LOCUS Exported 6000 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Ras GTPase, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS tdEos-Rab7a-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6000) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 6000) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6000 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 85..388 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 389..592 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 543..563 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 637..1314 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" CDS 1363..2037 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN ARR" polyA_signal 2812..2933 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2849..2868) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2903..2922 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2940..3395) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3077..3098) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3289..3308 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3422..3526 /gene="bla" /label=AmpR promoter promoter 3528..3885 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3551..3571) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3736..3871 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3798..3817 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3920..4714 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3974..3993) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4584..4603 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4902..4921) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4946..4993 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5322..5910 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5811..5830 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 601 gaaccgtcag atccgctagc gctaccggtc gccaccatga gtgcgattaa gccagacatg 661 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat 721 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga 781 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc 841 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg 901 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca 961 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat 1021 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg 1081 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat 1141 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc 1201 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt 1261 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccggt 1321 agcggtcacg gtactggttc tactggttct ggttcttctg agagtgcgat taagccagac 1381 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga 1441 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc 1501 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta 1561 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat 1621 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata 1681 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc 1741 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat 1801 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc 1861 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca 1921 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag 1981 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc 2041 ggactcagat ctcgagccat gacctctagg aagaaagtgt tgctgaaggt tatcatcctg 2101 ggagattctg gagtcgggaa gacatcactc atgaaccagt atgtgaataa gaaattcagc 2161 aatcagtaca aagccacaat aggagctgac tttctgacca aggaggtgat ggtggatgac 2221 aggctagtca caatgcagat atgggacaca gcaggacagg aacggttcca gtctctcggt 2281 gtggccttct acagaggtgc agactgctgc gttctggtat ttgatgtgac tgcccccaac 2341 acattcaaaa ccctagatag ctggagagat gagtttctca tccaggccag tccccgagat 2401 cctgaaaact tcccatttgt tgtgttggga aacaagattg acctcgaaaa cagacaagtg 2461 gccacaaagc gggcacaggc ctggtgctac agcaaaaaca acattcccta ctttgagacc 2521 agtgccaagg aggccatcaa cgtggagcag gcgttccaga cgattgcacg gaatgcactt 2581 aagcaggaaa cggaggtgga gctgtacaac gaatttcctg aacctatcaa actggacaag 2641 aatgaccggg ccaaggcctc ggcagaaagc tgcagttgct gaggatccac cggatctaga 2701 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 2761 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 2821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 2941 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 3001 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 3061 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 3121 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 3181 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 3241 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 3301 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 3361 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 3421 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 3481 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 3541 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3721 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3781 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 3841 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 3901 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3961 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4021 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4081 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4141 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4201 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4261 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4321 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4381 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 4441 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 4501 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 4561 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 4621 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4681 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4741 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4801 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4861 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 4921 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 4981 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 5041 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 5101 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 5161 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 5221 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 5281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 5341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 5401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 5461 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 5521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg //