LOCUS Exported 2505 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-petD 3'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2505) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2505) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2505 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(711..728) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(821..1409) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(901..920) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1583..2377) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2378..2482) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatc aacgagctcg gtctcagctt atttttaaat tgattcaatt gtgaaataac 421 acgacatgtg tatctaggga atagtttctt caaagcgaat tctccctaga tacatctatt 481 caatttaatt ctgaatttat tttgaatata tgatatatta atatattaat tgtgctaaag 541 agtttcaatc tattttcact aagtaagtcc aatagatcgc ttgagaccgg taccctctgg 601 gcctcatggg ccttcctttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 661 cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact 721 gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc 781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 1021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 1081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 1141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 1201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 1261 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 1321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 1381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 1441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 1501 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 1561 agtaaacttg gtctgacagt tattagaaaa attcatccag cagacgataa aacgcaatac 1621 gctggctatc cggtgccgca atgccataca gcaccagaaa acgatccgcc cattcgccgc 1681 ccagttcttc cgcaatatca cgggtggcca gcgcaatatc ctgataacga tccgccacgc 1741 ccagacggcc gcaatcaata aagccgctaa aacggccatt ttccaccata atgttcggca 1801 ggcacgcatc accatgggtc accaccagat cttcgccatc cggcatgctc gctttcagac 1861 gcgcaaacag ctctgccggt gccaggccct gatgttcttc atccagatca tcctgatcca 1921 ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg atgtttcgcc tgatgatcaa 1981 acggacaggt cgccgggtcc agggtatgca gacgacgcat ggcatccgcc ataatgctca 2041 ctttttctgc cggcgccaga tggctagaca gcagatcctg acccggcact tcgcccagca 2101 gcagccaatc acggcccgct tcggtcacca catccagcac cgccgcacac ggaacaccgg 2161 tggtggccag ccagctcaga cgcgccgctt catcctgcag ctcgttcagc gcaccgctca 2221 gatcggtttt cacaaacagc accggacgac cctgcgcgct cagacgaaac accgccgcat 2281 cagagcagcc aatggtctgc tgcgcccaat catagccaaa cagacgttcc acccacgctg 2341 ccgggctacc cgcatgcagg ccatcctgtt caatcatact cttccttttt caatattatt 2401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 2461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac //