LOCUS Exported 7204 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.. ACCESSION . VERSION . KEYWORDS pmax pona 12xTRICK 24xMS2SL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7204) AUTHORS Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA TITLE Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. JOURNAL Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. PUBMED 25792328 REFERENCE 2 (bases 1 to 7204) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7204 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..239 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind 186..207 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" intron 289..421 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" CDS 529..555 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 562..588 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 595..621 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(3586..3605) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" polyA_signal 3615..3696 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind 3640..3659 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin complement(3894..4482) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3974..3993) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4563..5357) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4674..4693) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 5284..5303 /label=Neo-R /note="Neomycin resistance gene, reverse primer" ORIGIN 1 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag 61 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca 121 atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat 181 caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag acaactctga 241 atactttcaa aagttaccga gaaagaagaa ctcagacacg gatcacaggt aagtatcaag 301 gttacaagac aggtttaagg aggccaatag aaactgggct tgtcgagaca gagaagattc 361 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 421 ggggtaccga agccgctagc gctaccggtc gccaccatgt cgcggaggcg gcacagctac 481 gagaacgatg gtggacaacc tcacaaaagg aggaagacgt ctgttgagta cccttatgat 541 gtccctgact acgctgccga gtatccctac gacgtccccg attatgccgc agaataccca 601 tacgatgtgc cagactacgc agctgagggg gggcaagtcc agttgcaaga atctggacct 661 ggtttggtta aaccatctga gacactttcc ctaacctgca cagtcagtgg cgcatcaatt 721 tcctcctcgc actattattg gggttggatt agacaaccac ctggaaaggg cccagagtgg 781 atagggtcta tgtactactc gggtagaact tactataatc ctgccttgaa gtcacgtgtt 841 accatatcct ctgataagtc aaaaaaccaa ttctttttaa aactcactag cgttaccgct 901 gctgatacag ctgtctatta tgctgcccgt gagggcccaa cgcattatta tgataattca 961 ggcccaatac ctagcgatga gtattttcaa cattggggcc agggcacttt agttacagtt 1021 tccggtggtg gcggctctgg tggcggtggc agcggcggtg gtggttccgg aggcggcggt 1081 tctcaggtgc agctgcagga gtcgggccca ggactggtga agccttcgga gaccctgtcc 1141 ctcacctgca ctgtctctgg tgcctccatc agcagtagtc attactactg gggctggatt 1201 cgccagcccc cagggaaggg gcctgagtgg attgggagta tgtattatag tgggagaacg 1261 tactacaacc cggccctcaa gagtcgagtc accatatcat cagacaagtc gaagaaccag 1321 ttcttcttga agttgacctc tgtaaccgcc gcggacacgg ccgtgtatta cgctgcgagg 1381 gagggaccca cacattacta tgataatagt ggtccaatac cttcggatga gtatttccag 1441 cactggggcc agggtaccct ggtcactgtc tccgtcgaca gatcctacct gcaaagcacc 1501 agaggatatg gcctcggtgc ctcagcatct tcagatgtgc tgtcactgtc caatcttcaa 1561 gccagcagag catatgggct cgctggctcc actgtcatta cagtggacct gatcttctcc 1621 aatcaaagga gcagaggata tggcctcgct ccctccgact acatcaggct ggaagtgaca 1681 tccatcattc atcccagcag agcatatggg ctcgctggga ccacagcatg cctcgaagtg 1741 ctgagagact atcatcaaac cgacagagga tatggcctcg tcggctccta ctccctcctg 1801 gtggaatact tctccactct tcaacccagc agagcatatg ggctcgctgg gtccatcaga 1861 gatacaagat ctctcgacag atcctacctg caaagcacca gaggatatgg cctcggtgcc 1921 tcagcatctt cagatgtgct gtcactgtcc aatcttcaag ccagcagagc atatgggctc 1981 gctggctcca ctgtcattac agtggacctg atcttctcca atcaaaggag cagaggatat 2041 ggcctcgctc cctccgacta catcaggctg gaagtgacat ccatcattca tcccagcaga 2101 gcatatgggc tcgctgggac cacagcatgc ctcgaagtgc tgagagacta tcatcaaacc 2161 gacagaggat atggcctcgt cggctcctac tccctcctgg tggaatactt ctccactctt 2221 caacccagca gagcatatgg gctcgctggg tccatcagag atacaagatc tctcgagtga 2281 taaggatcct acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg 2341 aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac 2401 ggtacttatt gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg 2461 acgacgatca cgcgtcgctc cagtattcca gggttcatca gatcctacgg tacttattgc 2521 caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg 2581 cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca agaaagcacg 2641 agcatcagcc gtgcctccag gtcgaatctt caaacgacga cgatcacgcg tcgctccagt 2701 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag catcagccgt 2761 gcctccaggt cgaatcttca aacgacgacg atcacgcgtc gctccagtat tccagggttc 2821 atcagatcct acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg 2881 aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac 2941 ggtacttatt gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg 3001 acgacgatca cgcgtcgctc cagtattcca gggttcatca gatcctacgg tacttattgc 3061 caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg 3121 cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca agaaagcacg 3181 agcatcagcc gtgcctccag gtcgaatctt caaacgacga cgatcacgcg tcgctccagt 3241 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag catcagccgt 3301 gcctccaggt cgaatcttca aacgacgacg atcacgcgtc gctccagtat tccagggttc 3361 atcagatcct acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg 3421 aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac 3481 ggtacttatt gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg 3541 acgacgatca cgcgtcgctc cagtattcca gggttcatca gatccgatga gtttggacaa 3601 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 3661 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 3721 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 3781 tgtggtactt aagaggggga gaccaaaggg cgagacgtta aggcctcacg tgacatgtga 3841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 4021 gttccgaccc tgccgcttac gggatacctg tccgcctttc tcccttcggg aagcgtggcg 4081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 4141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 4201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 4261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 4321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 4381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 4441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgccg 4561 tctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg 4621 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca 4681 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg 4741 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc 4801 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc 4861 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga 4921 gtacgtgctc tctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca 4981 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg 5041 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct 5101 tcagtgacaa cgtcgagtac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc 5161 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga 5221 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt 5281 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat 5341 ccatcttgtt caatcataat attattgaag catttatcag ggttcgtctc gtcccggtct 5401 cctcccatgc atggacagca gagatccagt ttggactagt agatctcggc cgcatattaa 5461 gtgcattgtt ctcgataccg ctaagtgcat tgttctcgtt agctcgatgg acaagtgcat 5521 tgttctcttg ctgaaagctc gatggacaag tgcattgttc tcttgctgaa agctcgatgg 5581 acaagtgcat tgttctcttg ctgaaagctc agtacccggg tcggagtacg gatctcggcc 5641 gcatattaag tgcattgttc tcgataccgc taagtgcatt gttctcgtta gctcgatgga 5701 caagtgcatt gttctcttgc tgaaagctcg atggacaagt gcattgttct cttgctgaaa 5761 gctcgatgga caagtgcatt gttctcttgc tgaaagctca gtacccgggt cggagtacgg 5821 atctcggccg catattaagt gcattgttct cgataccgct aagtgcattg ttctcgttag 5881 ctcgatggac aagtgcattg ttctcttgct gaaagctcga tggacaagtg cattgttctc 5941 ttgctgaaag ctcgatggac aagtgcattg ttctcttgct gaaagctcag tacccgggtc 6001 ggagtacgga tctcggccgc atattaagtg cattgttctc gataccgcta agtgcattgt 6061 tctcgttagc tcgatggaca agtgcattgt tctcttgctg aaagctcgat ggacaagtgc 6121 attgttctct tgctgaaagc tcgatggaca agtgcattgt tctcttgctg aaagctcagt 6181 acccgggtcg gagtacggat ctcggccgca tattaagtgc attgttctcg ataccgctaa 6241 gtgcattgtt ctcgttagct cgatggacaa gtgcattgtt ctcttgctga aagctcgatg 6301 gacaagtgca ttgttctctt gctgaaagct cgatggacaa gtgcattgtt ctcttgctga 6361 aagctcagta cccgggtcgg agtacggatc tcggccgcat attaagtgca ttgttctcga 6421 taccgctaag tgcattgttc tcgttagctc gatggacaag tgcattgttc tcttgctgaa 6481 agctcgatgg acaagtgcat tgttctcttg ctgaaagctc gatggacaag tgcattgttc 6541 tcttgctgaa agctcagtac ccgggtcgga gtacggatct cggccgcata ttaagtgcat 6601 tgttctcgat accgctaagt gcattgttct cgttagctcg atggacaagt gcattgttct 6661 cttgctgaaa gctcgatgga caagtgcatt gttctcttgc tgaaagctcg atggacaagt 6721 gcattgttct cttgctgaaa gctcagtacc cgggtcggag tacggatctc ggccgcatat 6781 taagtgcatt gttctcgata ccgctaagtg cattgttctc gttagctcga tggacaagtg 6841 cattgttctc ttgctgaaag ctcgatggac aagtgcattg ttctcttgct gaaagctcga 6901 tggacaagtg cattgttctc ttgctgaaag ctcagtaccc gggtcggagt acggatctcg 6961 gccgcatatt aagtgcattg ttctcgatac cgctaagtgc attgttctcg ttagctcgat 7021 ggacaagtgc attgttctct tgctgaaagc tcgatggaca agtgcattgt tctcttgctg 7081 aaagctcgat ggacaagtgc attgttctct tgctgaaagc tcagtacccg ggtcggagta 7141 ctgccccgcc cctagcgatt agccccggcc ccgcatagct ccgccccggg agtaccctcg 7201 accg //