LOCUS Exported 11842 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION expression of CHR2 mCherry from EF1a promoter. ACCESSION . VERSION . KEYWORDS EF1a CHR2 mCherry WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11842) TITLE Emory Custom Cloning Core Plasmids - Oskar Laur REFERENCE 2 (bases 1 to 11842) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11842 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(56..75) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 247..626 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 628..826 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 781..801 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 844..1024 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1071..1196 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1689..1922 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2107..2151 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2449..2566 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2704..3882 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2935..3873 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3830..3850 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 3899..3918 /label=T7 /note="T7 promoter, forward primer" promoter 3899..3917 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3974..3983 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3980..4906 /codon_start=1 /product="H134R mutant of an N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2005)" /label=ChR2(H134R) /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGAV" regulatory 4916..4925 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4922..5632 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5067..5085) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 5335..5354 /label=mCherry-F /note="mCherry, forward primer" misc_feature 5643..6231 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5696..5716) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6114..6125) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" misc_feature 6287..6873 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6454..6471) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6681..6700 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6874..7473 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6874..6893) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7370..7390 /label=Puro-F /note="Puromycin resistance gene, forward primer" protein_bind 7589..7622 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 7647..7827 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7853..7870) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7859..8083 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8129..8557 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8216..8235) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8426..8447 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8566..8586) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8571..8900 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8751..8886 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8813..8832 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8948..8995 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9014..9388 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9518..9639 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9555..9574) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9609..9628 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9688..9704) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9688..9704) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9701..9723) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9712..9728 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9736..9766) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9781..9802 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9919..9936) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10090..10678) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10170..10189) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10849..11709) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11472..11491 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11710..11814) /gene="bla" /label=AmpR promoter ORIGIN 1 atthrstncg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 1561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 1621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 1681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 1741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 1801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1921 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 2041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 2101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 2161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 2221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 2281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 2341 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg 2401 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa 2461 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2521 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 2581 ggacagcaga gatccagttt ggttaattaa tcgataagct ttgcaaagat ggataaagtt 2641 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctcg 2701 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 2761 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa 2821 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 2881 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt 2941 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga 3001 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 3061 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 3121 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct 3181 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 3241 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 3301 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 3361 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 3421 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 3481 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 3541 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 3601 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 3661 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 3721 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 3781 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 3841 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag cttggtacta 3901 atacgactca ctatagggag acccaagctg gctaggtaag cttgatatcg aattcctgca 3961 gcccggggga tccgccacca tggactatgg cggcgctttg tctgccgtcg gacgcgaact 4021 tttgttcgtt actaatcctg tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg 4081 ttactgtgcc ggatggattg aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt 4141 cctgcagtgg cttgcagcag gattcagcat tttgctgctg atgttctatg cctaccaaac 4201 ctggaaatct acatgcggct gggaggagat ctatgtgtgc gccattgaaa tggttaaggt 4261 gattctcgag ttcttttttg agtttaagaa tccctctatg ctctaccttg ccacaggaca 4321 ccgggtgcag tggctgcgct atgcagagtg gctgctcact tgtcctgtca tccttatccg 4381 cctgagcaac ctcaccggcc tgagcaacga ctacagcagg agaaccatgg gactccttgt 4441 ctcagacatc gggactatcg tgtggggggc taccagcgcc atggcaaccg gctatgttaa 4501 agtcatcttc ttttgtcttg gattgtgcta tggcgcgaac acattttttc acgccgccaa 4561 agcatatatc gagggttatc atactgtgcc aaagggtcgg tgccgccagg tcgtgaccgg 4621 catggcatgg ctgtttttcg tgagctgggg tatgttccca attctcttca ttttggggcc 4681 cgaaggtttt ggcgtcctga gcgtctatgg ctccaccgta ggtcacacga ttattgatct 4741 gatgagtaaa aattgttggg ggttgttggg acactacctg cgcgtcctga tccacgagca 4801 catattgatt cacggagata tccgcaaaac caccaaactg aacatcggcg gaacggagat 4861 cgaggtcgag actctcgtcg aagacgaagc cgaggccgga gccgtgccag gatccgccac 4921 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4981 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 5041 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 5101 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 5161 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 5221 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 5281 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 5341 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 5401 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 5461 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5521 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5581 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aggcttatcg 5641 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 5701 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5761 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5821 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5881 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5941 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6001 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 6061 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6121 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6181 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 6241 gtgtacaagc ggccgcgtcg acgggcccgc ggaattccgc cccccccccc tctccctccc 6301 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 6361 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 6421 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 6481 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 6541 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 6601 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 6661 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 6721 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 6781 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 6841 tttgaaaaac acgatgataa tatggccaca accatgaccg agtacaagcc cacggtgcgc 6901 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 6961 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 7021 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 7081 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 7141 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 7201 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 7261 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 7321 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 7381 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 7441 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg 7501 accgaaagga gcgcacgacc ccatgcatcg aacaaaagaa aaggggggac tggaagggct 7561 aattcactcc caacgaagac aagatatcat aacttcgtat agcatacatt atacgaagtt 7621 atcggctagc tggtccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 7681 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 7741 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 7801 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact 7861 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 7921 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 7981 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 8041 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 8101 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 8161 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 8221 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 8281 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 8341 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 8401 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 8461 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 8521 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 8581 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 8641 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 8701 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 8761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 8821 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 8881 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 8941 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt 9001 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 9061 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 9121 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 9181 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 9241 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 9301 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 9361 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 9421 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 9481 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 9541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 9661 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 9721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 9781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 9841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 9901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 9961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 10021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 10081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 10141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 10201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 10261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 10321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 10381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 10441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 10501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 10561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 10621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 10681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 10741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 10801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 10861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 10921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 10981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 11041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 11101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 11161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 11221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 11281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 11341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 11401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 11461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 11521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 11581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 11641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 11701 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 11761 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 11821 tccccgaaaa gtgccacctg ac //