LOCUS Exported 7550 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein. ACCESSION . VERSION . KEYWORDS pCMV-OptoFGFR1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7550) AUTHORS Kim N, Kim JM, Lee M, Kim CY, Chang KY, Heo WD TITLE Spatiotemporal control of fibroblast growth factor receptor signals by blue light. JOURNAL Chem Biol. 2014 Jul 17;21(7):903-12. doi: 10.1016/j.chembiol.2014.05.013. Epub 2014 Jun 26. PUBMED 24981772 REFERENCE 2 (bases 1 to 7550) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7550 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 535..544 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3430..3439 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3436..4152 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3481..3502) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3742..3761) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4089..4110 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 4278..4399 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4315..4334) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4369..4388 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4406..4861) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4543..4564) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4755..4774 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4888..4992 /gene="bla" /label=AmpR promoter promoter 4994..5351 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5017..5037) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5202..5337 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5264..5283 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5386..6180 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5440..5459) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6050..6069 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6368..6387) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6412..6459 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6788..7376 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7277..7296 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 541 atgggatgta taaaatcaaa agggaaagac agcgcgggag cagatagtgc tggtagtgct 601 ggtagtgctg ggaattccaa gatgaagagt ggtaccaaga agagtgactt ccacagccag 661 atggctgtgc acaagctggc caagagcatc cctctgcgca gacaggtaac agtgtctgct 721 gactccagtg catccatgaa ctctggggtt cttctggttc ggccatcacg gctctcctcc 781 agtgggactc ccatgctagc aggggtctct gagtatgagc ttcccgaaga ccctcgctgg 841 gagctgcctc gggacagact ggtcttaggc aaacccctgg gagagggctg ctttgggcag 901 gtggtgttgg cagaggctat cgggctggac aaggacaaac ccaaccgtgt gaccaaagtg 961 gctgtgaaga tgttgaagtc ggacgcaaca gagaaagact tgtcagacct gatctcagaa 1021 atggagatga tgaagatgat cgggaagcat aagaatatca tcaacctgct gggggcctgc 1081 acgcaggatg gtcccttgta tgtcatcgtg gagtatgcct ccaagggcaa cctgcgggag 1141 tacctgcagg cccggaggcc cccagggctg gaatactgct acaaccccag ccacaaccca 1201 gaggagcagc tctcctccaa ggacctggtg tcctgcgcct accaggtggc ccgaggcatg 1261 gagtatctgg cctccaagaa gtgcatacac cgagacctgg cagccaggaa tgtcctggtg 1321 acagaggaca atgtgatgaa gatagcagac tttggcctcg cacgggacat tcaccacatc 1381 gactactata aaaagacaac caacggccga ctgcctgtga agtggatggc acccgaggca 1441 ttatttgacc ggatctacac ccaccagagt gatgtgtggt ctttcggggt gctcctgtgg 1501 gagatcttca ctctgggcgg ctccccatac cccggtgtgc ctgtggagga acttttcaag 1561 ctgctgaagg agggtcaccg catggacaag cccagtaact gcaccaacga gctgtacatg 1621 atgatgcggg actgctggca tgcagtgccc tcacagagac ccaccttcaa gcagctggtg 1681 gaagacctgg accgcatcgt ggccttgacc tccaaccagg agtacctgga cctgtccatg 1741 cccctggacc agtactcccc cagctttccc gacacccgga gctctacgtg ctcctcaggg 1801 gaggattccg tcttctctca tgagccgctg cccgaggagc cctgcctgcc ccgacaccca 1861 gcccagcttg ccaatggcgg actcaaacgc cgcgcggatc caccggtcgc caccatgaag 1921 atggacaaaa agaccatcgt ctggtttcgg agagatttga gaatagaaga taatcccgcg 1981 ctcgccgccg cggcccacga gggttccgtc ttccccgttt tcatttggtg tcctgaagaa 2041 gaaggccagt tttatcccgg aagggcctct aggtggtgga tgaagcaaag tctggcccat 2101 cttagccagt cactgaaagc actgggcagt gatcttaccc tgatcaagac acacaatacc 2161 atctctgcca tcctcgactg catcagggtg accggcgcaa cgaaagtcgt gtttaaccac 2221 ctgtacgatc cagttagtct ggtgcgcgac cacactgtga aggagaagct ggtggaacgg 2281 gggatcagtg tgcagagcta caacggggac cttctgtacg agccatggga gatctattgc 2341 gagaaaggga aaccgttcac ctccttcaac agttactgga agaaatgttt ggatatgtca 2401 atagagtccg ttatgttgcc ccctccctgg agactgatgc cgattactgc tgctgcagag 2461 gccatctggg cctgctccat cgaggaactc ggtctggaaa atgaagcaga aaagccaagc 2521 aatgcacttc tcactagagc ctggagcccc ggctggtcta atgccgacaa gctgcttaac 2581 gagttcatcg aaaaacaact gattgactac gcgaagaact ccaagaaagt ggtaggtaac 2641 tcaactagct tgctctctcc atatctccat tttggcgaga tttctgtccg ccatgtattt 2701 cagtgcgctc ggatgaaaca gattatctgg gctcgcgata aaaacagcga aggcgaagaa 2761 agcgccgatc tgttcctgcg agggatcgga cttcgggaat actcccggta tatatgtttc 2821 aactttccat tcacacacga gcagagtctg ttgtcccacc tcaggttctt cccctgggac 2881 gccgatgtcg acaaattcaa ggcatggaga cagggaagga cagggtaccc actcgtggat 2941 gctggcatga gagagctctg ggctacaggc tggatgcaca accgcatccg ggtaatcgtg 3001 tcctcatttg ctgtcaagtt tctgctcctg ccttggaaat ggggaatgaa gtacttttgg 3061 gatacccttc tcgacgccga cttggagtgt gacattctgg gatggcaata tattagcggg 3121 tcaattcctg acggccatga gttggacagg ttggacaatc cggccttgca gggagctaag 3181 tatgatcccg aaggagagta tattcgacag tggctccccg agctggcccg acttcctacg 3241 gagtggattc accatccttg ggacgcacca ctgacagtgc tcaaggcaag cggggtggag 3301 ctgggcacca attacgctaa gcctatagtt gatatagata cagcacgcga gctgctggct 3361 aaagcgatct ctcgcactcg ggaggcgcag attatgatcg gtgctgccgg cagcggcagc 3421 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 3481 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 3541 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 3601 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt tgcccgctac 3661 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 3721 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 3781 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 3841 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 3901 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 3961 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 4021 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 4081 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 4141 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag 4201 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 4261 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4381 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 4441 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 4501 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 4561 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 4621 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 4681 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 4741 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 4801 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 4861 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4921 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4981 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 5041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 5101 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 5161 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 5221 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 5281 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 5341 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 5401 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 5461 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 5521 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 5581 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 5641 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 5701 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 5761 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 5821 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 5881 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 5941 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 6001 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 6061 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 6121 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 6181 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 6241 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 6301 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 6361 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 6421 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 6481 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 6541 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 6601 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 6661 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 6961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 7021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 7081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 7141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 7201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 7261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 7321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 7381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 7441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa 7501 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg //