LOCUS Exported 2583 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-atpB 5'UTR +10 (functional) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2583) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2583) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2583 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(789..806) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(899..1487) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(979..998) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1661..2455) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2456..2560) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcatact caaatgaaag actttctcaa gattctgatt 421 catccacttg agattttaaa attaaaatag gttgggtggg cttgcaaatt cactcagtct 481 cagtgaataa gtaaacaatt gaatcggttc aattgcatgg tgccaacgaa atcgagtgct 541 aattcccatt ttattgaatt aaccgatcga cgtgctagcg gacatttatt ttgaattcga 601 taatttttgc aaaaacattt cgacatattt atttatttta ttattatgag aatcaaatgt 661 gagaccggta ccctctgggc ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga 721 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc 781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct 841 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1321 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 1381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 1621 caatctaaag tatatatgag taaacttggt ctgacagtta ttagaaaaat tcatccagca 1681 gacgataaaa cgcaatacgc tggctatccg gtgccgcaat gccatacagc accagaaaac 1741 gatccgccca ttcgccgccc agttcttccg caatatcacg ggtggccagc gcaatatcct 1801 gataacgatc cgccacgccc agacggccgc aatcaataaa gccgctaaaa cggccatttt 1861 ccaccataat gttcggcagg cacgcatcac catgggtcac caccagatct tcgccatccg 1921 gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc caggccctga tgttcttcat 1981 ccagatcatc ctgatccacc aggcccgctt ccatacgggt acgcgcacgt tcaatacgat 2041 gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg 2101 catccgccat aatgctcact ttttctgccg gcgccagatg gctagacagc agatcctgac 2161 ccggcacttc gcccagcagc agccaatcac ggcccgcttc ggtcaccaca tccagcaccg 2221 ccgcacacgg aacaccggtg gtggccagcc agctcagacg cgccgcttca tcctgcagct 2281 cgttcagcgc accgctcaga tcggttttca caaacagcac cggacgaccc tgcgcgctca 2341 gacgaaacac cgccgcatca gagcagccaa tggtctgctg cgcccaatca tagccaaaca 2401 gacgttccac ccacgctgcc gggctacccg catgcaggcc atcctgttca atcatactct 2461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 2521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 2581 cac //