LOCUS Exported 4866 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Golgi, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS wtEos-Golgi-4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4866) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 4866) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4866 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 80..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 384..587 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 538..558 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 874..1551 /codon_start=1 /product="green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)" /label=EosFP /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALLLE GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN ARR" polyA_signal 1673..1794 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1710..1729) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1764..1783 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1801..2256) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1938..1959) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2150..2169 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2283..2387 /gene="bla" /label=AmpR promoter promoter 2389..2746 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2412..2432) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2597..2732 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2659..2678 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2781..3575 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2835..2854) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3445..3464 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3763..3782) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3807..3854 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4183..4771 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4672..4691 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc 601 gtcagatccg ctagcatgag gcttcgggag ccgctcctga gcggcagcgc cgcgatgcca 661 ggcgcgtccc tacagcgggc ctgccgcctg ctcgtggccg tctgcgctct gcaccttggc 721 gtcaccctcg tttactacct ggctggccgc gacctgagcc gcctgcccca actggtcgga 781 gtctccacac cgctgcaggg cggctcgaac agtgccgccg ccatcgggca gtcctccggg 841 gagctccgga ccggaggggc caaggatccc gagagtgcga ttaagccaga catgaagatc 901 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca 961 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg 1021 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa 1081 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa 1141 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa 1201 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca 1261 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat 1321 ggagtgctga cgggtgatat taccatggct ttgttgcttg aaggaaatgc ccattaccga 1381 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac 1441 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg 1501 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg 1561 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 1621 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 1681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 1741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt 1801 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 1861 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 1921 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 1981 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 2041 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 2101 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 2161 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 2221 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 2281 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 2341 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa 2401 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 2461 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 2521 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 2581 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 2641 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 2701 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga 2761 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 2821 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 2881 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 2941 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 3001 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 3061 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 3121 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 3181 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 3241 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 3301 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 3361 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 3421 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 3481 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 3541 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 3601 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 3661 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 3721 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac 3781 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt 3841 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 3901 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 3961 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 4021 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 4081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 4141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4861 ggataa //