LOCUS Exported 2448 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-PrbcL (-110 to +9) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2448) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2448) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2448 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(654..671) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(764..1352) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(844..863) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1526..2320) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2321..2425) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggcctaggc gcgccatgag ctcggtctca ggagggggga agttcttatt atttaggtta 421 gtcaggtatt tccatttcaa aaaaaaaaaa agtaaaaaag aaaaattggg ttgcgctata 481 tatatgaaag agtatacaat aatgatgtat ttgtacttga gaccggtacc tcttaattaa 541 ctggcctcat gggccttcct ttcactgccc gctttccagt cgggaaacct gtcgtgccag 601 ctgcattaac atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc 661 actgactcgc tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc 721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 1021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 1081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 1141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 1201 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 1261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 1321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 1381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 1441 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 1501 atgagtaaac ttggtctgac agttattaga aaaattcatc cagcagacga taaaacgcaa 1561 tacgctggct atccggtgcc gcaatgccat acagcaccag aaaacgatcc gcccattcgc 1621 cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat atcctgataa cgatccgcca 1681 cgcccagacg gccgcaatca ataaagccgc taaaacggcc attttccacc ataatgttcg 1741 gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc atccggcatg ctcgctttca 1801 gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc ttcatccaga tcatcctgat 1861 ccaccaggcc cgcttccata cgggtacgcg cacgttcaat acgatgtttc gcctgatgat 1921 caaacggaca ggtcgccggg tccagggtat gcagacgacg catggcatcc gccataatgc 1981 tcactttttc tgccggcgcc agatggctag acagcagatc ctgacccggc acttcgccca 2041 gcagcagcca atcacggccc gcttcggtca ccacatccag caccgccgca cacggaacac 2101 cggtggtggc cagccagctc agacgcgccg cttcatcctg cagctcgttc agcgcaccgc 2161 tcagatcggt tttcacaaac agcaccggac gaccctgcgc gctcagacga aacaccgccg 2221 catcagagca gccaatggtc tgctgcgccc aatcatagcc aaacagacgt tccacccacg 2281 ctgccgggct acccgcatgc aggccatcct gttcaatcat actcttcctt tttcaatatt 2341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 2401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac //