LOCUS Exported 2573 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-atpB 5'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2573) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2573) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2573 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(779..796) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(889..1477) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(969..988) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1651..2445) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2446..2550) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcatact caaatgaaag actttctcaa gattctgatt 421 catccacttg agattttaaa attaaaatag gttgggtggg cttgcaaatt cactcagtct 481 cagtgaataa gtaaacaatt gaatcggttc aattgcatgg tgccaacgaa atcgagtgct 541 aattcccatt ttattgaatt aaccgatcga cgtgctagcg gacatttatt ttgaattcga 601 taatttttgc aaaaacattt cgacatattt atttatttta ttattaatgt gagaccggta 661 ccctctgggc ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt 721 gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct 781 cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa 841 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 901 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 961 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 1021 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 1081 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 1141 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1201 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 1261 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 1321 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 1381 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1441 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1501 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1621 tatatatgag taaacttggt ctgacagtta ttagaaaaat tcatccagca gacgataaaa 1681 cgcaatacgc tggctatccg gtgccgcaat gccatacagc accagaaaac gatccgccca 1741 ttcgccgccc agttcttccg caatatcacg ggtggccagc gcaatatcct gataacgatc 1801 cgccacgccc agacggccgc aatcaataaa gccgctaaaa cggccatttt ccaccataat 1861 gttcggcagg cacgcatcac catgggtcac caccagatct tcgccatccg gcatgctcgc 1921 tttcagacgc gcaaacagct ctgccggtgc caggccctga tgttcttcat ccagatcatc 1981 ctgatccacc aggcccgctt ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg 2041 atgatcaaac ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg catccgccat 2101 aatgctcact ttttctgccg gcgccagatg gctagacagc agatcctgac ccggcacttc 2161 gcccagcagc agccaatcac ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg 2221 aacaccggtg gtggccagcc agctcagacg cgccgcttca tcctgcagct cgttcagcgc 2281 accgctcaga tcggttttca caaacagcac cggacgaccc tgcgcgctca gacgaaacac 2341 cgccgcatca gagcagccaa tggtctgctg cgcccaatca tagccaaaca gacgttccac 2401 ccacgctgcc gggctacccg catgcaggcc atcctgttca atcatactct tcctttttca 2461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac //