LOCUS       Exported               13274 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene 
            (NM_001109626.1 isoform) cloned into a dox-inducible piggybac 
            destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    PB-Neo-TetO-Cdk12_Nterm_FlagHa
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Dubbury SJ, Boutz PL, Sharp PA
  TITLE     CDK12 regulates DNA repair genes by suppressing intronic 
            polyadenylation.
  JOURNAL   Nature. 2018 Dec;564(7734):141-145. doi: 10.1038/s41586-018-0758-y. 
            Epub 2018 Nov 28.
  PUBMED    30487607
REFERENCE   2  (bases 1 to 13274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1021..1245
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1305..1346)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1348..1372
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5844..5870)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5871..5894)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5894..5903
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(5933..5957)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6069..6093)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6090..6128)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    6161..6431
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    6534..6645
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(6683..7486)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6794..6813)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7404..7423
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(7548..7567)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7941..7964
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(7990..8199)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7995..8130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8000..8297)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8049..8068)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8338..8354
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8339..8355
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8704..8738
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9231..9247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9231..9247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9244..9266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9255..9271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9279..9309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9324..9345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9462..9479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9586..10089
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10130..10148
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10186..10208)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10308..10327
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10351..10566
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10567..11370
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(11527..12115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11607..11626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12286..13146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12909..12928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13147..13251)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc ctgcagccca gcttgatccc gcctcagaag
     1021 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     1081 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     1141 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     1201 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     1261 gggtttaaac gggccctcta gaggttagcg ctagctcatt actacgtaga atcgagaccg
     1321 aggagagggt tagggatagg cttaccaacc actttgtaca agaaagctgg gtcgaattcg
     1381 cccttctcga gttaataagg aactcctctc cctcttcccc ctcgtggtag gactcttgca
     1441 gcccccctgt agggttttcc ataagactga gctgggcccc cccactgaag cgttgttcct
     1501 gagctgttgg ccccagtggt tcccggtccc agctccccat agttttgcaa tttggtctct
     1561 gcatgtacca cagagttgct atttccctca gacttgagga atggttgccc aaccactgag
     1621 tgtaatgcta agggaaccct ggtaaaacgg aggtcctggt cccctgggaa ctgcactgac
     1681 cctgtgccct ggtagctgtt ggactcccca aggtggctca cagagcctga gaaggtcctg
     1741 ttcttcaccg gggtctgaac caaagattct gtcaaggctg gaccagaact gcgttcatca
     1801 gtgtcagcgg aactgaaccc tgtctcaggc ccatcagtat ttccgtaagt cctgtttgga
     1861 tgggatcggg tggagtattc cattggtctc aaggcctggt gctcatgtgg caggtggcca
     1921 ggaggctctg cttcaggctg ggataaaagt tgcagcaagg cggctgtcac ggcgggattc
     1981 aactcctggg gggcgctgga aagatcgcct tcaaccagag ggggtggagg tggcggcggt
     2041 ggaggtccag ggggctcagg gggcctcttc tctggtggaa gaatgtgagg aggacatgct
     2101 gctgcctcct cctgtggcat tgtaggagtt cttcggggcc cctgtggccc actattcttg
     2161 tcattggtgt tttcctccag gttacctgct ggctcttggg ttttcatcag ctgactcaag
     2221 agaactgcca acacattctg catgtcagct ggtgtgtttg aagcttcagg agttgtctgt
     2281 tcagcaggag gcaggaccac aggtacagaa ggtacctcct tcaatgattc ttcaggggct
     2341 atggattctg agtcctgctg ctgggaactg gcttcagtca gtgcactaat agactgattc
     2401 aaggcttcaa gctgctgttg catctctgga ttggagtgga tattaagcag ctgtgccatc
     2461 tgagggatgc tcaggtcagt ttggctctga agcaggttta ataacactgc caattcactt
     2521 tgattcaact gctgtgtgat gtcaccaagg cctactgcat cccctggacc aggctctgcc
     2581 ttgacaggag caggctgagg tggtgctggg ctattgtttt tcacaggctc agctgttgtc
     2641 cctgaggtag tttcttttct agaagctttg gacggaggtg gatcttctat cacaatacca
     2701 ctctgtcgct gccgtcgacg tttcttactc cacaattcat ggcaatcctg ccagtgaggt
     2761 aggtctggag gtgccatttt gctgagttcc acatctttaa gaaagtcact ctgtagggtc
     2821 tgttcagctg tgcacctctt gctaggatcc agtgtcagca tgtggtccaa tagatcaagt
     2881 gccgctgaag gaatgaaaga gaattcttct cttaggcgtc tcctgtattg cttcttcggt
     2941 ttcatggtgt tgaagtaggg cagcttgata acatcaggcc acactgctgg acaaggacta
     3001 ccacagagac gactgataag ttctagctga gccagttcta aattggcttg aaaaataggt
     3061 ttctttgtga acagttctcc aaggatgcac ccacagctcc aaacatcaat ggctggtgtg
     3121 tatctttcct ctccaagaag aagctctgga ggtcgatacc aaagagtgat gactttgttt
     3181 gtgtaagggc gactctcttc agagttatag agccgagcaa gtccaaaatc tgccagtttg
     3241 atttgcccgc tgttattcag caaaatgtta gaacatttaa tatcccgatg gaggaaattc
     3301 tttttgtgac agtaatccag tccttccatt agctgtttca taaatgactt gatatggtcc
     3361 tcagaaaagt gcaccaaacc tgattcaagc agtcccatta agtcatggtc catatattca
     3421 aatacaaggt aaaaggcacc tttgtccttc ttgaaatcca gtgcatcttg tttgtctgtg
     3481 acaatttcct tcatgtttac aacactctgg tgaactaatt gacgaagaat cttgatctcc
     3541 cggatggctg tgattgggaa gccctctttc tcattgtcca gccgaacctt cttcagagct
     3601 actagttctc ctgtgtcttt gtccttggct ttatatactt ggccataggt cccctctcca
     3661 ataatcccaa taatgtcaaa cttgtccaca cagcgcttcc cccaatcact ttctgtttgt
     3721 cttctttctc cataacgtgg acaacaaatt tttggtctct ttttatatgg ttgttgaggt
     3781 ggtgtaattg cctttggctc tggagaatct ggaggcgatg gatctcctcc tggtagctca
     3841 ggaggaagag gcaagtctgt aagcaagtgg cgagtcctct gttccttctc tttctttgca
     3901 ggctttgaag gaagtgtttc ttttggacta tccatgtcat catctccagg taatagggga
     3961 gggaggggca gaggaggcaa tgttgaagtc ttcagatgtg gaatagcagc tgttatagat
     4021 acttgagtct tcatagaaac ctgtacaggg ggctgagaat ttgtctgaga ggatagagta
     4081 gatgtccttg ggtgaggaga agagggtaaa attgaagtac ttgaaacagg aacttgacta
     4141 aatggtggtt ggggaggagg cagaggtggc tgcagcggaa tagctggtag tggaggcaaa
     4201 ggtggcaaag ggggtgtctg aggtggaggg gtagtagctg gtaaaggggg tggagaagta
     4261 attgtgggaa gaggtggaag ggtctccttt tctgatgtct ctgtctcctt tgaagtaaca
     4321 atcacctctt tcggtgctac aggtttaacg tcctttgttc cctgtgcttt caaatcctta
     4381 gcaggatgct tctgcaagtt ctcatccaac ctaaccttcc ctgtgtctaa acaatgtttt
     4441 acctctgggt ttgaatgtgc tacagtaacc agttcagtat ctggggtcga tttttctgat
     4501 tttatacttc tgggtaactt tttagactct aaccctgact ccttcacctc aagtttctct
     4561 tttttaggca aaattatagg tgaactcttg gactcttttc catccatttt tgctgctgca
     4621 gcagctgctg ctctttcctt cttttttcta ctgagttcag ctcccaggct ggaattcaat
     4681 ggaagcctga caggtgagat actggaatga cgactgcgtg acccggatcg cttcttttta
     4741 ctatgagaag atgagtgtct tgaatatgca ggacttctac ttctggactt catggatttc
     4801 ctactgggga gtggactccg actcagagac ctcttgctca ggaaagggct gcttgatcgc
     4861 cttcggccat aggggctggg tgatctcccg ctgtaagagc cgctcctttc atagctggag
     4921 gaccgtctcc ggctataggg actcacagac ctctgtcgtc ggctgtaagg actgggtgac
     4981 cgagtgctgg actggtaagc agaaggctct ttgtaaggtg ggctgattga ctgccttctt
     5041 gaggtacttc caggactctt cttgtaggag tcatagttgc tagaagtgtg agaccttggg
     5101 gggctaagat cgtagtcttg gccataagaa gctccggaag ggctgtcatc ttgcttggaa
     5161 ctgtcagacc atttcctatg gggactcctg gatctccgtt tagggctagc cacggttttg
     5221 taacttttgg gtgtttccct tttccgatga cttttactcc ggtccttgta tccagacttt
     5281 aactctcgct ctttgcgggt cttctccttg tgcattttgg acgacctgga ttccttgctg
     5341 ccgcttttgg atagctgggc cttcccataa tcatcactcc cctccacgga ccgtttggaa
     5401 ctgcccgata cccggtcctt catagatcca gatttggaga cttcctgatt tttttccttt
     5461 tccgtctgtt tagtttttag caagtctcgg gaccgccggt gctggtggtg acgatgtcgg
     5521 tgcaggcgat cgctccgatc cgttccccga cgttcctcgt tctccctccg gtctgatttg
     5581 aaggccgtgt catcggagaa ggtgtctgaa tcagagctga tgtcatcgta ctccaccagt
     5641 ggtttgatta tggtacccaa agatgccgct tcgggggtca ccagccccac gtctttggag
     5701 tgcttggact tatgccgctt gtgcttcgac accaagcggt gacgctccct gctgttggag
     5761 ctgccacctc ccgatgacgg ctgcgaagtt ccagaagctc ctccgctccc gtccttcttg
     5821 cccccatgtc tctcggaatt gggagcgtaa tcgggcacgt cataagggta cttgtcatcg
     5881 tcgtccttgt agtccatggt ggcggcgagc tcagtagtaa gggcgaattc ggagcctgct
     5941 tttttgtaca aacttgttga tatctgcact agtggatccg agctcggtac gaattcgggg
     6001 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
     6061 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
     6121 cgcctacctc gacccgggta ccgagctcga ctttcacttt tctctatcac tgatagggag
     6181 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     6241 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     6301 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     6361 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     6421 actgataggg agtggtaaac tcgagttgcc tgctattgtc ttcccaatcc tcccccttgc
     6481 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     6541 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     6601 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     6661 gagctctaga gaattgatcc cctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     6721 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     6781 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     6841 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     6901 agcaggcatc gccatgggtc acgacgagat catcgccgtc gggcatgcgc gccttgagcc
     6961 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     7021 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     7081 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     7141 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     7201 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     7261 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     7321 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     7381 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     7441 ccggagaacc tgcgtgcaat ccatcttgtt caatggccga tcccatattg gctgcaggtc
     7501 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
     7561 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
     7621 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
     7681 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
     7741 tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
     7801 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
     7861 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
     7921 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
     7981 aaaagtactg cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     8041 agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     8101 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     8161 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     8221 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     8281 tggggacttt ccacacccta actgacacac acagcagaga tccagtttgg ttagatctcg
     8341 aggtcgacgg tatcgttggc cggtactcga cgtagggata agatcggaag agcggttcag
     8401 caggaatgcc gagaccgcgc caacgataag ctatctataa caagaaaata tatatataat
     8461 aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta
     8521 aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa
     8581 tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga
     8641 tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga
     8701 atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg catcaggatc
     8761 atatcgtcgg gtcttttttc aattgctcag tcatcgccca agctggcgct atctgggcat
     8821 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     8881 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     8941 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     9001 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     9061 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     9121 tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg
     9181 gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg
     9241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9541 tccacagaat caggggataa cgcaggaaag acctgggtcc ttttcatcac gtgctataaa
     9601 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa
     9661 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg
     9721 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt
     9781 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc
     9841 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag
     9901 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
     9961 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
    10021 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
    10081 caagcgatcc gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10141 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10201 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10261 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10321 gattgtactg agagtgcacc ataccacctt ttcaattcat catttttttt ttattctttt
    10381 ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa
    10441 cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca
    10501 tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga
    10561 taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct
    10621 gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt
    10681 cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta
    10741 aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag
    10801 gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt
    10861 aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt
    10921 acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa
    10981 gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc
    11041 ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt
    11101 gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg
    11161 attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga
    11221 accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt
    11281 gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca
    11341 tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata
    11401 ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga
    11461 aataccgcac agagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11641 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11701 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11761 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11821 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11881 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11941 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    12001 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12061 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12121 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12181 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12241 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12301 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12361 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12421 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12481 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12541 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12601 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12661 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12721 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12781 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12841 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12901 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12961 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13021 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13081 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13141 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13201 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13261 ccgaaaagtg ccac
//
