LOCUS Exported 2423 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-PaccD (-82 to +23) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2423) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2423) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2423 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(629..646) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(739..1327) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(819..838) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1501..2295) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2296..2400) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcaggag gacataggag aagccgccct ttattaaaaa 421 ttatattatt ttaaataata taaagggggt tccaacatat taatatatag tgaagtgttc 481 ccccagattc agaactactt gagaccggta ccctctgggc ctcatgggcc ttcctttcac 541 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt 601 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 661 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa 721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 1021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 1081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 1141 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 1201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 1261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 1321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 1381 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 1441 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 1501 ttagaaaaat tcatccagca gacgataaaa cgcaatacgc tggctatccg gtgccgcaat 1561 gccatacagc accagaaaac gatccgccca ttcgccgccc agttcttccg caatatcacg 1621 ggtggccagc gcaatatcct gataacgatc cgccacgccc agacggccgc aatcaataaa 1681 gccgctaaaa cggccatttt ccaccataat gttcggcagg cacgcatcac catgggtcac 1741 caccagatct tcgccatccg gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc 1801 caggccctga tgttcttcat ccagatcatc ctgatccacc aggcccgctt ccatacgggt 1861 acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag 1921 ggtatgcaga cgacgcatgg catccgccat aatgctcact ttttctgccg gcgccagatg 1981 gctagacagc agatcctgac ccggcacttc gcccagcagc agccaatcac ggcccgcttc 2041 ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg gtggccagcc agctcagacg 2101 cgccgcttca tcctgcagct cgttcagcgc accgctcaga tcggttttca caaacagcac 2161 cggacgaccc tgcgcgctca gacgaaacac cgccgcatca gagcagccaa tggtctgctg 2221 cgcccaatca tagccaaaca gacgttccac ccacgctgcc gggctacccg catgcaggcc 2281 atcctgttca atcatactct tcctttttca atattattga agcatttatc agggttattg 2341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 2401 cacatttccc cgaaaagtgc cac //