LOCUS Exported 9098 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Enocdes a histone H2B fused to the SunTag for single molecule imaging. ACCESSION . VERSION . KEYWORDS pcDNA4TO-H2B-SunTag24x_v1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9098) AUTHORS Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD TITLE A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. JOURNAL Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PUBMED 25307933 REFERENCE 2 (bases 1 to 9098) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9098 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 586..604 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 607..625 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 635..659 /label=LNCX /note="Human CMV promoter, forward primer" CDS 788..1165 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" CDS 1235..1300 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1316..1381 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1397..1462 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1478..1543 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1559..1624 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1640..1705 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1721..1786 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1802..1867 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1883..1948 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 1964..2029 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2045..2110 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2126..2191 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" primer_bind 2207..2229 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" primer_bind 2230..2252 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS 2255..2320 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2336..2401 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2417..2482 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2498..2563 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2579..2644 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2660..2725 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2741..2806 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2822..2887 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2903..2968 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 2984..3049 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 3065..3130 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" CDS 3146..3211 /codon_start=1 /product="GCN4 peptide that allows binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v1 /translation="LLPKNYHLENEVARLKKLVGER" misc_feature 3282..3834 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3426..3443) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3653..3672 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3846..4445 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(3846..3865) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4342..4362 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(4483..4500) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4489..4713 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4759..5187 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4846..4865) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5056..5077 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5196..5216) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5201..5530 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5381..5516 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5443..5462 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5578..5625 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 5644..6018 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6148..6269 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6185..6204) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6239..6258 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6318..6334) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6318..6334) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6331..6353) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6342..6358 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6366..6396) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6411..6432 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7109..7697) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7868..8728) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8491..8510 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8729..8833) /gene="bla" /label=AmpR promoter primer_bind complement(8908..8927) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata 601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg 661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccggc gcgccaccgg 781 tgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc 841 ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta 901 ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa 961 ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc 1021 ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc 1081 cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc 1141 catcaccaag tacaccagcg ctaaggatcg atccggtgga ggtggaggtc ctgcaggttc 1201 gaaggtacaa agcggtccgg gaggttctgg aggattgttg cccaaaaact accacttgga 1261 gaacgaggtc gcaaggttga aaaaattggt cggtgagcgt ggcggctcag gcggccttct 1321 tccaaaaaat tatcatcttg aaaatgaagt tgctagactt aaaaaacttg ttggagaaag 1381 aggcgggagc ggcggcctgc tgcccaagaa ctaccacctg gagaacgagg tcgcccgcct 1441 gaagaagctg gtcggcgagc gcggtggttc tggaggtttg ctcccaaaga actaccactt 1501 ggaaaacgaa gtcgcaagat tgaagaagtt ggtcggtgaa agaggaggaa gcggaggatt 1561 attacctaaa aattatcatt tggaaaatga agttgctaga ttgaagaagt tggttggaga 1621 gagagggggg tctggaggac tactaccgaa aaactaccac ctagagaacg aggtggcccg 1681 actaaaaaag ctagtgggcg agcgcggcgg cagtggcggc ctcctcccca agaattacca 1741 cctcgaaaat gaagtagcaa ggctcaagaa gctcgtagga gaaagaggtg gttccggggg 1801 actccttcca aaaaactatc atcttgaaaa cgaagttgca cgtcttaaaa aacttgttgg 1861 cgaacgtggc ggctctggcg ggctgctgcc caagaattac cacctggaaa atgaagtggc 1921 ccgcctgaag aagctggtgg gcgaacgcgg aggcagtgga ggcctactac cgaagaacta 1981 ccacctagag aacgaggtgg cacgcctaaa gaagctagtg ggcgagcgcg gcggctcggg 2041 tggtttatta cctaagaatt atcatttaga aaatgaagta gctcgattaa agaagttagt 2101 aggtgaacgt ggtgggtctg gtggtcttct tccgaagaac taccaccttg agaacgaggt 2161 agctcgtctt aagaaacttg taggtgagcg tggtggaagc ggtggtgggc tggcaagcca 2221 cgtttggtgc cgggagctgc atgtgtcaga ggaacttttg ccaaagaatt atcatcttga 2281 gaacgaagtg gctcgtctta agaaactcgt aggagaaaga ggtggcagtg gaggtctgct 2341 tcccaagaat taccacctgg aaaatgaggt agctagactg aaaaagctcg taggcgagcg 2401 cgggggaagt gggggattgc tgccaaaaaa ttatcatttg gagaacgaag tagcacgact 2461 aaagaaatta gttggggaga gggggggatc gggtggctta ctcccgaaaa attatcatct 2521 cgaaaacgaa gtggctcggc taaaaaagtt agttggagag cgtggcggtt ctggaggcct 2581 attaccaaaa aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagc tggtcggaga 2641 acggggaggc agtggtggcc tactacccaa gaattatcat cttgagaacg aggtagcgcg 2701 tttgaagaag ctcgtcggtg aaagaggtgg ctcaggaggg ctgctcccaa agaactatca 2761 tcttgaaaat gaggtcgctc gattaaaaaa gttagtgggc gagaggggag gcagtggtgg 2821 gctacttcct aagaattacc acctcgaaaa cgaagtagct cgattaaaga aattggtcgg 2881 tgagagaggt gggtcgggtg gcttactgcc gaaaaattat catctggaaa atgaggtagc 2941 gagactaaaa aagctcgtag gtgagagagg gggttctggc ggcttgctac caaaaaatta 3001 tcatcttgag aacgaagttg ctaggctcaa aaagctggtt ggagagcgcg gcggctcagg 3061 cggtctgtta cccaaaaact accacttgga aaatgaggtt gccagattga agaaactggt 3121 cggcgagcgc ggtggaagtg gaggactact tcctaaaaat tatcatttgg agaacgaagt 3181 cgctagactg aaaaagttgg ttggggaacg aggagggtct ggcggcaagc tagtcgaatt 3241 cgacacgtag gctagcttag taggcggccg ccccccccct aacgttactg gccgaagccg 3301 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 3361 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 3421 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 3481 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 3541 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 3601 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 3661 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 3721 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 3781 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca 3841 caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 3901 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 3961 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 4021 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 4081 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 4141 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 4201 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 4261 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 4321 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 4381 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 4441 cctgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 4501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 4561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 4621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 4681 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 4741 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 4801 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 4861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 4921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 4981 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 5041 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 5101 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 5161 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 5221 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 5281 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 5341 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 5401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 5461 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 5521 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt 5581 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa 5641 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg 5701 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc 5761 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg 5821 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg 5881 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag 5941 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg 6001 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa 6061 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 6121 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 6181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 6241 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 6301 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 6361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 6421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 6481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 6541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6901 gtggcgcttt ctcatagctc acgctgtagg tatcttcctc gctcactgac tcgctgcgct 6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 8881 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 8941 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 9001 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt 9061 tgcgctgctt cgcgatgtac gggccagata tacgcgtt //