LOCUS Exported 2376 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-rbcL 5'UTR (processed) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2376) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2376) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2376 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(582..599) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(692..1280) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(772..791) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1454..2248) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2249..2353) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcatact gtcgagtaga ccttgttgtt gtgagaattc 421 ttaattcatg agttgtaggg agggatttaa tgtgagaccg gtaccctctg ggcctcatgg 481 gccttccttt cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat 541 ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg 601 cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca 661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 1021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 1081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 1141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1441 ggtctgacag ttattagaaa aattcatcca gcagacgata aaacgcaata cgctggctat 1501 ccggtgccgc aatgccatac agcaccagaa aacgatccgc ccattcgccg cccagttctt 1561 ccgcaatatc acgggtggcc agcgcaatat cctgataacg atccgccacg cccagacggc 1621 cgcaatcaat aaagccgcta aaacggccat tttccaccat aatgttcggc aggcacgcat 1681 caccatgggt caccaccaga tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca 1741 gctctgccgg tgccaggccc tgatgttctt catccagatc atcctgatcc accaggcccg 1801 cttccatacg ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca aacggacagg 1861 tcgccgggtc cagggtatgc agacgacgca tggcatccgc cataatgctc actttttctg 1921 ccggcgccag atggctagac agcagatcct gacccggcac ttcgcccagc agcagccaat 1981 cacggcccgc ttcggtcacc acatccagca ccgccgcaca cggaacaccg gtggtggcca 2041 gccagctcag acgcgccgct tcatcctgca gctcgttcag cgcaccgctc agatcggttt 2101 tcacaaacag caccggacga ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc 2161 caatggtctg ctgcgcccaa tcatagccaa acagacgttc cacccacgct gccgggctac 2221 ccgcatgcag gccatcctgt tcaatcatac tcttcctttt tcaatattat tgaagcattt 2281 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 2341 taggggttcc gcgcacattt ccccgaaaag tgccac //