LOCUS Exported 2381 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-T7G10 leader SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2381) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2381) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2381 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" RBS 424..446 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" primer_bind complement(587..604) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(697..1285) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(777..796) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1459..2253) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2254..2358) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcatact ggcagaccac aacggtttcc cactagaaat 421 aattttgttt aactttaaga aggagatata cataatgtga gaccggtacc ctctgggcct 481 catgggcctt cctttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 541 aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact 601 cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa 661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 1021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 1141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 1201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1441 aacttggtct gacagttatt agaaaaattc atccagcaga cgataaaacg caatacgctg 1501 gctatccggt gccgcaatgc catacagcac cagaaaacga tccgcccatt cgccgcccag 1561 ttcttccgca atatcacggg tggccagcgc aatatcctga taacgatccg ccacgcccag 1621 acggccgcaa tcaataaagc cgctaaaacg gccattttcc accataatgt tcggcaggca 1681 cgcatcacca tgggtcacca ccagatcttc gccatccggc atgctcgctt tcagacgcgc 1741 aaacagctct gccggtgcca ggccctgatg ttcttcatcc agatcatcct gatccaccag 1801 gcccgcttcc atacgggtac gcgcacgttc aatacgatgt ttcgcctgat gatcaaacgg 1861 acaggtcgcc gggtccaggg tatgcagacg acgcatggca tccgccataa tgctcacttt 1921 ttctgccggc gccagatggc tagacagcag atcctgaccc ggcacttcgc ccagcagcag 1981 ccaatcacgg cccgcttcgg tcaccacatc cagcaccgcc gcacacggaa caccggtggt 2041 ggccagccag ctcagacgcg ccgcttcatc ctgcagctcg ttcagcgcac cgctcagatc 2101 ggttttcaca aacagcaccg gacgaccctg cgcgctcaga cgaaacaccg ccgcatcaga 2161 gcagccaatg gtctgctgcg cccaatcata gccaaacaga cgttccaccc acgctgccgg 2221 gctacccgca tgcaggccat cctgttcaat catactcttc ctttttcaat attattgaag 2281 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2341 acaaataggg gttccgcgca catttccccg aaaagtgcca c //