LOCUS       Exported                5059 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Localization: Plasma Membrane, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    mIFP12-Farnesyl-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      348..936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     837..856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1111..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1415..1618
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1569..1589
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1663..2622
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    2897..3018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2934..2953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2988..3007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3025..3480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3162..3183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3374..3393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3507..3611
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3613..3970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3636..3656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3821..3956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3883..3902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4005..4799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4059..4078)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4669..4688
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4987..5006)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5031..19
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
       61 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
      121 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
      181 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1021 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1441 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1501 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1561 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     1621 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatgtcggt accgctgact
     1681 acctcagcat tcggccacgc gtttctggct aactgtgaac gcgagcagat ccacctggcg
     1741 ggctccattc agccgcacgg tatcctgctg gctgtgaaag agccggacaa cgtggtgatc
     1801 caggcttcta ttaacgctgc ggagttcctg aacaccaact ctgttgttgg ccgtccgctg
     1861 cgtgacctgg gcggcgatct gcctttgcag atcctgccgc acctgaacgg cccgctgcac
     1921 ctggctccga tgaccctgcg ttgtaccgtg ggttctccgc cgcgtcgtgt ggactgtacc
     1981 attcatcgtc cgtctaacgg cggcctgatc gtagaactgg aaccagcaac caagaccact
     2041 aacattgcgc cggctctgga cggtgcgttt catcgtatca cttcttcatc ctccctgatg
     2101 ggcctgtgtg acgaaaccgc gactattatc cgtgagatta ctggctacga ccgtgtgatg
     2161 gtagtacgtt tcgatgaaga gggtaatggc gaaattctgt ccgaacgtcg tcgtgcggac
     2221 ctggaagcgt tcctgggtaa ccgctacccg gcgtctacta ttccgcagat cgctcgtcgc
     2281 ctgtacgaac ataaccgtgt tcgcctgctg gtagatgtga actatactcc ggttccgcta
     2341 cagccgcgca tcagcccgct gaacggtcgt gatctggata tgtccctgtc ttgcctgcgc
     2401 tctatgtccc cgatccacca gaaatacatg caggacatgg gcgttggcgc gaccctggtt
     2461 tgctctctga tggtgtctgg tcgtctgtgg ggtctgatcg cttgccacca ctacgaaccg
     2521 cgcttcgttc cgttccacat tcgcgctgct ggcgaagcgc tggcggaaac ttgtgcgatc
     2581 cgcatcgcga cgctggagag ctttgcacag tctcagtcca aaggtggcgg aagcggcggc
     2641 ggaagcggtg gcggaagttc cggactcaga tctaagctga accctcctga tgagagtggc
     2701 cccggctgca tgagctgcaa gtgtgtgctc tcctgagaat tctgcagtcg acggtaccgc
     2761 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     2821 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2881 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3001 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     3061 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3121 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3181 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3241 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3301 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3361 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3421 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3481 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3541 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3601 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3721 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3781 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3901 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3961 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4021 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4081 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4141 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     4201 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4261 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4321 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4381 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4441 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4501 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     4561 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4621 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4681 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4741 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4801 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4861 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4921 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     4981 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     5041 aagacagaat aaaacgcac
//
