LOCUS Exported 2410 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-PatpB (NEP functional PEP disabled) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2410) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2410) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2410 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(616..633) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(726..1314) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(806..825) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1488..2282) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2283..2387) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggcctaggc gcgccatgag ctcggtctca ggagaataga aatagaaaat aaagttcagg 421 ttcgaattcc atagaataga taatatggat gggattgtcg cgaattgata gacaatactt 481 gagaccggta cctcttaatt aactggcctc atgggccttc ctttcactgc ccgctttcca 541 gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg 601 ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg 661 ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttatta gaaaaattca 1501 tccagcagac gataaaacgc aatacgctgg ctatccggtg ccgcaatgcc atacagcacc 1561 agaaaacgat ccgcccattc gccgcccagt tcttccgcaa tatcacgggt ggccagcgca 1621 atatcctgat aacgatccgc cacgcccaga cggccgcaat caataaagcc gctaaaacgg 1681 ccattttcca ccataatgtt cggcaggcac gcatcaccat gggtcaccac cagatcttcg 1741 ccatccggca tgctcgcttt cagacgcgca aacagctctg ccggtgccag gccctgatgt 1801 tcttcatcca gatcatcctg atccaccagg cccgcttcca tacgggtacg cgcacgttca 1861 atacgatgtt tcgcctgatg atcaaacgga caggtcgccg ggtccagggt atgcagacga 1921 cgcatggcat ccgccataat gctcactttt tctgccggcg ccagatggct agacagcaga 1981 tcctgacccg gcacttcgcc cagcagcagc caatcacggc ccgcttcggt caccacatcc 2041 agcaccgccg cacacggaac accggtggtg gccagccagc tcagacgcgc cgcttcatcc 2101 tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa acagcaccgg acgaccctgc 2161 gcgctcagac gaaacaccgc cgcatcagag cagccaatgg tctgctgcgc ccaatcatag 2221 ccaaacagac gttccaccca cgctgccggg ctacccgcat gcaggccatc ctgttcaatc 2281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 2341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 2401 aaagtgccac //