LOCUS Exported 11759 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of PDGFRA V561D. ACCESSION . VERSION . KEYWORDS pHAGE-PDGFRA-V561D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11759) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11759) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11759 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6719..6743) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 6751..6769 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6794..6813) /label=T7 /note="T7 promoter, forward primer" promoter complement(6795..6813) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(6819..6839) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6893..6934 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6955..7522 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6972..7524 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7116..7133) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7343..7362 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7536..8135 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7536..7555) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8032..8052 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8149..8737 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8202..8222) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8620..8631) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8812..9045 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9164..9182) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9250..9354 /gene="bla" /label=AmpR promoter CDS 9355..10215 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9573..9592) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10386..10974 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10875..10894 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11128..11145 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11237..11256 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11385..11406 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11421..11451 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11459..11475 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11464..11486 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11483..11499 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11483..11499 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11549..11568 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gggacttccc atccggcgtt cctggtctta 3481 ggctgtcttc tcacagggct gagcctaatc ctctgccagc tttcattacc ctctatcctt 3541 ccaaatgaaa atgaaaaggt tgtgcagctg aattcatcct tttctctgag atgctttggg 3601 gagagtgaag tgagctggca gtaccccatg tctgaagaag agagctccga tgtggaaatc 3661 agaaatgaag aaaacaacag cggccttttt gtgacggtct tggaagtgag cagtgcctcg 3721 gcggcccaca cagggttgta cacttgctat tacaaccaca ctcagacaga agagaatgag 3781 cttgaaggca ggcacattta catctatgtg ccagacccag atgtagcctt tgtacctcta 3841 ggaatgacgg attatttagt catcgtggag gatgatgatt ctgccattat accttgtcgc 3901 acaactgatc ccgagactcc tgtaacctta cacaacagtg agggggtggt acctgcctcc 3961 tacgacagca gacagggctt taatgggacc ttcactgtag ggccctatat ctgtgaggcc 4021 accgtcaaag gaaagaagtt ccagaccatc ccatttaatg tttatgcttt aaaagcaaca 4081 tcagagctgg atctagaaat ggaagctctt aaaaccgtgt ataagtcagg ggaaacgatt 4141 gtggtcacct gtgctgtttt taacaatgag gtggttgacc ttcaatggac ttaccctgga 4201 gaagtgaaag gcaaaggcat cacaatgctg gaagaaatca aagtcccatc catcaaattg 4261 gtgtacactt tgacggtccc cgaggccacg gtgaaagaca gtggagatta cgaatgtgct 4321 gcccgccagg ctaccaggga ggtcaaagaa atgaagaaag tcactatttc tgtccatgag 4381 aaaggtttca ttgaaatcaa acccaccttc agccagttgg aagctgtcaa cctgcatgaa 4441 gtcaaacatt ttgttgtaga ggtgcgggcc tacccacctc ccaggatatc ctggctgaaa 4501 aacaatctga ctctgattga aaatctcact gagatcacca ctgatgtgga aaagattcag 4561 gaaataaggt atcgaagcaa attaaagctg atccgtgcta aggaagaaga cagtggccat 4621 tatactattg tagctcaaaa tgaagatgct gtgaagagct atacttttga actgttaact 4681 caagttcctt catccattct ggacttggtc gatgatcacc atggctcaac tgggggacag 4741 acggtgaggt gcacagctga aggcacgccg cttcctgata ttgagtggat gatatgcaaa 4801 gatattaaga aatgtaataa tgaaacttcc tggactattt tggccaacaa tgtctcaaac 4861 atcatcacgg agatccactc ccgagacagg agtaccgtgg agggccgtgt gactttcgcc 4921 aaagtggagg agaccatcgc cgtgcgatgc ctggctaaga atctccttgg agctgagaac 4981 cgagagctga agctggtggc tcccaccctg cgttctgaac tcacggtggc tgctgcagtc 5041 ctggtgctgt tggtgattgt gatcatctca cttattgtcc tggttgtcat ttggaaacag 5101 aaaccgaggt atgaaattcg ctggagggac attgaatcaa tcagcccaga tggacatgaa 5161 tatatttatg tggacccgat gcagctgcct tatgactcaa gatgggagtt tccaagagat 5221 ggactagtgc ttggtcgggt cttggggtct ggagcgtttg ggaaggtggt tgaaggaaca 5281 gcctatggat taagccggtc ccaacctgtc atgaaagttg cagtgaagat gctaaaaccc 5341 acggccagat ccagtgaaaa acaagctctc atgtctgaac tgaagataat gactcacctg 5401 gggccacatt tgaacattgt aaacttgctg ggagcctgca ccaagtcagg ccccatttac 5461 atcatcacag agtattgctt ctatggagat ttggtcaact atttgcataa gaatagggat 5521 agcttcctga gccaccaccc agagaagcca aagaaagagc tggatatctt tggattgaac 5581 cctgctgatg aaagcacacg gagctatgtt attttatctt ttgaaaacaa tggtgactac 5641 atggacatga agcaggctga tactacacag tatgtcccca tgctagaaag gaaagaggtt 5701 tctaaatatt ccgacatcca gagatcactc tatgatcgtc cagcctcata taagaagaaa 5761 tctatgttag actcagaagt caaaaacctc ctttcagatg ataactcaga aggccttact 5821 ttattggatt tgttgagctt cacctatcaa gttgcccgag gaatggagtt tttggcttca 5881 aaaaattgtg tccaccgtga tctggctgct cgcaacgtcc tcctggcaca aggaaaaatt 5941 gtgaagatct gtgactttgg cctggccaga gacatcatgc atgattcgaa ctatgtgtcg 6001 aaaggcagta cctttctgcc cgtgaagtgg atggctcctg agagcatctt tgacaacctc 6061 tacaccacac tgagtgatgt ctggtcttat ggcattctgc tctgggagat cttttccctt 6121 ggtggcaccc cttaccccgg catgatggtg gattctactt tctacaataa gatcaagagt 6181 gggtaccgga tggccaagcc tgaccacgct accagtgaag tctacgagat catggtgaaa 6241 tgctggaaca gtgagccgga gaagagaccc tccttttacc acctgagtga gattgtggag 6301 aatctgctgc ctggacaata taaaaagagt tatgaaaaaa ttcacctgga cttcctgaag 6361 agtgaccatc ctgctgtggc acgcatgcgt gtggactcag acaatgcata cattggtgtc 6421 acctacaaaa acgaggaaga caagctgaag gactgggagg gtggtctgga tgagcagaga 6481 ctgagcgctg acagtggcta catcattcct ctgcctgaca ttgaccctgt ccctgaggag 6541 gaggacctgg gcaagaggaa cagacacagc tcgcagacct ctgaagagag tgccattgag 6601 acgggttcca gcagttccac cttcatcaag agagaggacg agaccattga agacatcgac 6661 atgatggacg acatcggcat agactcttca gacctggtgg aagacagctt cctgtagaac 6721 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt gtagtatgaa 6781 gaataatgaa atgccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt 6841 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc 6901 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc 6961 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta 7021 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc 7081 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct 7141 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt 7201 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa 7261 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg 7321 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga 7381 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac 7441 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt 7501 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc 7561 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 7621 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 7681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 7741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 7801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 7861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 7921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 7981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 8041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 8101 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg 8161 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 8221 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 8281 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 8341 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 8401 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 8461 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 8521 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 8581 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 8641 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 8701 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga 8761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 8821 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 8881 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8941 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 9001 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 9061 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg 9121 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta 9181 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 9241 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 9301 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 9361 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 9421 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 9481 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 9541 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 9601 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 9661 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 9721 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 9781 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 9841 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 9901 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 9961 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 10021 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 10081 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 10141 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 10201 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 10261 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 10321 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 10381 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 10441 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 10501 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 10561 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 10621 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 10681 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 10741 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 10801 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 10861 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 10921 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 10981 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 11041 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 11101 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 11161 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg 11221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 11281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac 11341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 11401 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 11461 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat 11521 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 11581 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 11641 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 11701 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt //