LOCUS Exported 6059 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Myc-Rab35 WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6059) AUTHORS Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, Fotouhi M, Maret D, Ritter B, Del Maestro RF, McPherson PS TITLE Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. JOURNAL J Cell Sci. 2013 Feb 1;126(Pt 3):722-31. doi: 10.1242/jcs.112375. Epub 2012 Dec 21. PUBMED 23264734 REFERENCE 2 (bases 1 to 6059) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6059 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 83..462 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 463..666 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 617..637 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 711..730 /label=T7 /note="T7 promoter, forward primer" promoter 711..729 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 743..752 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(1460..1478) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1461..1478) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(1498..1515) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1504..1728 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1774..2202 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1861..1880) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2071..2092 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2211..2231) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2216..2545 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2396..2531 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2458..2477 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2612..3406 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2666..2685) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3276..3295 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3580..3701 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3617..3636) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3671..3690 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3750..3766) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3750..3766) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3763..3785) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3774..3790 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3798..3828) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3843..3864 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3981..3998) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4152..4740) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4232..4251) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4911..5771) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5534..5553 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5772..5876) /gene="bla" /label=AmpR promoter primer_bind complement(5951..5970) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat 61 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt 121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 661 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact 721 cactataggg agacccaagc ttgccaccat ggcatcaatg cagaagctga tctcagagga 781 ggacctggga tccatggccc gggactacga ccacctcttc aagctgctca tcatcggcga 841 cagcggtgtg ggcaagagca gtttactgtt gcgttttgca gacaacactt tctcaggcag 901 ctacatcacc acgatcggag tggatttcaa gatccggacc gtggagatca acggggagaa 961 ggtgaagctg cagatctggg acacagcggg gcaggagcgc ttccgcacca tcacctccac 1021 gtattatcgg gggacccacg gggtcattgt ggtttacgac gtcaccagtg ccgagtcctt 1081 tgtcaacgtc aagcggtggc ttcacgaaat caaccagaac tgtgatgatg tgtgccgaat 1141 attagtgggt aataagaatg acgaccctga gcggaaggtg gtggagacgg aagatgccta 1201 caaattcgcc gggcagatgg gcatccagtt gttcgagacc agcgccaagg agaatgtcaa 1261 cgtggaagag atgttcaact gcatcacgga gctggtcctc cgagcaaaga aagacaacct 1321 ggcaaaacag cagcagcaac aacagaacga tgtggtgaag ctcacgaaga acagtaaacg 1381 aaagaaacgc tgctgctaag aattctgcag atatccatca cactggcggc cgctcgagca 1441 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct 1501 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1561 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1621 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1681 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1741 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1801 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1861 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1921 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1981 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 2041 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 2101 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 2161 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 2221 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 2281 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 2341 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 2401 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 2461 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 2521 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 2581 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2641 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2701 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2761 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 2821 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2881 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 2941 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3001 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3061 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3121 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3181 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 3241 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 3301 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 3361 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3421 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 3481 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3541 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat 3601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 3661 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg 3721 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 3781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 3841 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 3901 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 3961 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 4021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 4081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 4141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 4201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 4261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 4321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 4381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 4441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 4501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 4561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 4621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 4681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 4741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 4801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 4861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 4921 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 4981 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 5041 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 5101 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 5161 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 5221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 5281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 5341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 5401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 5461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 5521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 5581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 5641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 5701 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 5761 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 5821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 5881 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt 5941 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 6001 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggc //