LOCUS Exported 10263 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of APEX2 at nuclear pore. ACCESSION . VERSION . KEYWORDS V5-APEX2-SENP2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10263) AUTHORS Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY TITLE Atlas of Subcellular RNA Localization Revealed by APEX-Seq. JOURNAL Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 10.1016/j.cell.2019.05.027. PUBMED 31230715 REFERENCE 2 (bases 1 to 10263) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10263 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 65..368 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 369..572 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 523..543 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 595..619 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 640..681 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 682..1428 /codon_start=1 /product="soybean ascorbate peroxidase, engineered to serve as a monomeric tag for electron microscopy and live-cell proteomics (Lam et al., 2015)" /label=APEX2 /note="more active than the original APEX due to the A134P mutation" /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK YAADEDAFFADYAEAHQKLSELGFADA" misc_feature 3270..3858 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3323..3343) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3741..3752) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3930..4163 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4241..4362 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4278..4297) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4332..4351 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4402..4537 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4464..4483 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4557..4576) /label=T7 /note="T7 promoter, forward primer" promoter complement(4558..4576) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4586..4603) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4586..4602) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4595..4617) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4744..5199 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4831..4850) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5041..5062 /label=F1ori-F /note="F1 origin, forward primer" promoter 5225..5329 /gene="bla" /label=AmpR promoter CDS 5330..6190 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5548..5567) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6361..6949 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6850..6869 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7103..7120 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7237..7258 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7273..7303 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7311..7327 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7316..7338 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7335..7351 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7335..7351 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7370..7390 /label=T3 /note="T3 promoter, forward primer" promoter 7372..7390 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7418..7644 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7645..7825 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7872..7997 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8490..8723 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8908..8952 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9127..9627 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 9546..9564 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 9639..10034 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 10094..10211 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt 601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg gcaagcccat ccccaacccc 661 ctgctgggcc tggacagcac cggaaagtct tacccaactg tgagtgctga ttaccaggac 721 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct 781 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccggt 841 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt 901 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc 961 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca 1021 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcctgatccc 1081 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa 1141 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga 1201 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg 1261 ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct tttgtctgac 1321 cctgtattcc gccctctcgt tgataaatat gcagcggacg aagatgcctt ctttgctgat 1381 tacgctgagg ctcaccaaaa gctttccgag cttgggtttg ctgatgcctc cggccggact 1441 cagatctcga gctcaagctt cgaattcatg tacagatggc tggttaggat tctcggcacc 1501 attttccgtt tctgcgaccg gtcggtgccc cctgcccggg ccctcctgaa gaggcggcgc 1561 tcagacagca ctctgttttc tacagtggac actgatgaaa taccagccaa aagaccaaga 1621 ttagattgct ttattcacca agtgaaaaac agtctctaca atgctgccag cttatttgga 1681 ttcccattcc agctgaccac aaagcccatg gtaacttctg cttgtaatgg aacacggaat 1741 gtggcccctt caggagaggt attttcgaac tcttcatctt gtgaactgac aggttctgga 1801 tcctggaaca acatgctgaa actgggtaat aaatctccta atggaataag tgactatcca 1861 aagatcagag tgacagttac ccgagatcag ccacgcagag tcctgccttc ctttggtttt 1921 actttgaact cagaaggctg taatagaaga ccaggtggcc gtcgccatag caaaggtaat 1981 ccagagagtt ctttaatgtg gaaacctcag gaacaggctg taacagagat gatttctgaa 2041 gagagtggca agggtctgag gcgtccccat tgtactgtgg aggagggtgt tcaaaaagag 2101 gaaagagaga agtaccgaaa gttattggaa cgacttaaag aaagtggtca tggaaactct 2161 gtctgtcctg taacttcaaa ttatcacagt tctcaaagaa gtcagatgga cacattaaag 2221 accaaaggct ggggggaaga gcaaaatcac ggagtcaaaa caactcagtt tgttccaaaa 2281 caatatagac ttgttgaaac aaggggacct ctatgttcat tgagaagtga aaagaggtgt 2341 tcaaagggga aaattactga tacagagacg atggtcggaa tcagatttga aaatgaaagt 2401 aggaggggat accaactgga gcctgaccta tcagaagaag tgtcggcccg actccgcctg 2461 ggcagtggaa gcaatggctt actcaggagg aaagtgtcaa taattgagac aaaggaaaag 2521 aattgctcag gcaaagagag ggacagaaga acggacgatc tccttgaact tacagaggac 2581 atggaaaagg aaatcagtaa tgccctaggc catggcccac aggatgaaat cctaagtagt 2641 gctttcaaat tgcgaattac tcgaggagat attcagacat taaagaacta tcactggctc 2701 aatgatgaag tcattaattt ttacatgaat cttctggtgg aaagaaataa aaagcaaggc 2761 tatccagcac ttcatgtatt cagtactttc ttctatccta aattaaagtc tgggggttac 2821 caagcagtga aacgatggac caaaggggta aatctctttg aacaagaaat tattctggtg 2881 cctattcatc ggaaggtaca ttggagcctg gtggtgattg acctaagaaa aaagtgtctt 2941 aaatatctgg attctatggg acaaaagggc cacaggatct gtgagattct ccttcagtat 3001 ttacaggatg aaagtaagac caaaagaaat agtgatctga atcttttaga gtggacccat 3061 cacagcatga aaccacacga gattcctcaa cagctgaatg ggagtgattg tggaatgttt 3121 acttgtaaat atgcagatta tatttctagg gacaaaccta tcacatttac tcagcaccag 3181 atgcctctct tccggaagaa gatggtgtgg gaaatccttc atcagcagtt gctgtgagct 3241 agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa 3301 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 3361 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 3421 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 3481 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 3541 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 3601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 3661 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 3721 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 3781 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 3841 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct 3901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 3961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 4021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 4081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 4141 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta 4201 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 4261 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4321 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 4381 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4441 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4501 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct 4561 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 4621 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 4681 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 4741 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 4981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 5041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 5101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5161 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 5221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 5281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 5341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 5401 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 5461 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 5521 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 5581 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 5641 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 5701 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 5761 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 5821 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 5881 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 5941 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 6001 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 6061 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 6121 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 6181 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 6241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 6301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 6361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 6421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 6481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 6541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 6601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 6661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 6721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 6781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 6841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 6901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 6961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 7021 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 7081 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 7141 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 7201 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 7261 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 7321 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 7381 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg 7441 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac 7501 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag 7561 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt 7621 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag 7681 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 7741 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 7801 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga 7861 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg 7921 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 7981 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc 8041 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg 8101 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa 8161 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata 8221 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 8281 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg 8341 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa 8401 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg 8461 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca 8521 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg 8581 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg 8641 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga 8701 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc 8761 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac 8821 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta 8881 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa 8941 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc 9001 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg 9061 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg 9121 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 9181 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 9241 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 9301 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 9361 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 9421 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 9481 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 9541 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 9601 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag 9661 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag 9721 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg 9781 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg 9841 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga 9901 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag 9961 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt 10021 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag 10081 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 10141 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa 10201 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg 10261 ggg //