LOCUS       Exported                3267 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    MTK3_038
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1632..1666
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1737..2396)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2311..2330
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2578..3166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3067..3086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgggcagac gcagacgggg agccattagt gccgaagtgt atactgaaga
       61 ggacgccgcc agctacgtgc ggaaagtgat ccccaaggac tataaaacta tggcagccct
      121 cgctaaggca atcgagaaga acgttttgtt cagtcacctc gacgacaacg aaaggtcaga
      181 catattcgac gctatgttct ctgtatcttt cattgccggg gaaaccgtta tccaacaggg
      241 agatgagggc gacaatttct acgtaataga ccagggcgaa acagacgttt acgtgaataa
      301 cgagtgggct acatccgtag gagagggtgg ctctttcggc gagctggccc tgatctacgg
      361 cactcccagg gccgctaccg ttaaggctaa accagttgta agcgaaagaa tgtatcctga
      421 agatggggct ctcaaaagtg aaataaagaa aggcttgcgg ctcaaagatg gcggacatta
      481 tgcagcagaa gtgaaaacca catataaggc aaagaaacca gttcagttgc cgggagctta
      541 tatagtggat attaaactcg atatagtcag tcataacgaa gattatacaa tagttgagca
      601 atgtgagaga gcagaaggaa ggcattctac tggcggtatg gatgaactct ataaaggtgg
      661 caccggcggt tcattggtca gtaagggaga agaagacaat agagctattg tgaaagaatt
      721 tatgcgattt aaactccata tggaagggag tgtcaatggt catgaatttg aaatagaagg
      781 tgaaggggaa ggtcggccat atgaagcgtt ccaaactgcg aaactcaaag ttacgaaagg
      841 cggacctttg ccatttgctt gggatattct ctctccccaa cttatgtatg gaagtaaagc
      901 atatatcaaa catcccgctg atattccgga ttattttaaa ctcagttttc cagaaggatt
      961 tagatgggaa cgggttatga attttgaaga tggcgggatc atacatgtga gtcaagatac
     1021 aagtctccaa gatggtgtgt ttatatataa agtcaaactt cggggaacaa attttccccc
     1081 agatgggcca gtcatgcaaa agaaaacaat ggggtgggaa gccaccaacg ttaagctttg
     1141 gggtattgat agggattctt accgacggat attgatgggt tcaactctca ggaaacgcaa
     1201 aatgtacgaa gagtttctgt ccaaggtgtc aatccttgaa agcctcgata aatgggagcg
     1261 actgactgtg gcagacgctc ttgagcccgt tcaattcgag gacggtcaga aaatcgtcgt
     1321 acaaggggag ccgggtgacg aattctttat catcttggaa ggcagtgcgg ccgttctcca
     1381 gaggagatct gagaacgagg aattcgttga ggtcggaagg ctcggtccta gcgactactt
     1441 tggcgagatc gccctgctca tgaaccgccc ccgagccgct accgtggtgg caaggggtcc
     1501 actcaaatgt gtgaaacttg ataggccccg gttcgagaga gtgctgggac cttgttccga
     1561 tattctgaag aggaatattc aacaatataa ttccttcgtt agtctctccg ttggatcctg
     1621 agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1681 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     1741 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1801 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1861 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1921 tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca
     1981 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     2041 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     2101 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     2161 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     2221 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     2281 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     2341 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     2401 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2461 tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta
     2521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2941 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3001 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3061 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3121 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3181 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3241 atcccctgat tctgtggata accgtag
//
