LOCUS Exported 4421 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express GABAR-alpha-1 N terminal domain. ACCESSION . VERSION . KEYWORDS pCMV-GABAR-alpha-1N-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4421) AUTHORS Meng X, McGraw CM, Wang W, Jing J, Yeh SY, Wang L, Lopez J, Brown AM, Lin T, Chen W, Xue M, Sillitoe RV, Jiang X, Zoghbi HY TITLE Neurexophilin4 is a selectively expressed alpha-neurexin ligand that modulates specific cerebellar synapses and motor functions. JOURNAL Elife. 2019 Sep 16;8. pii: 46773. doi: 10.7554/eLife.46773. PUBMED 31524598 REFERENCE 2 (bases 1 to 4421) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4421 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 44..347 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 348..551 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 502..522 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 548..572 /label=LNCX /note="Human CMV promoter, forward primer" intron 689..785 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" CDS 1536..1562 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" polyA_signal 1577..1711 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1601..1620) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1655..1674 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1822..1838) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1822..1838) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1835..1857) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1846..1862 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1870..1900) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1915..1936 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2053..2070) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2224..2812) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2304..2323) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2983..3843) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3606..3625 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3844..3948) /gene="bla" /label=AmpR promoter primer_bind 4016..4034 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4072..4094) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4194..4213 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4407..8 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4421..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg 61 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 361 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 481 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 601 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag 661 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg 721 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact 781 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc 841 ccaggcccga attcatgctc gaggatgaac ttaaagacaa caccactgtc ttcacaagaa 901 ttttggaccg actgctggac ggttatgaca atcgtctgag accaggtttg ggagagcgtg 961 taactgaagt gaagaccgac attttcgtca ccagtttcgg accagtttca gaccacgata 1021 tggagtatac aatagatgtg tttttccgtc aaagttggaa ggatgaaaga ttaaaattca 1081 aaggacccat gacagtgctc cggctaaaca accttatggc cagtaaaatc tggactccag 1141 atacattttt ccacaatgga aagaagtctg tggcccacaa catgaccatg cctaataagc 1201 tcctgcgtat cacagaggat ggcactctgc tgtacaccat gaggttgacc gtgagagctg 1261 aatgcccaat gcacttggaa gacttcccta tggatgccca tgcctgccca ctaaaattcg 1321 gaagctatgc ttatacaaga gcagaagttg tctatgagtg gaccagagag ccagcccgtt 1381 cagtggttgt agcagaagat gggtcacgtt taaaccagta tgaccttctt ggacaaacag 1441 ttgactctgg aattgttcag tccagtactg gagaatatgt ggttatgacg actcacttcc 1501 acttgaagag aaaaattggc tctcgaggta ccggctaccc atacgatgtt ccagattacg 1561 cttaagcggc cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac 1621 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 1681 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 1741 tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga tctgcaggca 1801 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1861 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 1921 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 1981 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2041 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2101 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2161 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 2701 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2821 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2881 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2941 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3001 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3061 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 3121 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 3181 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 3241 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 3301 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 3361 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 3421 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 3481 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 3541 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 3601 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 3661 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3721 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3781 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3841 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3901 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3961 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 4021 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 4081 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 4141 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 4201 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 4261 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 4321 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 4381 attaagttgg gtaacgccag ggttttccca gtcacgacgt t //