LOCUS Exported 13406 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae. ACCESSION . VERSION . KEYWORDS pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13406) TITLE Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast REFERENCE 2 (bases 1 to 13406) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13406 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 95..1030 /gene="Pichia pastoris AOX1" /label=AOX1 promoter /note="inducible promoter, regulated by methanol" promoter 1039..1515 /label=GAP promoter /note="promoter for Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase" CDS 2591..2620 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2636..2653 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 2733..2979 /gene="Pichia pastoris AOX1" /label=AOX1 terminator /note="transcription terminator for AOX1" promoter 2980..3456 /label=GAP promoter /note="promoter for Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase" CDS 5626..5655 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 5671..5688 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 5768..6014 /gene="Pichia pastoris AOX1" /label=AOX1 terminator /note="transcription terminator for AOX1" promoter 6015..6491 /label=GAP promoter /note="promoter for Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase" terminator 8088..8334 /gene="Pichia pastoris AOX1" /label=AOX1 terminator /note="transcription terminator for AOX1" CDS complement(9159..9974) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 9882..9901 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 9966..9985 /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind complement(10139..10158) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(11136..11158) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 11244..11384 /label=bom /note="basis of mobility region from pBR322" primer_bind 11299..11318 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(11399..11416) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11570..12158) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11650..11669) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12329..13189) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12952..12971 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13190..13294) /gene="bla" /label=AmpR promoter primer_bind 13362..13380 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atgtttgaca gcttatcatc gataagctga ctcatgttgg tattgtgaaa tagacgcaga 61 tcgggaacac tgaaaaataa cagttattat tcgagatcta acatccaaag acgaaaggtt 121 gaatgaaacc tttttgccat ccgacatcca caggtccatt ctcacacata agtgccaaac 181 gcaacaggag gggatacact agcagcagac cgttgcaaac gcaggacctc cactcctctt 241 ctcctcaaca cccacttttg ccatcgaaaa accagcccag ttattgggct tgattggagc 301 tcgctcattc caattccttc tattaggcta ctaacaccat gactttatta gcctgtctat 361 cctggccccc ctggcgaggt tcatgtttgt ttatttccga atgcaacaag ctccgcatta 421 cacccgaaca tcactccaga tgagggcttt ctgagtgtgg ggtcaaatag tttcatgttc 481 cccaaatggc ccaaaactga cagtttaaac gctgtcttgg aacctaatat gacaaaagcg 541 tgatctcatc caagatgaac taagtttggt tcgttgaaat gctaacggcc agttggtcaa 601 aaagaaactt ccaaaagtcg gcataccgtt tgtcttgttt ggtattgatt gacgaatgct 661 caaaaataat ctcattaatg cttagcgcag tctctctatc gcttctgaac cccggtgcac 721 ctgtgccgaa acgcaaatgg ggaaacaccc gctttttgga tgattatgca ttgtctccac 781 attgtatgct tccaagattc tggtgggaat actgctgata gcctaacgtt catgatcaaa 841 atttaactgt tctaacccct acttgacagc aatatataaa cagaaggaag ctgccctgtc 901 ttaaaccttt ttttttatca tcattattag cttactttca taattgcgac tggttccaat 961 tgacaagctt ttgattttaa cgacttttaa cgacaacttg agaagatcaa aaaacaacta 1021 attattcgaa gactagtctt tttgtagaaa tgtcttggtg tcctcgtcca atcaggtagc 1081 catctctgaa atatctggct ccgttgcaac tccgaacgac ctgctggcaa cgtaaaattc 1141 tccggggtaa aacttaaatg tggagtaatg gaaccagaaa cgtctcttcc cttctctctc 1201 cttccaccgc ccgttaccgt cgctaggaaa ttttactctg ctggagagct tcttctacgg 1261 cccccttgca gcaatgctct tcccagcatt acgttgcggg taaaacggag gtcgtgtacc 1321 cgacctagca gcccagggat ggaaaagtcc cggccgtcgc tggcaataat agcgggcgga 1381 cgcatgtcat gagattattg gaaaccacca gaatcgaata taaaaggcga acacctttcc 1441 caattttggt ttctcctgac ccaaagactt taaatttaat ttatttgtcc ctatttcaat 1501 caattgaaca actatatgtc tattccagaa actcaaaaag ccattatctt ctacgaatcc 1561 aacggcaagt tggagcataa ggatatccca gttccaaagc caaagcccaa cgaattgtta 1621 atcaacgtca agtactctgg tgtctgccac accgatttgc acgcttggca tggtgactgg 1681 ccattgccaa ctaagttacc attagttggt ggtcacgaag gtgccggtgt cgttgtcggc 1741 atgggtgaaa acgttaaggg ctggaagatc ggtgactacg ccggtatcaa atggttgaac 1801 ggttcttgta tggcctgtga atactgtgaa ttgggtaacg aatccaactg tcctcacgct 1861 gacttgtctg gttacaccca cgacggttct ttccaagaat acgctaccgc tgacgctgtt 1921 caagccgctc acattcctca aggtactgac ttggctgaag tcgcgccaat cttgtgtgct 1981 ggtatcaccg tatacaaggc tttgaagtct gccaacttga gagcaggcca ctgggcggcc 2041 atttctggtg ctgctggtgg tctaggttct ttggctgttc aatatgctaa ggcgatgggt 2101 tacagagtct taggtattga tggtggtcca ggaaaggaag aattgtttac ctcgctcggt 2161 ggtgaagtat tcatcgactt caccaaagag aaggacattg ttagcgcagt cgttaaggct 2221 accaacggcg gtgcccacgg tatcatcaat gtttccgttt ccgaagccgc tatcgaagct 2281 tctaccagat actgtagggc gaacggtact gttgtcttgg ttggtttgcc agccggtgca 2341 aagtgctcct ctgatgtctt caaccacgtt gtcaagtcta tctccattgt cggctcttac 2401 gtggggaaca gagctgatac cagagaagcc ttagatttct ttgccagagg tctagtcaag 2461 tctccaataa aggtagttgg cttatccagt ttaccagaaa tttacgaaaa gatggagaag 2521 ggccaaattg ctggtagata cgttgttgac acttctaaat aatcgagccg cggcggccgc 2581 cagctttcta gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca 2641 tcatcatcat cattgagttt gtagccttag acatgactgt tcctcagttc aagttgggca 2701 cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc 2761 tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt 2821 ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct 2881 gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta 2941 tttcccactc ctcttcagag tacagaagat taagtgagat ttttgtagaa atgtcttggt 3001 gtcctcgtcc aatcaggtag ccatctctga aatatctggc tccgttgcaa ctccgaacga 3061 cctgctggca acgtaaaatt ctccggggta aaacttaaat gtggagtaat ggaaccagaa 3121 acgtctcttc ccttctctct ccttccaccg cccgttaccg tccctaggaa attttactct 3181 gctggagagc ttcttctacg gcccccttgc agcaatgctc ttcccagcat tacgttgcgg 3241 gtaaaacgga ggtcgtgtac ccgacctagc agcccaggga tggaaaagtc ccggccgtcg 3301 ctggcaataa tagcgggcgg acgcatgtca tgagattatt ggaaaccacc agaatcgaat 3361 ataaaaggcg aacacctttc ccaattttgg tttctcctga cccaaagact ttaaatttaa 3421 tttatttgtc cctatttcaa tcaattgaac aactatatgt cgccctctgc cgtacaatca 3481 tcaaaactag aagaacagtc aagtgaaatt gacaagttga aagcaaaaat gtcccagtct 3541 gcctccactg cgcagcagaa gaaggaacat gagtatgaac atttgacctc ggtcaagatc 3601 gtgccacaac ggcccatctc agatagactg cagcccgcaa ttgctaccca ctattctcca 3661 cacttggacg ggttgcagga ctatcagcgc ttgcacaagg agtctattga agaccctgct 3721 aagttcttcg gttctaaagc tacccaattt ttaaactggt ctaagccatt cgataaggtg 3781 ttcatcccag actctaaaac gggtaggccc tccttccaga acaatgcatg gttcctcaac 3841 ggccaattaa acgcctgtta caactgtgtt gacagacatg ccttgaagac ccctaacaag 3901 aaagccatta ttttcgaagg tgacgagcct ggccaaggct attccattac ctacaaggaa 3961 ctacttgaag aagtttgtca agtggcacaa gtgctgactt actctatggg cgttcgcaag 4021 ggcgatactg ttgccgtgta catgcctatg gtcccagaag caatcataac cttgttggcc 4081 atttcccgta tcggcgccat tcactccgta gtctttgccg ggttttcttc caactccttg 4141 agagatcgta tcaacgatgg ggactctaaa gttgtcatca ctacagatga atccaacaga 4201 ggtggtaaag tcattgagac taaaagaatt gttgatgacg cgctaagaga gaccccaggc 4261 gtgagacacg tcttggttta tagaaagacc aacaatccat ctgttgcttt ccatgccccc 4321 agagatttag attgggcaac agaaaagaag aaatacaaga cctactatcc atgcacaccc 4381 gttgattctg aggatccatt attcttgttg tatacgtctg gttctactgg tgcccccaag 4441 ggtgttcaac attctaccgc aggttacttg ctgggagctt tgttgaccat gcgctacact 4501 tttgacactc accaagaaga cgttttcttc acagctggag acattggctg gattacaggc 4561 cacacttatg tggtttatgg tcccttacta tatggttgtg ccactttggt ctttgaaggg 4621 actcctgcgt acccaaatta ctcccgttat tgggatatta ttgatgaaca caaagtcacc 4681 caattttatg ttgccccaac tgctttgcgt ttgttgaaaa gagctggtga ttcctacatc 4741 gaaaatcatt ccttaaaatc tttgcgttgc ttgggttcgg tcggtgaacc aattgctgct 4801 gaagtttggg agtggtactc tgaaaaaata ggtaaaaatg aaatccccat tgtagacacc 4861 tactggcaaa cagaatctgg ttcgcatctg gtcaccccgc tggctggtgg tgtcacacca 4921 atgaaaccgg gttctgcctc attccccttc ttcggtattg atgcagttgt tcttgaccct 4981 aacactggtg aagaacttaa taccagccac gcagagggtg tccttgccgt caaagctgca 5041 tggccatcat ttgcaagaac tatttggaaa aatcatgata ggtatctaga cacttatttg 5101 aacccttacc ctggctacta tttcactggt gatggtgctg caaaggataa ggatggttat 5161 atctggattt tgggtcgtgt agacgatgtg gtgaacgtct ctggtcaccg tctgtctacc 5221 gctgaaattg aggctgctat tatcgaagat ccaattgtgg ccgagtgtgc tgttgtcgga 5281 ttcaacgatg acttgactgg tcaagcagtt gctgcatttg tggtgttgaa aaacaaatct 5341 aattggtcca ccgcaacaga tgatgaatta caagatatca agaagcattt ggtctttact 5401 gttagaaaag acatcgggcc atttgccgca ccaaaattga tcattttagt ggatgacttg 5461 cccaagacaa gatctggcaa aattatgaga cgtattttaa gaaaaatcct agcaggagaa 5521 agtgaccaac taggcgacgt ttctacattg tcaaaccctg gcattgttag acatccaatt 5581 gattcggtca agttgcctcg agccgcggcg gccgccagct ttctagaaca aaaactcatc 5641 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttgtagc 5701 cttagacatg actgttcctc agttcaagtt gggcacttac gagaagaccg gtcttgctag 5761 attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct tcatttttga 5821 tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg tttcttctcg 5881 tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg gtaggggttt 5941 gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt cagagtacag 6001 aagattaagt gagatttttg tagaaatgtc ttggtgtcct cgtccaatca ggtagccatc 6061 tctgaaatat ctggctccgt tgcaactccg aacgacctgc tggcaacgta aaattctccg 6121 gggtaaaact taaatgtgga gtaatggaac cagaaacgtc tcttcccttc tctctccttc 6181 caccgcccgt taccgtccct aggaaatttt actctgctgg agagcttctt ctacggcccc 6241 cttgcagcaa tgctcttccc agcattacgt tgcgggtaaa acggaggtcg tgtacccgac 6301 ctagcagccc agggatggaa aagtcccggc cgtcgctggc aataatagcg ggcggacgca 6361 tgtcatgaga ttattggaaa ccaccagaat cgaatataaa aggcgaacac ctttcccaat 6421 tttggtttct cctgacccaa agactttaaa tttaatttat ttgtccctat ttcaatcaat 6481 tgaacaacta tatgactaag ctacactttg acactgctga accagtcaag atcacacttc 6541 caaatggttt gacatacgag caaccaaccg gtctattcat taacaacaag tttatgaaag 6601 ctcaagacgg taagacctat cccgtcgaag atccttccac tgaaaacacc gtttgtgagg 6661 tctcttctgc caccactgaa gatgttgaat atgctatcga atgtgccgac cgtgctttcc 6721 acgacactga atgggctacc caagacccaa gagaaagagg ccgtctacta agtaagttgg 6781 ctgacgaatt ggaaagccaa attgacttgg tttcttccat tgaagctttg gacaatggta 6841 aaactttggc cttagcccgt ggggatgtta ccattgcaat caactgtcta agagatgctg 6901 ctgcctatgc cgacaaagtc aacggtagaa caatcaacac cggtgacggc tacatgaact 6961 tcaccacctt agagccaatc ggtgtctgtg gtcaaattat tccatggaac tttccaataa 7021 tgatgttggc ttggaagatc gccccagcat tggccatggg taacgtctgt atcttgaaac 7081 ccgctgctgt cacaccttta aatgccctat actttgcttc tttatgtaag aaggttggta 7141 ttccagctgg tgtcgtcaac atcgttccag gtcctggtag aactgttggt gctgctttga 7201 ccaacgaccc aagaatcaga aagctggctt ttaccggttc tacagaagtc ggtaagagtg 7261 ttgctgtcga ctcttctgaa tctaacttga agaaaatcac tttggaacta ggtggtaagt 7321 ccgcccattt ggtctttgac gatgctaaca ttaagaagac tttaccaaat ctagtaaacg 7381 gtattttcaa gaacgctggt caaatttgtt cctctggttc tagaatttac gttcaagaag 7441 gtatttacga cgaactattg gctgctttca aggcttactt ggaaaccgaa atcaaagttg 7501 gtaatccatt tgacaaggct aacttccaag gtgctatcac taaccgtcaa caattcgaca 7561 caattatgaa ctacatcgat atcggtaaga aagaaggcgc caagatctta actggtggcg 7621 aaaaagttgg tgacaagggt tacttcatca gaccaaccgt tttctacgat gttaatgaag 7681 acatgagaat tgttaaggaa gaaatttttg gaccagttgt cactgtcgca aagttcaaga 7741 ctttagaaga aggtgtcgaa atggctaaca gctctgaatt cggtctaggt tctggtatcg 7801 aaacagaatc tttgagcaca ggtttgaagg tggccaagat gttgaaggcc ggtaccgtct 7861 ggatcaacac atacaacgat tttgactcca gagttccatt cggtggtgtt aagcaatctg 7921 gttacggtag agaaatgggt gaagaagtct accatgcata cactgaagta aaagctgtca 7981 gaattaagtt gtaacctagg gcggccgcga attaattcgc cttagacatg actgttcctc 8041 agttcaagtt gggcacttac gagaagaccg gtcttgctag attctaatca agaggatgtc 8101 agaatgccat ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct 8161 atatagtata ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca 8221 gcctatctcg cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg 8281 atgtttttct tggtatttcc cactcctctt cagagtacag aagattaagt gagacgttcg 8341 tttgtgcaag cttatcgata agctttaatg cggtagttta tcacagttaa attgctaacg 8401 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac 8461 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat 8521 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat 8581 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt 8641 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt 8701 cctgtggatc tatcgaatct aaatgtaagt taaaatctct aaataaactt gaagtcggac 8761 agtgagtgta gtcttgagaa attctgaagc cgtattttta ttatcagtga gtcagtcatc 8821 aggagatcct ctacgccgga cgcatcgtgg ccgacctgca gggggggggg ggcgctgagg 8881 tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag 8941 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat 9001 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc 9061 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta 9121 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc 9181 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt 9241 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat 9301 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa 9361 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa 9421 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa 9481 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg 9541 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact 9601 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct 9661 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc 9721 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta 9781 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc 9841 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac 9901 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt 9961 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt 10021 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg 10081 ctttcccccc cccccctgca ggtcggcatc accggcgcca caggtgcggt tgctggcgcc 10141 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct 10201 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc 10261 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc 10321 ttcctaatgc aggagtcgca taagggagag cgtcgagtat ctatgattgg aagtatggga 10381 atggtgatac ccgcattctt cagtgtcttg aggtctccta tcagattatg cccaactaaa 10441 gcaaccggag gaggagattt catggtaaat ttctctgact tttggtcatc agtagactcg 10501 aactgtgaga ctatctcggt tatgacagca gaaatgtcct tcttggagac agtaaatgaa 10561 gtcccaccaa taaagaaatc cttgttatca ggaacaaact tcttgtttcg aactttttcg 10621 gtgccttgaa ctataaaatg tagagtggat atgtcgggta ggaatggagc gggcaaatgc 10681 ttaccttctg gaccttcaag aggtatgtag ggtttgtaga tactgatgcc aacttcagtg 10741 acaacgttgc tatttcgttc aaaccattcc gaatccagag aaatcaaagt tgtttgtcta 10801 ctattgatcc aagccagtgc ggtcttgaaa ctgacaatag tgtgctcgtg ttttgaggtc 10861 atctttgtat gaataaatct agtctttgat ctaaataatc ttgacgagcc agacgataat 10921 accaatctaa actctttaaa cgttaaagga caagtatgtc tgcctgtatt aaaccccaaa 10981 tcagctcgta gtctgatcct catcaacttg aggggcacta tcttgtttta gagaaatttg 11041 cggagatgcg atatcgagaa aaaggtacgc tgattttaaa cgtgaaattt atctcatgat 11101 ctctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 11161 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 11221 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 11281 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 11341 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 11401 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 11461 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 11521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 11581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 11641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 11701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 11761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 11821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 11881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 11941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 12001 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 12061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 12121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 12181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 12241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 12301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 12361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 12421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 12481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 12541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 12601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 12661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 12721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 12781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 12841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 12901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 12961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 13021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 13081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 13141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 13201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 13261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 13321 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 13381 cctttcgtct tcaagaatta attctc //