LOCUS Exported 2474 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-rbcL 3'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2474) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2474) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2474 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(680..697) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(790..1378) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(870..889) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1552..2346) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2347..2451) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccacgtg tcttgtccag agctcggtct cagcttaaac agtagacatt agcagataaa 421 ttagcaggaa ataaagaagg ataaggagaa agaactcaag taattatcct tcgttctctt 481 aattgaattg caattaaact cggcccaatc ttttactaaa aggattgagc cgaataccgc 541 ttgagaccgg tacctggagc acaagactgg cctcatgggc cttcctttca ctgcccgctt 601 tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt 661 attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc 721 ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 1141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 1201 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 1261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 1321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 1381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 1441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 1501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa 1561 ttcatccagc agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag 1621 caccagaaaa cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag 1681 cgcaatatcc tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa 1741 acggccattt tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc 1801 ttcgccatcc ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg 1861 atgttcttca tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg 1921 ttcaatacga tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag 1981 acgacgcatg gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag 2041 cagatcctga cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac 2101 atccagcacc gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc 2161 atcctgcagc tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc 2221 ctgcgcgctc agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc 2281 atagccaaac agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc 2341 aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 2401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 2461 ccgaaaagtg ccac //