LOCUS       Exported                3734 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    pMK-Lux-C codon opt. Nt chloroplasts
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1940..1957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2050..2638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2130..2149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2812..3606)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(3607..3711)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg gtctcaaatg attaaaaaaa ttcctatgat tattggagga gtagtacaaa
      421 atacttctgg atatggaatg agagaattaa ctttaaataa taataaagta aatattccta
      481 ttattactca atctgatgta gaagctattc aatctttaaa tattgaaaat aaattaacta
      541 ttaatcaaat tgtaaatttt ttatatactg taggacaaaa atggaaatct gaaacttatt
      601 ctagaagatt aacttatatt agagatttaa ttaaattttt aggatattct caagaaatgg
      661 ctaaattaga agctaattgg atttctatga ttttatgttc taaatctgct ttatatgata
      721 ttgtagaaaa tgatttatct tctagacata ttattgatga atggattcct caaggagaat
      781 gttatgtaaa agctttacct aaaggaaaat ctgtacattt attagctgga aatgtacctt
      841 tatctggagt aacttctatt ttaagagcta ttttaactaa aaatgaatgt attattaaaa
      901 cttcttctgc tgatcctttt actgctactg ctttagtaaa ttcttttatt gatgtagatg
      961 ctgaacatcc tattactaga tctatttctg taatgtattg gtcccattct gaagatttag
     1021 ctattcctaa acaaattatg tcttgtgctg atgtagtaat tgcttgggga ggagatgatg
     1081 ctattaaatg ggctactgaa catgctcctt ctcatgctga tattttaaaa tttggaccta
     1141 aaaaatctat ttctattgta gataatccta ctgatattaa agctgctgct attggagtag
     1201 ctcatgatat ttgtttttat gatcaacaag cttgtttttc tactcaagat atttattata
     1261 ttggagattc tattgatatt ttttttgatg aattagctca acaattaaat aaatataaag
     1321 atattttacc taaaggagaa agaaattttg atgaaaaagc tgctttttct ttaactgaaa
     1381 gagaatgttt atttgctaaa tataaagtac aaaaaggaga atctcaatct tggttattaa
     1441 ctcaatctcc tgctggatct tttggaaatc aacctttatc tagatctgct tatattcatc
     1501 aagtaaatga tatttctgaa gtaattcctt ttgtacataa agctgtaact caaactgtag
     1561 ctattgctcc ttgggaatct tcttttaaat atagagatat tttagctgaa catggagctg
     1621 aaagaattat tgaagctgga atgaataata tttttagagt aggaggagct catgatggaa
     1681 tgaggccttt acaaagatta gtaaattata tttctcatga aagaccttct acttatacta
     1741 ctaaagatgt atctgtaaaa attgaacaaa ctagatattt agaagaagat aaatttttag
     1801 tatttgtacc ttaggctttg agaccctggg cctcatgggc cttcctttca ctgcccgctt
     1861 tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt
     1921 attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc
     1981 ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa
     2821 ttcatccagc agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag
     2881 caccagaaaa cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag
     2941 cgcaatatcc tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa
     3001 acggccattt tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc
     3061 ttcgccatcc ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg
     3121 atgttcttca tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg
     3181 ttcaatacga tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag
     3241 acgacgcatg gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag
     3301 cagatcctga cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac
     3361 atccagcacc gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc
     3421 atcctgcagc tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc
     3481 ctgcgcgctc agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc
     3541 atagccaaac agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc
     3601 aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3721 ccgaaaagtg ccac
//
