LOCUS       Exported                3801 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSXneo 135(T2AG3)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Hanish JP, Yanowitz JL, de Lange T
  TITLE     Stringent sequence requirements for the formation of human 
            telomeres.
  JOURNAL   Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5.
  PUBMED    8090736
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             472..1275
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(535..554)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1145..1164
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1282..1330
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(1381..1400)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1382..1400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1487..1504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1658..2246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1738..1757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2417..3277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3040..3059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3278..3382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3450..3468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3506..3528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3628..3647
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3728..3746
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3728..3745
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta
       61 accctaaccc caaccctaac cctaacccta acgctaaccc taaccccaac cctaacccca
      121 accccaaccc caaccccaac cccaaccgga attccgcgga attcgagctc ggtacccggg
      181 gatcctctag agtcgagcag tgtggttttc aagaggaagc aaaaagcctc tccacccagg
      241 cctggaatgt ttccacccaa tgtcgagcag tgtggttttg caagaggaag caaaaagcct
      301 ctccacccag gcctggaatg tttccaccca atgtcgagca aaccccgccc agcgtcttgt
      361 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc
      421 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagccaa tatgggatcg
      481 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
      541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
      601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
      661 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
      721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
      781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
      841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
      901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
      961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1021 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     1201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1261 gacgagttct tctgagggga tcggcaataa aaagacagaa taaaacgcac gggtgttggg
     1321 tcgtttgttc ggatccgtcg agcagctgaa gcttgcatgc ctgcaggtcg aggccggtct
     1381 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     1441 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1501 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1561 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1621 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1741 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1801 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1861 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1921 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1981 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2041 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2101 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2161 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2221 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2281 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2341 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2401 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2461 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2521 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2581 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2641 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2701 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2761 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2821 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2881 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2941 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3001 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3061 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3121 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3181 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3241 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3301 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3421 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3481 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3541 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3601 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     3661 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     3721 acatacgatt taggtgacac tatagaacca gatctatcga tgcggccgca attccgaacc
     3781 ctaaccctaa ccctaaccct a
//
