LOCUS Exported 2367 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-PatpB (NEP) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2367) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2367) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2367 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(573..590) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(683..1271) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(763..782) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1445..2239) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2240..2344) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcaggag aatagaaata gaaaataaag ttcaggttcg 421 aattccatag aatagataat acttgagacc ggtaccctct gggcctcatg ggccttcctt 481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc 541 tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc 601 gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta 661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 1021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 1081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 1141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 1201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 1261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 1321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 1381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 1441 gttattagaa aaattcatcc agcagacgat aaaacgcaat acgctggcta tccggtgccg 1501 caatgccata cagcaccaga aaacgatccg cccattcgcc gcccagttct tccgcaatat 1561 cacgggtggc cagcgcaata tcctgataac gatccgccac gcccagacgg ccgcaatcaa 1621 taaagccgct aaaacggcca ttttccacca taatgttcgg caggcacgca tcaccatggg 1681 tcaccaccag atcttcgcca tccggcatgc tcgctttcag acgcgcaaac agctctgccg 1741 gtgccaggcc ctgatgttct tcatccagat catcctgatc caccaggccc gcttccatac 1801 gggtacgcgc acgttcaata cgatgtttcg cctgatgatc aaacggacag gtcgccgggt 1861 ccagggtatg cagacgacgc atggcatccg ccataatgct cactttttct gccggcgcca 1921 gatggctaga cagcagatcc tgacccggca cttcgcccag cagcagccaa tcacggcccg 1981 cttcggtcac cacatccagc accgccgcac acggaacacc ggtggtggcc agccagctca 2041 gacgcgccgc ttcatcctgc agctcgttca gcgcaccgct cagatcggtt ttcacaaaca 2101 gcaccggacg accctgcgcg ctcagacgaa acaccgccgc atcagagcag ccaatggtct 2161 gctgcgccca atcatagcca aacagacgtt ccacccacgc tgccgggcta cccgcatgca 2221 ggccatcctg ttcaatcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2341 cgcgcacatt tccccgaaaa gtgccac //