LOCUS Exported 2491 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-Zm PclpP::5'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2491) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2491) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2491 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(697..714) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(807..1395) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(887..906) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1569..2363) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2364..2468) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatg aagggcgcgc caggtctcag gagaaagagc tcggatcctc tatgtattaa 421 tagaatctat agtattctta tagaataaga aaaaaaaaat gaagataata aactgcggat 481 tctttctttc tcttccattc ttacgtttcc atattaaagt gtagtttttt tacttaaatt 541 taataatatt aatctaccaa tgtgagacca ttaattaaca acctgggcct catgggcctt 601 cctttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca 661 tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc 721 ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac 781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1561 gacagttatt agaaaaattc atccagcaga cgataaaacg caatacgctg gctatccggt 1621 gccgcaatgc catacagcac cagaaaacga tccgcccatt cgccgcccag ttcttccgca 1681 atatcacggg tggccagcgc aatatcctga taacgatccg ccacgcccag acggccgcaa 1741 tcaataaagc cgctaaaacg gccattttcc accataatgt tcggcaggca cgcatcacca 1801 tgggtcacca ccagatcttc gccatccggc atgctcgctt tcagacgcgc aaacagctct 1861 gccggtgcca ggccctgatg ttcttcatcc agatcatcct gatccaccag gcccgcttcc 1921 atacgggtac gcgcacgttc aatacgatgt ttcgcctgat gatcaaacgg acaggtcgcc 1981 gggtccaggg tatgcagacg acgcatggca tccgccataa tgctcacttt ttctgccggc 2041 gccagatggc tagacagcag atcctgaccc ggcacttcgc ccagcagcag ccaatcacgg 2101 cccgcttcgg tcaccacatc cagcaccgcc gcacacggaa caccggtggt ggccagccag 2161 ctcagacgcg ccgcttcatc ctgcagctcg ttcagcgcac cgctcagatc ggttttcaca 2221 aacagcaccg gacgaccctg cgcgctcaga cgaaacaccg ccgcatcaga gcagccaatg 2281 gtctgctgcg cccaatcata gccaaacaga cgttccaccc acgctgccgg gctacccgca 2341 tgcaggccat cctgttcaat catactcttc ctttttcaat attattgaag catttatcag 2401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 2461 gttccgcgca catttccccg aaaagtgcca c //