LOCUS Exported 14102 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Genome editing for Lactobacillus plantarum WCSF1. ACCESSION . VERSION . KEYWORDS pHSP02 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14102) AUTHORS Huang H, Song X, Yang S TITLE Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. JOURNAL Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20. PUBMED 30927506 REFERENCE 2 (bases 1 to 14102) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14102 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(319..1134) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1042..1061 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1126..1145 /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1431..5537 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" misc_RNA complement(12299..12374) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(12549..13499) /codon_start=1 /gene="rep101 encoding an A56V mutation" /product="temperature-sensitive version of the RepA protein needed for replication with the pSC101 origin (Armstrong et al., 1984)" /label=Rep101(Ts) /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" rep_origin 13547..13769 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" ORIGIN 1 ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt 61 ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag 121 taaggcagcg gtatcatcaa cggggtctga cgctcagtgg aacgaaaact cacgttaagg 181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt acgtttccaa 301 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 361 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 421 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 481 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 541 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca 601 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 661 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca 721 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 781 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt 841 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 901 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 961 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt 1021 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 1081 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc 1141 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc 1201 ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcctgca gcgaaaagcc 1261 ctgacaaccc ttgttcctaa aaaggaataa gcgttcggtc agtaaataat agaaataaaa 1321 aatcagacct aagactgatg acaaaaagag aaaattttga taaaatagtc ttagaattaa 1381 attaaaaagg gaggccaaat ataatgaaaa atatgaatga caatgatgtt atggataaga 1441 aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg atcactgatg 1501 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca 1561 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc 1621 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg 1681 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt 1741 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag 1801 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag 1861 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt 1921 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac 1981 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa 2041 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa 2101 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt 2161 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat 2221 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag 2281 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag 2341 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac 2401 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc 2461 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg 2521 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg 2581 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga 2641 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga 2701 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct 2761 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat 2821 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata 2881 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa 2941 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat 3001 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac 3061 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat 3121 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg 3181 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata 3241 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga 3301 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg 3361 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc 3421 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga 3481 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat 3541 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata 3601 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg 3661 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg 3721 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa 3781 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata 3841 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg 3901 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac attgttccac 3961 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc 4021 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga 4081 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg 4141 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa 4201 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg 4261 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt 4321 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg 4381 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac 4441 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta 4501 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga 4561 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg 4621 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc 4681 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg 4741 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa 4801 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc 4861 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac 4921 tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag 4981 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt 5041 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa 5101 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa 5161 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc 5221 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag 5281 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg 5341 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt 5401 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag 5461 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc 5521 agctaggagg tgactgaagt atattttaga tgaagattat ttcttaatct aggctcgaga 5581 tctatcgata agcttaagtg attagtcaaa gaatggtgat gacaattgta aattctattt 5641 aatcactttg actagcaaat actaacaaca agacacacac accaaaaatc aaaaattcac 5701 tacttttagt taaaaaccac gtaaccacaa gaactaatcc aatccatgta atcgggttct 5761 tcaaatattt ctccaagatt ttcctcctct aatatgctca acttaaatga cctattcaat 5821 aaatctatta tgctgctaaa tagtttatag gacaaataag tatactctaa tgacctataa 5881 aagatagaaa attaaaaaat caagtgttcg cttcgctctc actgcccctc gacgttttag 5941 tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa gttttcgggc tactctctcc 6001 ttctccccct aataattaat taaaatctta ctctgtatat ttctgctaat cattcgctaa 6061 acagcaaaga aaaaacaaac acgtatcata gatataaatg taatggcata gtgcgggttt 6121 tattttcagc ctgtatcata gctaaacaaa tcgagttgtg tgtccgtttt agggcgttct 6181 gctagcttgt ttaaagtctc ttgaatgaat gtatgctcta agtcaaaaga atttgtcagc 6241 gcctttatat agctttcttt ttcttctttt tttactttaa tgatcgatag caacaatgat 6301 ttaacactag caagttgaat gccaccattt cttcctggtt taatcttaaa gaaaatttcc 6361 tgattcgcct tcagtacctt cagcaattta tctaatgtcc gttcaggaat gcctagcact 6421 tctctaatct cttttttggt cgtcactaaa taaggcttgt atacatcgct tttttcgcta 6481 atataagcca ttaaatcttc tttccattct gacaaatgaa cacgttgacg ttcgcttctt 6541 tttttcttga atttaaacca cccttgacgg acaaataaat ctttactggt taaatcactt 6601 gatacccaag ctttgcaaag aatggtaatg tattccctat tagccccttg atagttttct 6661 gaataggcac ttctaacaat tttgattact tctttttctt ctaagggttg atctaatcga 6721 ttattaaact caaacatatt atattcgcac gtttcgattg aatagcctga actaaagtag 6781 gctaaagaga gggtaaacat gacgttatta cgccctatta aacccttttc tcctgaaaat 6841 ttcgtttcgt gcaataagag attaaaccag ggttcatcta cttgtttttt gccttctgta 6901 ccgcttaaaa ccgttagact tgaacgagta aagcccttat tatctgtttg tttgaaagac 6961 caatcttgcc attctttgaa agaataacgg taattaggat caaaaaattc tacattgtcc 7021 gttcttggta tgcgagcaat accaaaatga ttacacgtta gatcaactgg caaagacttt 7081 ccaaaatatt ctcggatatt ttgcgaaatt attttggctg ctttgacaga tttaaattct 7141 gattttgaag tcacatagac tggcgtttct aaaacaaaat atgcttgata acctttatca 7201 gatttgataa tcatagtagg cataaaacct aaatcaatag cggttgttaa aatatcgctt 7261 gctgaaatag tttcttttgc cgtgtgaata tcaaaatcaa taaagaaggt attgatttgt 7321 cttaaattgt tttcagaatg tcctttcgtg tatgaacggt tttcgtctgc atacgttcca 7381 taacgataaa cgttgggtgt ccaatgtgta aatgtatctt gattttcttg aatcgcttcc 7441 tcggaagtca gaacaacacc acgaccgcca atcatgcttg attttgagcg atacgcaaaa 7501 atagcccctt tgcttttacc tggcttggta gtgattgagc gaattttact atttttaaat 7561 ttgtacttta acaagccgtc atgaagcaca gtttctacaa caaaagggat attcattcag 7621 ctgttctcct ttcctataaa tcctataaaa taggttgttt aattaacttg gtttgctttt 7681 tcattcaact gtttcaatat tgcatgtttt gaaaaagatt tttttccttt ataagtcaat 7741 ttttttccac taatcgaata aattattttg ttattttcta ttaacttata tatataatct 7801 tccccctccg aagaaaaata cttatctgat tttgtttcta agtagatatt tctcttttct 7861 aactctttct taaacgtttc tagtgtatag atatttgcta attttcttat ctccaataaa 7921 ctatttttta tataagtttt acattcatca tgattcatac aaactccacc ttctataaat 7981 gaatacaaaa aaagcaatca aacgatttcc gattgattgc ttaacaattc ttaaattcag 8041 tagcttagat acttgaaaac tctctgattt ccctatataa tgatagtacg gttatatacc 8101 gtcttcaaac aaagttaatt aaataacttc ttacgaggga agagttcatc tgactaactg 8161 ataagcgttg gtttggcaat cttatcgggc tatgcattta taaaatgtcg tcaaacattt 8221 tataaatgtg tcatggctct tttttcgttt ctattcagtt cgttgtttcg ttatatctag 8281 tataccgctt ttaaaaaaaa taagcaacga tttcgtgcat tattcacacg aagtcattgc 8341 ttttttcttc ttccatttct aaatccaatg ttacttgttc tgattctgtt tctggttctg 8401 gttctgttgg ctcatttggg attaaatcca ctactagcgt tgagttagtt aactttgcaa 8461 tttgttctag tgtttttatg gttggatctg attttcctga ttctattcgt gaataatttg 8521 atctactcat ttctaattct tggggtaccg ccagcatttc ggaaaaaaac cacgctaagg 8581 attttttcta taaaaagagc cgttatatta agaataaaac ggctctttta tacgtaaagg 8641 acgtaaattc atttgcccag tgtcatgtaa tccttcaaat ttgtattctc caagaaaatt 8701 gatatgttcc catcctaacg gccacgcata tggcattaaa tcttctctaa attctcctct 8761 tgcttttaat tcttctacgg ctttttccat atatacagtg ttccacacac ttatagcgtt 8821 aataattatg tttagtgcac tagctctttg taactggtct tggagagcac gttctctaaa 8881 ttctccacgt tgtccaaaaa atataattct agctaatgca ttgattgctt ctcctttatt 8941 taaacctttt tgaacccgtc tccttacggc tttattagat atgtaatcca gcgtaaagag 9001 ggttttctcg attcgtccca tttctccaag tgctgttgcg agtttatttt gtcttgcata 9061 tgatccgagc ttccccatga taagagcgct agggacctct ttagctcctt ggaagctgtc 9121 agtagtatac ctaataattt atctacattc cctttagtaa cgtgtaactt tccaaattta 9181 caaaagcgac tcatagaatt atttcctccc gttaaataat agataactat taaaaataga 9241 caatacttgc tcataagtaa cggtacttaa attgtttact ttggcgtgtt tcattgcttg 9301 atgaaactga tttttagtaa acagttgacg atattctcga ttgacccatt ttgaaacaaa 9361 gtacgtatat agcttccaat atttatctgg aacatctgtg gtatggcggg taagttttat 9421 taagacactg tttacttttg gtttaggatg aaagcattcc gctggcagct taagcaattg 9481 ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt cggtgaatat ccaaggtacg 9541 cttgtagaat ccttcttcaa caatcagata gatgtcagac gcatggcttt caaaaaccac 9601 ttttttaata atttgtgtgc ttaaatggta aggaatactc ccaacaattt tatacctctg 9661 tttgttaggg aattgaaact gtagaatatc ttggtgaatt aaagtgacac gagtattcag 9721 ttttaatttt tctgacgata agttgaatag atgactgtct aattcaatag acgttacctg 9781 tttacttatt ttagccagtt tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac 9841 ggtatcggtt tcttttaaat tcaattgttt tattatttgg ttgagtactt tttcactcgt 9901 taaaaagttt tgagaatatt ttatattttt gttcatgtaa tcactccttc ttaattacaa 9961 atttttagca tctaatttaa cttcaattcc tattatacaa aattttaaga tactgcacta 10021 tcaacacact cttaagtttg cttctaagtc ttatttccat aacttctttt acgtttccgc 10081 cattctttgc tgtttcgatt tttatgatat ggtgcaagtc agcacgaaca cgaaccgtct 10141 tatctcccat tatatctttt tttgcactga ttggtgtatc atttcgtttt tctttttgtg 10201 cgcttcttga taaaagggat agtaattcat tcctggttgc aaattttgaa aaccgctacg 10261 gatcacatct ttttctaaac tagggcccat tagttgccga ctacgcgggc tgatacctgc 10321 taaaattgtt tgaaagtcgg gcgtttgctc aacaatcgtt tcaatatcca ttctgtcgtt 10381 tcctcccggg ctagttatat tgattcataa ctgccgtaaa atcatcattt gctaaccaag 10441 cttcgaccgc cgctagcgcc gtaatcttag catcgtcaaa cttcgccact tcggctggcg 10501 taaatttccc caacacgtaa tcaacaacac tcatcttctg aggatggtca ataccaactt 10561 taacgcgctt gaagtgctga tcaccaacat gactaataat gctcttgatg ccattatgac 10621 cacccgccga tcccttttgt ttgaggcgta cttttcctag tggtaaatct aaatcatcat 10681 gtataacgag caaatcagca ggatcaacat tgtaatagct catcaacggt cccaccgctc 10741 gtccagaatc attcatataa gtggcgggtt tcaccagtaa aatcttctca ccgtcttgaa 10801 aagccgttgc cacctgtgct tcaaatttac tcgtttgaaa cgtaacgttt agtttgctag 10861 ctaattcgtc cacaatcata aaaccaacat tatgacgtgt ctgggcatat tgccgcccaa 10921 tgttgcctaa cccaacgatc attttcataa ttttatcttc tcctattact ttgcatacca 10981 aaacaggccg aaccggtaat cgacccgatt cggcaactct gagtaacgat accacctaag 11041 tcgtattggc accactactc acaccgtgac cgacgcgccc gccagtcaag tgttcaaaag 11101 ttagcgttta ttaagtgcga taagtatacc acaaagggct tattgacgcc cgccaaaggg 11161 ttttgcggac attgttaata attgtattaa aagcatgctc aatctaacac ttattttgca 11221 caaacatggt atactttaac cgtaaaaact aaattttcac tacgagagga tgacttattt 11281 tgtcaagcaa taaataatca tttcatacga ttaaatgtat gatgaacgct cgtctatagc 11341 agacgggcgt ttttttgttt gcttgaggta ccttagcgat tcattaaagc gcaacacgca 11401 ctaaaggcta tttttaaaac tttcttatca cgattaccgg ccttgaagtt tgcactcatc 11461 tcacttctgt tataaggtga gaatattacg aatatatgga ggaccaactt aattatgaaa 11521 cataaacgtg gacttatgat tgggggcagt atcctcggtc tcctcgtcat cggatatgtt 11581 gccacgagct taatctggca acatcaacaa acgttcctca gtaatacgtc ggtcgctggt 11641 gttgacgtca gtggtaaaac cgccgctcaa gcagcaccga aagttaatcg cgctttagaa 11701 catcgcactt atcgaattat tgaaaacggc caaacaaagt actcatttac cagcaaatca 11761 gccggtatta caatcaatac taaaaaagat ttaaccaaat tagtgaccaa acaaaactac 11821 tggcgttggc cccttgcact attgcaatcg gctcaggctg atgatagctc agcggttgga 11881 caactaacca tcaatcacac tgatttgaaa accctactac aaaaaatcac cacgactgcc 11941 aatcaaacca aacgaacaaa aactgaaaac gccaagttag tttatcaaca taaccaagtt 12001 gagattaaga aagaagttca aggaaccgaa ttagatcaag ctaaacttaa gacggtcgtc 12061 accaaagcaa ttagtaatgg tgacagtcat atcaccctta aagacgccta cgtcactccg 12121 accgttacca gcacgagtgc tagtttaaag acggccaaag caaaatcagc gaaatacgct 12181 accgaaaagg ccacctataa tatcaacggt cataaattca cgattcctca cgacctaatt 12241 ctcagttgga ttaaagtcga taagcaaggc aaagtaagtt tagaccaact caaaaaaagc 12301 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt 12361 tctagctcta aaactagcga catcgcgaac tggatctaga atatatcata gtatgtccat 12421 tctgtcaaca actatagcgc tgtcgacgta tggacagttt tccctttgat atgtaacggt 12481 gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca 12541 taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt 12601 tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat 12661 tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt 12721 agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt 12781 tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg 12841 aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa 12901 gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg 12961 tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc 13021 ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat 13081 ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa 13141 agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca 13201 tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat ttccacagtt 13261 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg 13321 ttcatcatct gaacgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg 13381 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc 13441 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata 13501 catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga 13561 taattactag tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct 13621 ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt 13681 tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa aaagataaaa 13741 agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca gtattacaaa 13801 aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta 13861 agtagcaccc tcgcaagctc ggttgcggcc gcaatcgggc aaatcgctga atattccttt 13921 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc 13981 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg 14041 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg 14101 cg //