LOCUS Exported 2468 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-Prrn (N. tabacum) BPrrn19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2468) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2468) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2468 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(674..691) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(784..1372) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(864..883) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1546..2340) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2341..2445) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccacgtg tcttgtccag agctcggtct caggaggctc ccccgccgtc gttcaatgag 421 aatggataag aggctcgtgg gattgacgtg agggggcagg gatggctata cttctgggag 481 cgaactccgg gcgaatacga agcgcttgga tacagttgta gggagggatt ttacttgaga 541 ccggtacctg gagcacaaga ctggcctcat gggccttcct ttcactgccc gctttccagt 601 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg 661 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg 721 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 1261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 1321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 1381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 1441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 1501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc 1561 cagcagacga taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag 1621 aaaacgatcc gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat 1681 atcctgataa cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc 1741 attttccacc ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc 1801 atccggcatg ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc 1861 ttcatccaga tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat 1921 acgatgtttc gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg 1981 catggcatcc gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc 2041 ctgacccggc acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag 2101 caccgccgca cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg 2161 cagctcgttc agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc 2221 gctcagacga aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc 2281 aaacagacgt tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat 2341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 2401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 2461 agtgccac //