacc cagaccctga gccgccgccg ccggccatgg
      661 aggtggcgcc cgagcagccg cgctggatgg cgcacccggc cgtgctgaat gcgcagcacc
      721 ccgactcaca ccacccgggc ctggcgcaca actacatgga acccgcgcag ctgctgcctc
      781 cagacgaggt ggacgtcttc ttcaatcacc tcgactcgca gggcaacccc tactatgcca
      841 accccgctca cgcgcgggcg cgcgtctcct acagccccgc gcacgcccgc ctgaccggag
      901 gccagatgtg ccgcccacac ttgttgcaca gcccgggttt gccctggctg gacgggggca
      961 aagcagccct ctctgccgct gcggcccacc accacaaccc ctggaccgtg agccccttct
     1021 ccaagacgcc actgcacccc tcagctgctg gaggccctgg aggcccactc tctgtgtacc
     1081 caggggctgg gggtgggagc gggggaggca gcgggagctc agtggcctcc ctcaccccta
     1141 cagcagccca ctctggctcc caccttttcg gcttcccacc cacgccaccc aaagaagtgt
     1201 ctcctgaccc tagcaccacg ggggctgcgt ctccagcctc atcttccgcg gggggtagtg
     1261 cagcccgagg agaggacaag gacggcgtca agtaccaggt gtcactgacg gagagcatga
     1321 agatggaaag tggcagtccc ctgcgcccag gcctagctac tatgggcacc cagcctgcta
     1381 cacaccaccc catccccacc tacccctcct atgtgccggc ggctgcccac gactacagca
     1441 gcggactctt ccaccccgga ggcttcctgg ggggaccggc ctccagcttc acccctaagc
     1501 agcgcagcaa ggctcgttcc tgttcagaag gccgggagtg tgtcaactgt ggggccacag
     1561 ccacccctct ctggcggcgg gacggcaccg gccactacct gtgcaatgcc tgtggcctct
     1621 accacaagat gaatgggcag aaccgaccac tcatcaagcc caagcgaaga ctgtcggccg
     1681 ccagaagagc cggcacctgt tgtgcaaatt gtcagacgac aaccaccacc ttatggcgcc
     1741 gaaacgccaa cggggaccct gtctgcaacg cctgtggcct ctactacaag ctgcacaatg
     1801 ttaacaggcc actgaccatg aagaaggaag ggatccagac tcggaaccgg aagatgtcca
     1861 acaagtccaa gaagagcaag aaaggggcgg agtgcttcga ggagctgtca aagtgcatgc
     1921 aggagaagtc atcccccttc agtgcagctg ccctggctgg acacatggca cctgtgggcc
     1981 acctcccgcc cttcagccac tccggacaca tcctgcccac tccgacgccc atccacccct
     2041 cctccagcct ctccttcggc cacccccacc cgtccagcat ggtgaccgcc atgggctagg
     2101 gaacagatgg acgtcgagga ccgggcactc ccgggatggg tggaccaaac ccttagcagc
     2161 ccagcatttc ccgaaggccg acaccactcc tgccagcccg gctcggccca gcaccccctc
     2221 tcctggaggg cgcccagcag cctgccagca gttactgtga atgttcccca ccgctgagag
     2281 gctgcctccg cacctgaccg ctgcccaggt ggggtttcct gcatggacag ttgtttggag
     2341 aacaacaagg acaactttat gtagagaaaa ggaggggacg ggacagacga aggcaaccat
     2401 ttttagaagg aaaaaggatt aggcaaaaat aatttatttt gctcttgttt ctaacaagga
     2461 cttggagact tggtggtctg agctgtccca agtcctccgg ttcttcctcg ggattggcgg
     2521 gtccacttgc cagggctctg ggggcagatt tgtggggacc tcagcctgca ccctcttctc
     2581 ctctggcttc cctctctgaa atagccgaac tccaggctgg gctgagccaa agccagagtg
     2641 gccacggccc agggagggtg agctggtgcc tgctttgacg ggccaggccc tggagggcag
     2701 agacaatcac gggcggtcct gcaggaattc gatatcaagc ttatcgataa tcaacctctg
     2761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3121 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3181 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3301 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     3361 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     3421 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     3481 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3541 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     3601 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     3661 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     3721 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     3781 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     3841 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3901 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3961 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4021 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4081 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4141 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4201 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4261 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4321 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4381 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4441 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4501 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4561 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4621 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4681 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4741 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4801 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4861 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4921 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5041 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5101 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5161 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5221 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5281 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     5341 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     5401 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     5461 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     5521 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     5581 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     5641 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5701 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5821 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5881 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5941 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6001 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6061 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6121 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6181 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8041 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8101 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8161 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8221 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8281 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     8341 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     8401 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     8461 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     8521 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     8581 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     8641 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     8701 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     8761 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     8821 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     8881 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8941 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9001 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9061 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9121 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9181 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9241 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9301 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9361 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9421 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9481 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9541 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     9601 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9661 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9721 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9781 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9841 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     9901 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     9961 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10021 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10081 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10141 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10201 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10261 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10321 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    10381 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    10441 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
    10501 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
    10561 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
    10621 tattacaggg a
//
