LOCUS Exported 6710 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses TRAIL receptor 2. ACCESSION . VERSION . KEYWORDS pcDNA3-TRAIL-R2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6710) AUTHORS Clancy L, Mruk K, Archer K, Woelfel M, Mongkolsapaya J, Screaton G, Lenardo MJ, Chan FK TITLE Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis. JOURNAL Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18099-104. Epub 2005 Nov 30. PUBMED 16319225 REFERENCE 2 (bases 1 to 6710) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6710 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(2029..2047) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2030..2047) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2067..2084) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2073..2297 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2343..2771 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2430..2449) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2640..2661 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2780..2800) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2785..3114 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2965..3100 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3027..3046 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3181..3975 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3235..3254) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3845..3864 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4149..4270 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4186..4205) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4240..4259 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4319..4335) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4319..4335) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4332..4354) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4343..4359 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4367..4397) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4412..4433 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4550..4567) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4721..5309) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4801..4820) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5480..6340) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6103..6122 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6341..6445) /gene="bla" /label=AmpR promoter primer_bind complement(6520..6539) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt 661 ggtaccgagc tcggatccgc catggaacaa cggggacaga acgccccggc cgcttcgggg 721 gcccggaaaa ggcacggccc aggacccagg gaggcgcggg gagccaggcc tgggccccgg 781 gtccccaaga cccttgtgct cgttgtcgcc gcggtcctgc tgttggtctc agctgagtct 841 gctctgatca cccaacaaga cctagctccc cagcagagag cggccccaca acaaaagagg 901 tccagcccct cagagggatt gtgtccacct ggacaccata tctcagaaga cggtagagat 961 tgcatctcct gcaaatatgg acaggactat agcactcact ggaatgacct ccttttctgc 1021 ttgcgctgca ccaggtgtga ttcaggtgaa gtggagctaa gtccctgcac cacgaccaga 1081 aacacagtgt gtcagtgcga agaaggcacc ttccgggaag aagattctcc tgagatgtgc 1141 cggaagtgcc gcacagggtg tcccagaggg atggtcaagg tcggtgattg tacaccctgg 1201 agtgacatcg aatgtgtcca caaagaatca ggtacaaagc acagtgggga agccccagct 1261 gtggaggaga cggtgacctc cagcccaggg actcctgcct ctccctgttc tctctcaggc 1321 atcatcatag gagtcacagt tgcagccgta gtcttgattg tggctgtgtt tgtttgcaag 1381 tctttactgt ggaagaaagt ccttccttac ctgaaaggca tctgctcagg tggtggtggg 1441 gaccctgagc gtgtggacag aagctcacaa cgacctgggg ctgaggacaa tgtcctcaat 1501 gagatcgtga gtatcttgca gcccacccag gtccctgagc aggaaatgga agtccaggag 1561 ccagcagagc caacaggtgt caacatgttg tcccccgggg agtcagagca tctgctggaa 1621 ccggcagaag ctgaaaggtc tcagaggagg aggctgctgg ttccagcaaa tgaaggtgat 1681 cccactgaga ctctgagaca gtgcttcgat gactttgcag acttggtgcc ctttgactcc 1741 tgggagccgc tcatgaggaa gttgggcctc atggacaatg agataaaggt ggctaaagct 1801 gaggcagcgg gccacaggga caccttgtac acgatgctga taaagtgggt caacaaaacc 1861 gggcgagatg cctctgtcca caccctgctg gatgccttgg agacgctggg agagagactt 1921 gccaagcaga agattgagga ccacttgttg agctctggaa agttcatgta tctagaaggt 1981 aatgcagact ctgccatgtc cctcgagcat gcatctagag ggccctattc tatagtgtca 2041 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 2101 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 2161 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 2221 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 2281 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 2341 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 2401 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 2461 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 2521 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 2581 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 2641 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 2701 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 2761 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 2821 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 2881 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 2941 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 3001 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct 3061 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc 3121 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc 3181 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 3241 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 3301 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 3361 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 3421 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 3481 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 3541 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 3601 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 3661 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac 3721 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 3781 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 3841 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 3901 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 3961 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 4021 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 4081 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 4141 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 4201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 4261 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 4321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4381 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4441 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 4501 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 4561 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 4621 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 4681 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4741 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 4801 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4861 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 4921 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4981 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 5041 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 5101 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 5161 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 5221 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 5281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 5821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 6061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 6121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 6481 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc 6541 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag 6601 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag 6661 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt //