LOCUS Exported 2393 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-mPrrn (5-fold decrease transcription) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2393) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2393) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2393 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(599..616) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(709..1297) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(789..808) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1471..2265) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2266..2370) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatg aagggcgcgc cagagctcgg tctcaggaga ttgacgtgag ggggcaggga 421 tggctatatt tctgggagcg aactccgggc gaattacttg agaccggtac cattaattaa 481 caacctgggc ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt 541 gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct 601 cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa 661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 1081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 1141 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 1201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1441 tatatatgag taaacttggt ctgacagtta ttagaaaaat tcatccagca gacgataaaa 1501 cgcaatacgc tggctatccg gtgccgcaat gccatacagc accagaaaac gatccgccca 1561 ttcgccgccc agttcttccg caatatcacg ggtggccagc gcaatatcct gataacgatc 1621 cgccacgccc agacggccgc aatcaataaa gccgctaaaa cggccatttt ccaccataat 1681 gttcggcagg cacgcatcac catgggtcac caccagatct tcgccatccg gcatgctcgc 1741 tttcagacgc gcaaacagct ctgccggtgc caggccctga tgttcttcat ccagatcatc 1801 ctgatccacc aggcccgctt ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg 1861 atgatcaaac ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg catccgccat 1921 aatgctcact ttttctgccg gcgccagatg gctagacagc agatcctgac ccggcacttc 1981 gcccagcagc agccaatcac ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg 2041 aacaccggtg gtggccagcc agctcagacg cgccgcttca tcctgcagct cgttcagcgc 2101 accgctcaga tcggttttca caaacagcac cggacgaccc tgcgcgctca gacgaaacac 2161 cgccgcatca gagcagccaa tggtctgctg cgcccaatca tagccaaaca gacgttccac 2221 ccacgctgcc gggctacccg catgcaggcc atcctgttca atcatactct tcctttttca 2281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac //