LOCUS Exported 2398 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-PatpB (PEP and NEP functional) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2398) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2398) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2398 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(604..621) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(714..1302) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(794..813) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1476..2270) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2271..2375) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcaggag aatagaaata gaaaataaag ttcaggttcg 421 aattccatag aatagataat atggatggga ttgtctataa tgatagacaa tacttgagac 481 cggtaccctc tgggcctcat gggccttcct ttcactgccc gctttccagt cgggaaacct 541 gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg cgctctccgc 601 ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg tgcctaatga 661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1441 taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc cagcagacga 1501 taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag aaaacgatcc 1561 gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat atcctgataa 1621 cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc attttccacc 1681 ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc atccggcatg 1741 ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc ttcatccaga 1801 tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat acgatgtttc 1861 gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg catggcatcc 1921 gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc ctgacccggc 1981 acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag caccgccgca 2041 cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg cagctcgttc 2101 agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc gctcagacga 2161 aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc aaacagacgt 2221 tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat actcttcctt 2281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 2341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //