LOCUS Exported 11522 bp ds-DNA circular SYN 17-JAN-2024 DEFINITION expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon. ACCESSION . VERSION . KEYWORDS T7-VEE-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11522) AUTHORS Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF TITLE Efficient generation of human iPSCs by a synthetic self-replicative RNA. JOURNAL Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. PUBMED 23910086 REFERENCE 2 (bases 1 to 11522) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2024 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11522 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 1535..1543 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" regulatory 7600..7609 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7606..8322 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7651..7672) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7912..7931) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8259..8280 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(8554..8571) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 8781..8800 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8962..9561 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8962..8981) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9458..9478 /label=Puro-F /note="Puromycin resistance gene, forward primer" promoter 9760..9864 /gene="bla" /label=AmpR promoter CDS 9865..10725 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10083..10102) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10896..11484 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11385..11404 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 11505..11522 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg 61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg 121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc 181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa 241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat 301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg 361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc 421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc 481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag 541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta 601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa 661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt 721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga 781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact 841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg 901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta 961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg 1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac 1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta 1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg 1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa 1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc 1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg 1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa 1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg 1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt 1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaggcagacg 1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa 1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg 1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga 1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg 1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca 1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag 1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg 2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag 2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa 2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag 2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga 2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg 2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata 2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac 2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc 2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc 2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa 2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc 2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca 2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg 2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg 2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga 2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag 3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc 3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca 3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact 3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg 3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc 3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc 3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc 3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag 3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg 3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt 3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg 3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc 3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc 3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa 3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct 3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc 3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg 4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag 4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc 4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac 4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt 4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca 4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga 4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg 4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg 4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg 4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca 4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg 4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca 4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg 4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa 4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat 4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct 4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag 5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac 5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg 5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg 5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat 5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca 5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc 5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa 5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc 5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc 5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga 5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg 5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa 5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc 5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta 5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta 5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc 6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg 6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta 6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca 6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac 6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag 6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg 6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt 6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa 6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca 6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa 6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag 6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga 6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact 6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg 6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt 6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta 6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag 7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg 7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag 7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga 7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc 7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg 7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg 7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca 7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag 7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa 7561 gtctagcata tgggcgcgcc ctcagcatcg attcaattcg ccaccatggt gagcaagggc 7621 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 7681 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 7741 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 7801 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 7861 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 7921 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 7981 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 8041 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 8101 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 8161 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 8221 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 8281 accgccgccg ggatcactct cggcatggac gagctgtaca agtagtctag agtcgacccg 8341 ggcggccgca actaacttaa gctagcaacg gtttccctct agcgggatca attccgcccc 8401 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 8461 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 8521 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 8581 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 8641 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 8701 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 8761 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 8821 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 8881 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 8941 tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct cgccacccgc 9001 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 9061 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 9121 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 9181 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 9241 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 9301 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 9361 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 9421 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 9481 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 9541 acccgcaagc ccggtgcctg agaattggca agctgcttac atagaactcg cggcgattgg 9601 catgccgcct taaaattttt attttatttt ttcttttctt ttccgaatcg gattttgttt 9661 ttaatatttc aaaaaaaaaa aaaaaaaaaa aaaaaacgcg tcgaggggaa ttaattcttg 9721 aagacgaaag ggccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 9781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 9841 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 9901 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 9961 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 10021 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 10081 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 10141 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 10201 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 10261 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 10321 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 10381 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 10441 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 10501 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 10561 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 10621 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 10681 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 10741 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 10801 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 10861 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 10921 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 10981 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 11041 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 11101 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 11161 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 11221 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 11281 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 11341 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 11401 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 11461 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcga gctctaatac gactcactat 11521 ag //