LOCUS       Exported               10840 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses AXL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLentiCMVNeoDEST(705-1)-hAXL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Axelrod HD, Valkenburg KC, Amend SR, Hicks JL, Parsana P, Torga G, 
            DeMarzo AM, Pienta KJ
  TITLE     AXL Is a Putative Tumor Suppressor and Dormancy Regulator in 
            Prostate Cancer.
  JOURNAL   Mol Cancer Res. 2019 Feb;17(2):356-369. doi: 
            10.1158/1541-7786.MCR-18-0718. Epub 2018 Oct 5.
  PUBMED    30291220
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             106..966
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2049..2079
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4134..4437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4438..4641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4592..4612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4638..4662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4746..4770
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7459..7483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7514..8102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7567..7587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7985..7996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8145..8644
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8180..8203)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8577..8596
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8658..9461
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8721..8740)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9331..9350
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9552..9785
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9857..9978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9894..9913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9948..9967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10018..10153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10080..10099
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10173..10192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10174..10192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10202..10219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10202..10218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10211..10233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10360..10815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10447..10466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10657..10678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca
     4141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4501 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4561 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4621 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     4681 cgctgttttg acctccatag aagacaccga ctctagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggca ccatggcgtg gcggtgcccc aggatgggca
     4801 gggtcccgct ggcctggtgc ttggcgctgt gcggctgggc gtgcatggcc cccaggggca
     4861 cgcaggctga agaaagtccc ttcgtgggca acccagggaa tatcacaggt gcccggggac
     4921 tcacgggcac ccttcggtgt cagctccagg ttcagggaga gccccccgag gtacattggc
     4981 ttcgggatgg acagatcctg gagctcgcgg acagcaccca gacccaggtg cccctgggtg
     5041 aggatgaaca ggatgactgg atagtggtca gccagctcag aatcacctcc ctgcagcttt
     5101 ccgacacggg acagtaccag tgtttggtgt ttctgggaca tcagaccttc gtgtcccagc
     5161 ctggctatgt tgggctggag ggcttgcctt acttcctgga ggagcccgaa gacaggactg
     5221 tggccgccaa cacccccttc aacctgagct gccaagctca gggaccccca gagcccgtgg
     5281 acctactctg gctccaggat gctgtccccc tggccacggc tccaggtcac ggcccccagc
     5341 gcagcctgca tgttccaggg ctgaacaaga catcctcttt ctcctgcgaa gcccataacg
     5401 ccaagggggt caccacatcc cgcacagcca ccatcacagt gctcccccag cagccccgta
     5461 acctccacct ggtctcccgc caacccacgg agctggaggt ggcttggact ccaggcctga
     5521 gcggcatcta ccccctgacc cactgcaccc tgcaggctgt gctgtcagac gatgggatgg
     5581 gcatccaggc gggagaacca gaccccccag aggagcccct cacctcgcaa gcatccgtgc
     5641 ccccccatca gcttcggcta ggcagcctcc atcctcacac cccttatcac atccgcgtgg
     5701 catgcaccag cagccagggc ccctcatcct ggacccactg gcttcctgtg gagacgccgg
     5761 agggagtgcc cctgggcccc cctgagaaca ttagtgctac gcggaatggg agccaggcct
     5821 tcgtgcattg gcaagagccc cgggcgcccc tgcagggtac cctgttaggg taccggctgg
     5881 cgtatcaagg ccaggacacc ccagaggtgc taatggacat agggctaagg caagaggtga
     5941 ccctggagct gcagggggac gggtctgtgt ccaatctgac agtgtgtgtg gcagcctaca
     6001 ctgctgctgg ggatggaccc tggagcctcc cagtacccct ggaggcctgg cgcccagggc
     6061 aagcacagcc agtccaccag ctggtgaagg aaccttcaac tcctgccttc tcgtggccct
     6121 ggtggtatgt actgctagga gcagtcgtgg ccgctgcctg tgtcctcatc ttggctctct
     6181 tccttgtcca ccggcgaaag aaggagaccc gttatggaga agtgtttgaa ccaacagtgg
     6241 aaagaggtga actggtagtc aggtaccgcg tgcgcaagtc ctacagtcgt cggaccactg
     6301 aagctacctt gaacagcttg ggcatcagtg aagagctgaa ggagaagctg cgggatgtga
     6361 tggtggaccg gcacaaggtg gccctgggga agactctggg agagggagag tttggagctg
     6421 tgatggaagg ccagctcaac caggacgact ccatcctcaa ggtggctgtg aagacgatga
     6481 agattgccat ctgcacgagg tcagagctgg aggatttcct gagtgaagcg gtctgcatga
     6541 aggaatttga ccatcccaac gtcatgaggc tcatcggtgt ctgtttccag ggttctgaac
     6601 gagagagctt cccagcacct gtggtcatct tacctttcat gaaacatgga gacctacaca
     6661 gcttcctcct ctattcccgg ctcggggacc agccagtgta cctgcccact cagatgctag
     6721 tgaagttcat ggcagacatc gccagtggca tggagtatct gagtaccaag agattcatac
     6781 accgggacct ggcggccagg aactgcatgc tgaatgagaa catgtccgtg tgtgtggcgg
     6841 acttcgggct ctccaagaag atctacaatg gggactacta ccgccaggga cgtatcgcca
     6901 agatgccagt caagtggatt gccattgaga gtctagctga ccgtgtctac accagcaaga
     6961 gcgatgtgtg gtccttcggg gtgacaatgt gggagattgc cacaagaggc caaaccccat
     7021 atccgggcgt ggagaacagc gagatttatg actatctgcg ccagggaaat cgcctgaagc
     7081 agcctgcgga ctgtctggat ggactgtatg ccttgatgtc gcggtgctgg gagctaaatc
     7141 cccaggaccg gccaagtttt acagagctgc gggaagattt ggagaacaca ctgaaggcct
     7201 tgcctcctgc ccaggagcct gacgaaatcc tctatgtcaa catggatgag ggtggaggtt
     7261 atcctgaacc ccctggagct gcaggaggag ctgacccccc aacccagcca gaccctaagg
     7321 attcctgtag ctgcctcact gcggctgagg tccatcctgc tggacgctat gtcctctgcc
     7381 cttccacaac ccctagcccc gctcagcctg ctgatagggg ctccccagca gccccagggc
     7441 aggaggatgg tgcctagaac ccagctttct tgtacaaagt ggttgatatc cagcacagtg
     7501 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7561 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7621 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7681 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7741 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7801 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7861 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7921 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7981 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8041 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     8101 gcctggaatt ctgcagatat ccggactagt gatctaattc taccgggtag gggaggcgct
     8161 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     8221 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     8281 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     8341 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     8401 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     8461 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     8521 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     8581 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     8641 ttcgacctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
     8701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     8761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     8821 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     8881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     9001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     9061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     9121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     9181 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     9241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     9301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     9361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     9421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatccg tcgagggccc
     9481 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9541 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     9601 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9661 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9721 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9781 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9841 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9901 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9961 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
    10021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    10081 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    10141 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
    10201 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10261 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10321 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    10381 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    10441 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    10501 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    10561 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    10621 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    10681 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    10741 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    10801 taacgcttac aatttaggtg gcacttttcg gggaaatgtg
//
