LOCUS Exported 11983 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression OptoTGFBRs for transient or stable constitutive expression . ACCESSION . VERSION . KEYWORDS pLNCX2-OptoTGFBRs SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11983) AUTHORS Li Y, Lee M, Kim N, Wu G, Deng D, Kim JM, Liu X, Heo WD, Zi Z TITLE Spatiotemporal Control of TGF-beta Signaling with Light. JOURNAL ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 10.1021/acssynbio.7b00225. Epub 2018 Jan 12. PUBMED 29241005 REFERENCE 2 (bases 1 to 11983) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11983 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 414..589 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 652..851 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1052..1468 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1398..1420 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1436..1452 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 1512..2306 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(1566..1585) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2176..2195 /label=Neo-F /note="Neomycin resistance gene, forward primer" enhancer 2377..2680 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2681..2884 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2835..2855 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 2911..2920 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4621..4677 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" regulatory 4684..4693 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 7366..7375 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7372..8802 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" LTR 8883..9476 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(9579..9601) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 9687..9829 /label=bom /note="basis of mobility region from pBR322" primer_bind 9742..9761 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(9844..9861) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10015..10603) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10095..10114) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10774..11634) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11397..11416 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11635..11739) /gene="bla" /label=AmpR promoter primer_bind 11807..11825 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatgag 1501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 1561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 1621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 1681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 1741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 1801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 1861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 1921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 1981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 2041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 2101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 2161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 2221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 2281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaatagt aatcaattac 2341 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 2401 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 2461 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 2521 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 2581 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 2641 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 2701 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 2761 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 2821 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 2881 agctggttta gtgaaccgtc agatccgcta gccaccatgg gatgtataaa atcaaaaggg 2941 aaagacagcg cgggagcaga tagtgctggt agtgctggta gtgctgggaa ttcgtgccat 3001 aaccgcactg tcattcacca ccgtgtgcca aatgaagagg atccatcact agatcgccct 3061 ttcatttcag agggcaccac cttaaaagat ttaatttatg atatgacaac atcagggtct 3121 ggatcaggtt taccactgct tgttcaaaga acaattgcca ggaccattgt gttacaagaa 3181 agcattggca aaggtcggtt tggagaagtt tggcggggca aatggcgggg agaagaagtt 3241 gctgtgaaga tattctcttc tagagaagag cgttcatggt tccgagaggc agagatttat 3301 cagactgtaa tgttacgcca tgaaaatatc ctgggattta tagcagcaga caacaaagac 3361 aatgggacat ggacgcagct gtggttggtg tcagattatc atgagcatgg atcccttttc 3421 gattacttga atagatacac tgttactgtg gaaggaatga tcaagcttgc tctgtccaca 3481 gcaagtggtc ttgcccatct tcacatggag attgttggta cccaaggaaa accagctatt 3541 gcccatagag atttgaaatc aaagaatatc ttggtgaaga aaaatggaac ctgttgtatt 3601 gcagacttgg gacttgctgt gagacatgat tctgccacag atacaattga tattgctcca 3661 aaccacagag taggcactaa aaggtacatg gcccctgaag ttctagatga ttccataaat 3721 atgaaacatt ttgaatcctt caaacgcgct gacatctatg caatgggctt agtgttctgg 3781 gaaattgctc gacgctgttc tattggtgga atccatgaag actatcagtt gccttattat 3841 gatcttgtac cttctgaccc atcggttgaa gaaatgagaa aagtagtttg cgaacagaag 3901 ttaaggccaa atattccaaa cagatggcag agctgtgagg ccttgagagt gatggctaaa 3961 attatgagag aatgctggta tgccaatgga gcagcaaggc tgacagcttt gcgaattaaa 4021 aaaacattgt cacaactcag ccaacaggaa ggcatcaaaa tgggctcgag ggatccaagt 4081 gctggtggca tgaatggagc tataggaggt gaccttttgc tcaattttcc tgacatgtcg 4141 gtcctagagc gccaaagggc tcacctcaag tacctcaatc ccacctttga ttctcctctc 4201 gccggcttct ttgccgattc ttcaatgatt accggcggcg agatggacag ctatctttcg 4261 actgccggtt tgaatcttcc gatgatgtac ggtgagacga cggtggaagg tgattcaaga 4321 ctctcaattt cgccggaaac gacgcttggg actggaaatt tcaaggcagc gaagtttgat 4381 acagagacta aggattgtaa tgaggcggcg aagaagatga cgatgaacag agatgaccta 4441 gtagaagaag gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa 4501 agcatcaaga agatgaaaca caaagccaag aaagaagaga acaatttctc taatgattca 4561 tctaaagtga cgaaggaatt ggagaaaacg gattatattc atgtaccggt gggctccggc 4621 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccggga 4681 ctagccacca tggggaattc gcgtgtccac cggcagcaga agctgagccc gtcctgggag 4741 agcagcaagc cccggaaact gatggatttc agtgacaatt gtgccatcat cctggaggac 4801 gaccgctccg acatcagctc cacgtgcgcc aacaacatca accacaacac ggagctgctg 4861 cccatcgagc tggacacgct ggtggggaag ggccgcttcg ccgaggtcta caaggccaag 4921 ctgaagcaga acacctcaga gcagtttgag accgtggctg tcaagatctt cccctacgag 4981 gagtactcct cgtggaaaac agagaaggac atcttctccg atatcaacct gaagcatgag 5041 aacatcctgc agttcctgac ggccgaggag cggaagacag agctgggcaa gcagtactgg 5101 ctgatcacgg cgttccacgc gaagggcaac ctgcaggagt acctcacgag gcatgtcatc 5161 agctgggagg acctgaggaa gctgggcagc tccctggccc ggggcatcgc tcatctccac 5221 agtgaccaca ctccttgtgg gaggcccaag atgcccattg ttcacaggga cctcaagagc 5281 tctaacatcc tagtgaagaa cgacttgacc tgttgcctgt gtgacttcgg gctgtccttg 5341 cgcctggacc ctactctgtc tgtggatgac ctggccaaca gcgggcaggt gggaacggca 5401 agatacatgg ccccggaagt tctagaatcc aggatgaatc tggaaaacgt ggagtcgttc 5461 aagcagacgg atgtctactc catggctctg gtactctggg aaatgacgtc ccgctgcaat 5521 gctgtgggag aagtgaagga ttacgagccc ccatttggtt ccaaggtgcg ggagcacccc 5581 tgtgtggaga gcatgaaaga cagtgtgctg agagaccgag ggcggccgga aattcccagc 5641 ttctggctca accaccaggg catccagatc gtgtgtgaga ctttgaccga gtgctgggac 5701 catgaccccg aagcccgtct cacagcacag tgtgtggcag agcgcttcag tgagctggag 5761 catccggaga gactctctgg gaggagctgc tcccaggaga agattccaga agatggctcg 5821 ctgaacacta ccaaagggga tccgacaggt atgaagatgg acaaaaagac catcgtctgg 5881 tttcggagag atttgagaat agaagataat cccgcgctcg ccgccgcggc ccacgagggt 5941 tccgtcttcc ccgttttcat ttggtgtcct gaagaagaag gccagtttta tcccggaagg 6001 gcctctaggt ggtggatgaa gcaaagtctg gcccatctta gccagtcact gaaagcactg 6061 ggcagtgatc ttaccctgat caagacacac aataccatct ctgccatcct cgactgcatc 6121 agggtgaccg gcgcaacgaa agtcgtgttt aaccacctgt acgatccagt tagtctggtg 6181 cgcgaccaca ctgtgaagga gaagctggtg gaacggggga tcagtgtgca gagctacaac 6241 ggggaccttc tgtacgagcc atgggagatc tattgcgaga aagggaaacc gttcacctcc 6301 ttcaacagtt actggaagaa atgtttggat atgtcaatag agtccgttat gttgccccct 6361 ccctggagac tgatgccgat tactgctgct gcagaggcca tctgggcctg ctccatcgag 6421 gaactcggtc tggaaaatga agcagaaaag ccaagcaatg cacttctcac tagagcctgg 6481 agccccggct ggtctaatgc cgacaagctg cttaacgagt tcatcgaaaa acaactgatt 6541 gactacgcga agaactccaa gaaagtggta ggtaactcaa ctagcttgct ctctccatat 6601 ctccattttg gcgagatttc tgtccgccat gtatttcagt gcgctcggat gaaacagatt 6661 atctgggctc gcgataaaaa cagcgaaggc gaagaaagcg ccgatctgtt cctgcgaggg 6721 atcggacttc gggaatactc ccggtatata tgtttcaact ttccattcac acacgagcag 6781 agtctgttgt cccacctcag gttcttcccc tgggacgccg atgtcgacaa attcaaggca 6841 tggagacagg gaaggacagg gtacccactc gtggatgctg gcatgagaga gctctgggct 6901 acaggctgga tgcacaaccg catccgggta atcgtgtcct catttgctgt caagtttctg 6961 ctcctgcctt ggaaatgggg aatgaagtac ttttgggata cccttctcga cgccgacttg 7021 gagtgtgaca ttctgggatg gcaatatatt agcgggtcaa ttcctgacgg ccatgagttg 7081 gacaggttgg acaatccggc cttgcaggga gctaagtatg atcccgaagg agagtatatt 7141 cgacagtggc tccccgagct ggcccgactt cctacggagt ggattcacca tccttgggac 7201 gcaccactga cagtgctcaa ggcaagcggg gtggagctgg gcaccaatta cgctaagcct 7261 atagttgata tagatacagc acgcgagctg ctggctaaag cgatctctcg cactcgggag 7321 gcgcagatta tgatcggtgc tgccggcagc ggcagcccac cggtcgccac catggtgagc 7381 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg 7441 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 7501 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 7561 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac 7621 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 7681 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg 7741 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc 7801 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac 7861 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg 7921 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc 7981 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac 8041 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc 8101 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc 8161 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 8221 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 8281 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc 8341 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 8401 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac 8461 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc 8521 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag 8581 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc 8641 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac 8701 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc 8761 caccacctgt tcctgtacgg catggacgag ctgtacaagt aaagcggccg cttgtcgaca 8821 ggccttaatg gcctaacatc gataaaataa aagattttat ttagtctcca gaaaaagggg 8881 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag 8941 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga 9001 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 9061 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc 9121 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt 9181 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta 9241 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct 9301 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc 9361 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt 9421 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg 9481 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa 9541 gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 9601 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 9661 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 9721 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 9781 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 9841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 9901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 9961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 10021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 10081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 10141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 10201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 10261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 10321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 10381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 10441 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 10501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 10561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 10621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 10681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 10741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 10801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 10861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 10921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 10981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 11041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 11101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 11161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 11221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 11281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 11341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 11401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 11461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 11521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 11581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 11641 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 11701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 11761 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 11821 gaggcccttt cgtcttcaag aattcatacc agatcaccga aaactgtcct ccaaatgtgt 11881 ccccctcaca ctcccaaatt cgcgggcttc tgcctcttag accactctac cctattcccc 11941 acactcaccg gagccaaagc cgcggccctt ccgtttcttt gct //