LOCUS       Exported               11897 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Derived from pAH-CTX1-rha. The Burkholderia cenocepacia 
            codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is 
            controlled by the rhamnose-inducible promoter system..
ACCESSION   .
VERSION     .
KEYWORDS    pAH-CTX1-rhadCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST
  TITLE     A broad-host-range CRISPRi toolkit for silencing gene expression in 
            Burkholderia.
  JOURNAL   ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232.
  PUBMED    31491085
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(238..285)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     825..845
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      883..910
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      926..997
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1021..5127)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(5151..5269)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             5415..6251
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             6325..7173
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     primer_bind     complement(7219..7235)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7220..7236)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7260..7279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7261..7279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7453..7500)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            7707..7815
                     /note="incP origin of transfer"
     rep_origin      complement(9240..9828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9320..9339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10650..11840)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     11469..11488
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(11888..19)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggag ctccaccgcg gtggcggccg cacgcagaaa ggcccacccg
      901 aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa
      961 aggcccagtc tttcgactga gcctttcgtt tcatttgatg cctggagatc ctcactcgag
     1021 tcagtcacca cccagctgcg agaggtcgat acgcgtttcg tagaggccgg taatgctctg
     1081 gtggatcagc gtggcgtcga gaacctcctt ggtgctggta tagcgcttgc ggtcgatggt
     1141 cgtgtcgaaa tacttgaagg ccgcgggggc gcccaggttg gtcagggtga acaggtggat
     1201 gatgttctcc gcctgttcgc ggatcggctt atcgcggtgt ttgttgtagg cgctcaggac
     1261 tttatccagg tttgcgtccg cgagaatgac gcgcttggag aactccgaga tctgttcaat
     1321 gatctcatcc aggtagtgtt tatgctgttc aacaaagagt tgcttttgct cgttatcctc
     1381 cggcgaaccc ttgagctttt cgtagtggct ggcgaggtac agaaagttga cgtatttgct
     1441 cggcagcgcc agttcgttac ctttttggag ctcacccgcc gacgccagca tgcgcttgcg
     1501 gccattttcg agttcgaaca gcgaatattt cggcagcttg ataatcagat cctttttcac
     1561 ctctttgtag cccttggctt cgaggaagtc gatcgggttt ttctcgaagc tggagcgctc
     1621 cataatggtg atgccgagca gctctttaac cgatttgagt tttttggact tgcccttttc
     1681 caccttcgcg accacgagca ccgaatacgc caccgtgggc gagtcgaagc cgccatactt
     1741 cttgggatcc caatctttct tgcgggcgat gagtttgtcc gagttgcgct tcggcaggat
     1801 gctttccttg gagaaaccgc ccgtctggac ttcggtcttc ttgacgatat tgacttgcgg
     1861 catcgagagc accttgcgca cggtcgcgaa atcgcggccc ttatcccaca cgatttcacc
     1921 cgtttcgccg ttcgtctcga tgagcgggcg tttgcggatc tcgccgttgg cgagcgtaat
     1981 ctcggtttta aagaaattca tgatattcga gtagaagaag tacttggccg tggccttgcc
     2041 gatttcctgt tcgcttttcg caatcatctt ccgcacgtca tacaccttat agtcgccgta
     2101 gacgaattcc gattccagct tgggatattt cttaatcagc gcggtgccga cgaccgcgtt
     2161 gagatatgcg tcgtgtgcgt ggtgatagtt gttaatttcg cgaaccttgt aaaactggaa
     2221 gtctttgcgg aaatccgaga cgagcttcga cttgagggta atgaccttca cttcgcggat
     2281 cagcttatca ttttcgtcgt acttcgtatt catccggctg tcgaggatct gcgcgacgtg
     2341 cttggtgatc tggcgcgttt ccacgagctg gcgcttaatg aagcctgcct tgtccagctc
     2401 gctgaggccg ccgcgttcgg ctttggtcag gttgtcaaac ttgcgctgcg taatgagctt
     2461 cgcgttcagc agctggcgcc agtaattctt catctttttg accacttcct cactcggcac
     2521 gttgtcgctc ttgccgcggt ttttgtcgga gcgggtgagc actttgttgt caatcgagtc
     2581 gtctttgagg aaggactggg gcacgatggc atcgacgtcg taatccgaga gacgattaat
     2641 gtccagctcc tggtcaacat acatgtcgcg gccgttctgc aggtagtaca ggtagagctt
     2701 ttcgttctgc agctgggtat tctcaaccgg gtgctccttg aggatttggc tgcccagttc
     2761 tttgatgcct tcttcaatgc gcttcatgcg ctcgcgggag ttcttctgac ccttctgggt
     2821 cgtctggttc tcgcgggcca tttcgatcac gatgttctcg ggcttatggc gacccatcac
     2881 tttgacgagt tcgtccacaa ctttgacggt ctgcagaatg cccttcttga ttgccgggga
     2941 gcctgccagg ttggcaatat gttcgtggag cgagtcaccc tggcccgaca cctgcgcctt
     3001 ctggatgtcc tccttgaacg tcaggctatc gtcatggatg agctgcatga agttgcggtt
     3061 ggcgaagccg tcggatttca ggaagtcgag aatggtcttg ccgctctgtt tgtcccggat
     3121 gccgttgatc agcttgcgac tcaggcggcc ccagcccgta tagcggcggc gtttgagttg
     3181 tttcatcacc ttgtcgtcga agaggtgggc gtacgtcttc aggcgctcct cgatcatttc
     3241 ccggtcttca aagagcgtca gcgtcaggac gatatcttcc agaatatcct cattctcctc
     3301 gttgtccagg aagtccttat ccttgatgat tttgagcaga tcgtggtagg taccgaggct
     3361 ggcgttgaag cggtcctcca cgcccgaaat ctccacgctg tcgaagcatt cgattttttt
     3421 gaagtagtcc tccttcagct gcttcacggt gactttacgg ttcgtcttga agagcaggtc
     3481 gacaatggcc ttcttctgct cgcccgagag gaaggccggc ttgcgcatgc cctcggtgac
     3541 atacttgacc ttggtgagct cgttgtacac ggtgaagtat tcatagagga gcgagtgctt
     3601 ggggagcacc ttctcgttcg gcaggttctt gtcgaaattc gtcatgcgtt caatgaagct
     3661 ctgggcgctg gcacccttat caaccacttc ttcgaagttc cagggcgtga tggtctcttc
     3721 actcttgcgc gtcatccagg cgaaccggct gttgccgcgc gccagcgggc cgacatagta
     3781 cggaatgcgg aaggtgagaa ttttttcgat tttttcccga ttgtctttca ggaacggata
     3841 gaagtcctcc tggcgccgca ggatggcatg gagttcgccc aggtgaatct ggtgggggat
     3901 gctgccgttg tcgaaggtgc gttgtttgcg cagcagatct tcgcggttca gcttgaccag
     3961 cagctcctcc gtgccgtcca tcttctccag aatcggttta atgaacttgt agaactcttc
     4021 ttgggacgcg ccaccgtcga tatagccggc atagccgttt ttcgactggt cgaagaaaat
     4081 ttccttgtac ttctccggga gttgctggcg aacgagggcc ttcagcaggg tcagatcctg
     4141 gtggtgttcg tcgtagcgct taatcatgct ggccgacagc ggcgccttcg taatctccgt
     4201 gttgacgcgg agaatgtcgg agagcagaat cgcgtcggac aggttcttgg cggcgaggaa
     4261 gagatcggcg tactgatcgc cgatctgggc cagcaggttg tccaggtcgt cgtcgtaggt
     4321 atccttgctc agttgcagct tcgcgtcttc ggccaggtca aagttgctct tgaagttggg
     4381 cgtgaggccc agggacaggg cgatgaggtt gccgaagaga ccgttcttct tctcgcccgg
     4441 gagttgggcg atgaggtttt ccaggcggcg cgacttactg aggcgcgcgc tgaggatcgc
     4501 cttcgcgtcg acgccggacg cattaatggg gttttcttcg aacagctgat tgtacgtctg
     4561 caccagctga atgaagagct tatcgacgtc cgaattatcg gggttcaggt cgccctcgat
     4621 caggaagtga ccgcggaact taatcatgtg ggccagggcg aggtagatga ggcgcaggtc
     4681 cgctttatcg gtggagtcaa ccagtttctt gcggaggtga tagatcgtgg gatacttctc
     4741 atggtacgca acttcgtcga cgatgttacc gaaaatcgga tggcgctcgt gcttcttgtc
     4801 ctcctcgacc aggaagctct cctccagacg gtggaagaag ctatcgtcca ccttggccat
     4861 ttcgttgctg aagatttcct gcaggtagca aatgcgattt ttgcggcgcg tgtagcgccg
     4921 acgcgccgta cgcttgaggc gggtcgcttc ggccgtttcg cccgaatcga acagcagcgc
     4981 gccaatcaga tttttcttaa tggagtggcg gtccgtattg ccgagcactt tgaatttctt
     5041 actcgggacc ttgtattcgt ccgtgatgac cgcccagccc acggaattcg tgccgatcgc
     5101 gaggccgatc gagtacttct tgtccataac tagttcctgc tgaatttcat tacgaccagt
     5161 ctaaaaagcg cctgaattcg cgaccttctc gttactgaca ggaaaatggg ccattggcaa
     5221 ccagggaaag atgaacgtga tgatgttcac aatttgctga attgtggtga tgtgatgctc
     5281 accgcatttc ctgaaaattc acgctgtatc ttgaaaaatc gacgtttttt acgtggtttt
     5341 ccgtcgaaaa tttaaggtaa gaacctgacc tcgtgattac tatttcgccg tgttgacgac
     5401 atcaggaggc cagtatgacc gtattacata gtgtggattt ttttccgtct ggtaacgcgt
     5461 ccgtggcgat agaaccccgg ctcccgcagg cggattttcc tgaacatcat catgattttc
     5521 atgaaattgt gattgtcgaa catggcacgg gtattcatgt gtttaatggg cagccctata
     5581 ccatcaccgg tggcacggtc tgtttcgtac gcgatcatga tcggcatctg tatgaacata
     5641 ccgataatct gtgtctgacc aatgtgctgt atcgctcgcc ggatcgattt cagtttctcg
     5701 ccgggctgaa tcagttgctg ccacaagagc tggatgggca gtatccgtct cactggcgcg
     5761 ttaaccacag cgtattgcag caggtgcgac agctggttgc acagatggaa cagcaggaag
     5821 gggaaaatga tttaccctcg accgccagtc gcgagatctt gtttatgcaa ttactgctct
     5881 tgctgcgtaa aagcagtttg caggagaacc tggaaaacag cgcatcacgt ctcaacttgc
     5941 ttctggcctg gctggaggac cattttgccg atgaggtgaa ttgggatgcc gtggcggatc
     6001 aattttctct ttcactgcgt acgctacatc ggcagcttaa gcagcaaacg ggactgacgc
     6061 ctcagcgata cctgaaccgc ctgcgactga tgaaagcccg acatctgcta cgccacagcg
     6121 aggccagcgt tactgacatc gcctatcgct gtggattcag cgacagtaac cacttttcga
     6181 cgctttttcg ccgagagttt aactggtcac cgcgtgatat tcgccaggga cgggatggct
     6241 ttctgcaata acgcgaatct tctcaacgta tttgtacgcc atattgcgaa taatcaactt
     6301 cgttctctgg ccgaggtagc cacggtggcg catcagttaa aacttctcaa agatgatttt
     6361 tttgccagcg accagcaggc agtcgctgtg gctgaccgtt atccgcaaga tgtctttgct
     6421 gaacatacac atgatttttg tgagctggtg attgtctggc gcggtaatgg cctgcatgta
     6481 ctcaacgatc gcccttatcg cattacccgt ggcgatctct tttacattca tgctgacgat
     6541 aaacactcct acgcttccgt taacgatctg gttttgcaga atattattta ttgcccggag
     6601 cgtctgaagc tgaatcttga ctggcagggg gcgattccgg gatttaacgc cagcgcaggg
     6661 caaccacact ggcgcttagg tagcatgggg atggcgcagg cgcggcaggt tatcggtcag
     6721 cttgagcatg aaagtagtca gcatgtgccg tttgctaacg aaatggctga gttgctgttc
     6781 gggcagttgg tgatgttgct gaatcgccat cgttacacca gtgattcgtt gccgccaaca
     6841 tccagcgaaa cgttgctgga taagctgatt acccggctgg cggctagcct gaaaagtccc
     6901 tttgcgctgg ataaattttg tgatgaggca tcgtgcagtg agcgcgtttt gcgtcagcaa
     6961 tttcgccagc agactggaat gaccatcaat caatatctgc gacaggtcag agtgtgtcat
     7021 gcgcaatatc ttctccagca tagccgcctg ttaatcagtg atatttcgac cgaatgtggc
     7081 tttgaagata gtaactattt ttcggtggtg tttacccggg aaaccgggat gacgcccagc
     7141 cagtggcgtc atctcaattc gcagaaagat taaatgcata tcgatgagct cgcacgaacc
     7201 cagttgacat aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     7261 cctatagtga gtcgtattac gcgcgccggt ggatgacctt ttgaatgacc tttaatagat
     7321 tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa aattttttca
     7381 caaaacggtt tacaagcata aagctaattc cattgagtaa gtttttaagc acatcagctt
     7441 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     7501 aagatcccca attcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7561 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7621 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7681 gcaggaaaga acatgctaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc
     7741 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     7801 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     7861 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     7921 accccgaagc agggttatgc agcggaaagt ataccttaac ctttttcgtt gcttcaggaa
     7981 agtttcagcg cctgattcag cagacctacg atgtcggggc cgtccttgct gatccacttg
     8041 gcgtaatgct tgaagatcat cgccgtcgag gtgtgcccca tctggtccgc gatccattcg
     8101 ggagtggcga tgccgctgct gagcatctgg ctggcgaagg tgtggcggca ctgattggga
     8161 ccacgcgagc ggacgcccgc attcctgagg tggccatgcc accagccgtt gcgcagcgta
     8221 tccgacgtgg agtacgccgc cccgctagca ctgttgtgga acacgaagcg tacccgttga
     8281 ggctttctgg tccggttgtc gcggtctatc acctcgatct gtacgggcgg aaggcgtctg
     8341 gttagcttgg cctgctgctg gagtgcgcgc agcgccgggg ccagtagctg gaccttcctc
     8401 gtcgatcggc gagtcttggt gaccttgtac tggctgcgta cacgagcgcg gcggaaaacc
     8461 acggtccccg tgtccaaatc gacatcttcc caggccagag ctatggcctc gctgacgcgg
     8521 gggccgctcc agatcatgaa ctccgccagg ttcagctcac cgatgcgcgc ggtttcagta
     8581 ccgaggatga ggtcgatttc ctcacgagtg aatgggtctg ggtcatccgc atccggaaga
     8641 gtgatcacga tgccgtctgt tgggtcgtgc gcgctccggt tccgagttcg atacagccgg
     8701 aagatctgcc gcaggttgct gacgatctct cgcaccgtct tgttgtgcag cttgggcatg
     8761 agcgtgttct gcacccagtc ttgaatatcc aggtggtcga tgctgtcggc ctggctgtca
     8821 ccccagcgag gtcggatgtg ggtttccacg cggctggtgt agccgcggaa gccgctggcg
     8881 gcaatctggt tgcgtttgat gtcgagccag aggtcgatgt aatggcccaa ggtgttgctt
     8941 ttcacccgcg gtgagtccgg gaagtgtcgg ctgtagctga agactccttg cttgatctcg
     9001 tagttgatga tgccggccag ccgttcggca ttggctatat tctcgggcgt tgcgtcccct
     9061 ggtatggact ccctacacag ctcgccctgg tagcggaagt agatcctgat ccgttttccg
     9121 cgtacctcga ctccgtctgc catctggttc ccctgatcca caccaagcgt tcaagctggc
     9181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9661 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9961 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10021 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10081 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10141 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10201 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10261 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10321 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10381 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10441 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10501 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtcaagggtt
    10561 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
    10621 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
    10681 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
    10741 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
    10801 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
    10861 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
    10921 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
    10981 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
    11041 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
    11101 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
    11161 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
    11221 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcggcgctct
    11281 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
    11341 gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc ggccacgggg
    11401 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
    11461 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
    11521 ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc catgatcgcg
    11581 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
    11641 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
    11701 acgccatagt gactggcgat gctgtcggaa tggacaatat cccgcaagag gcccggcagt
    11761 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
    11821 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
    11881 ctaccgcatt aaagcta
//
