LOCUS Exported 2438 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoChlo kit. ACCESSION . VERSION . KEYWORDS pMK-TYMV 3'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2438) AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S TITLE MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PUBMED 30679266 REFERENCE 2 (bases 1 to 2438) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2438 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(644..661) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(754..1342) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(834..853) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1516..2310) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2311..2415) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccacgtg tcttgtccag agctcggtct cagcttgttc tcgatcttta aaatcgttag 421 ctcgccagtt agcgaggtct gtccccacac gacagataat cgggtgcaac tcccgcccct 481 tttccgaggg tcatcggaac ccgcttgaga ccggtacctg gagcacaaga ctggcctcat 541 gggccttcct ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac 601 atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc 661 tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc 721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1501 ttggtctgac agttattaga aaaattcatc cagcagacga taaaacgcaa tacgctggct 1561 atccggtgcc gcaatgccat acagcaccag aaaacgatcc gcccattcgc cgcccagttc 1621 ttccgcaata tcacgggtgg ccagcgcaat atcctgataa cgatccgcca cgcccagacg 1681 gccgcaatca ataaagccgc taaaacggcc attttccacc ataatgttcg gcaggcacgc 1741 atcaccatgg gtcaccacca gatcttcgcc atccggcatg ctcgctttca gacgcgcaaa 1801 cagctctgcc ggtgccaggc cctgatgttc ttcatccaga tcatcctgat ccaccaggcc 1861 cgcttccata cgggtacgcg cacgttcaat acgatgtttc gcctgatgat caaacggaca 1921 ggtcgccggg tccagggtat gcagacgacg catggcatcc gccataatgc tcactttttc 1981 tgccggcgcc agatggctag acagcagatc ctgacccggc acttcgccca gcagcagcca 2041 atcacggccc gcttcggtca ccacatccag caccgccgca cacggaacac cggtggtggc 2101 cagccagctc agacgcgccg cttcatcctg cagctcgttc agcgcaccgc tcagatcggt 2161 tttcacaaac agcaccggac gaccctgcgc gctcagacga aacaccgccg catcagagca 2221 gccaatggtc tgctgcgccc aatcatagcc aaacagacgt tccacccacg ctgccgggct 2281 acccgcatgc aggccatcct gttcaatcat actcttcctt tttcaatatt attgaagcat 2341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2401 aataggggtt ccgcgcacat ttccccgaaa agtgccac //