LOCUS Exported 2464 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-mPrrn::RBS (46%) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2464) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2464) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2464 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(670..687) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(780..1368) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(860..879) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1542..2336) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2337..2441) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggcctaggc gcgccatgag ctcggtctca ggaggctccc ccgccgtcgt tcaatgagaa 421 tggataagag gctcgtggga ttgacgtgag ggggcaggga tggcaatata tctgggagcg 481 aactccgggc gaatacgaag cgcttggata cagttgtagg gagggattta atgtgagacc 541 ggtacctctt aattaactgg cctcatgggc cttcctttca ctgcccgctt tccagtcggg 601 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct 661 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc 721 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 1081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 1141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 1261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 1381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 1441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 1501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa ttcatccagc 1561 agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag caccagaaaa 1621 cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag cgcaatatcc 1681 tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa acggccattt 1741 tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc ttcgccatcc 1801 ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg atgttcttca 1861 tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg ttcaatacga 1921 tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag acgacgcatg 1981 gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag cagatcctga 2041 cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac atccagcacc 2101 gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc atcctgcagc 2161 tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc ctgcgcgctc 2221 agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc atagccaaac 2281 agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc aatcatactc 2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 2461 ccac //