LOCUS Exported 6116 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication. ACCESSION . VERSION . KEYWORDS pMS82 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6116) AUTHORS Gregory MA, Till R, Smith MC TITLE Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors. JOURNAL J Bacteriol. 2003 Sep;185(17):5320-3. PUBMED 12923110 REFERENCE 2 (bases 1 to 6116) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6116 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(38..54) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(38..54) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(51..73) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 62..78 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(86..116) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 131..152 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(269..286) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(440..1028) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(520..539) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3416..3787) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 3820..3929 /note="incP origin of transfer" ORIGIN 1 gatccgcggc cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa 61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 841 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg 1141 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca 1201 acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt 1261 gcaatacgaa tggcgaaaag ccgagaacct aggatccaag cttagatcta tgcatggtac 1321 cagtgagcgt ttttcaacct caggctgacg gggtcagggg tcagaggcga ggtaaccatc 1381 tgcggtgata aattatctct ggcggtgttg acataaatac cactggcggt gatactgagc 1441 acatcagatc ggtcgtcgag gtccacaggc cctcggcgac gttccgccgg gcctcggcga 1501 ccgccgcgtc gaggcgccgg tcgaaggggc agtcctccac gggcagctcg tggagggcgc 1561 gggccagctc cgccatcgcc tcgaccacgg cgaaccgctg gtgctcgggc cactcctcgg 1621 ccgccgcgac gccggggacg gcctccgtga cgagccacgc ggcggtgtcg tcggcaccgc 1681 gctcgacgac gcgggggacg gggatcggcg gggcctggga cggcgcctcg ccgtcgcaga 1741 accaggcggt ggcgtacacc gtcgcctcgg tcggcccgta gagattggcg atcccgaccg 1801 cagcaccacc gagaacgtcc ccgacgtggc cgaccagccc gtcatcgtca acgcctgatc 1861 cgcggtgcgg acaggccgtg tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg 1921 tgaatagagg tccgctgtga cacaagaatc cctgttactt ctcgaccgta ttgattcgga 1981 tgattcctac gcgagcctgc ggaacgacca ggaattctgg gagccgctgg cccgccgagc 2041 cctggaggag ctcgggctgc cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa 2101 ccccgtactg gtcggcgagc ccggcccggt gatcaagctg ttcggcgagc actggtgcgg 2161 tccggagagc ctcgcgtcgg agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc 2221 ggtgccccgc ctcctcggcc gcggcgagct gcggcccggc accggagcct ggccgtggcc 2281 ctacctggtg atgagccgga tgaccggcac cacctggcgg tccgcgatgg acggcacgac 2341 cgaccggaac gcgctgctcg ccctggcccg cgaactcggc cgggtgctcg gccggctgca 2401 cagggtgccg ctgaccggga acaccgtgct caccccccat tccgaggtct tcccggaact 2461 gctgcgggaa cgccgcgcgg cgaccgtcga ggaccaccgc gggtggggct acctctcgcc 2521 ccggctgctg gaccgcctgg aggactggct gccggacgtg gacacgctgc tggccggccg 2581 cgaaccccgg ttcgtccacg gcgacctgca cgggaccaac atcttcgtgg acctggccgc 2641 gaccgaggtc accgggatcg tcgacttcac cgacgtctat gcgggagact cccgctacag 2701 cctggtgcaa ctgcatctca acgccttccg gggcgaccgc gagatcctgg ccgcgctgct 2761 cgacggggcg cagtggaagc ggaccgagga cttcgcccgc gaactgctcg ccttcacctt 2821 cctgcacgac ttcgaggtgt tcgaggagac cccgctggat ctctccggct tcaccgatcc 2881 ggaggaactg gcgcagttcc tctgggggcc gccggacacc gcccccggcg cctgacgccc 2941 cgggtgaaga gccccgtcgg gcggtgcctg acggggcttc tcagttctca tgagcggaga 3001 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat 3061 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc 3121 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg 3181 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg 3241 tatcgggccc tggccagcta gctagagtcg acctgcaggt ccccggggat cggtcttgcc 3301 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat 3361 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg 3421 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt 3481 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt 3541 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca 3601 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca 3661 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt 3721 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat 3781 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag 3841 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg 3901 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt 3961 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa 4021 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag 4081 gcatgctggc gccggacggg gcttcagacg tttcgggtgc tgggttgttg tctctggaca 4141 gtgatccatg ggaaactact cagcaccacc aatgttccca aaagaaagcg caggtcagcg 4201 cccatgagcc aagatctagg catgtcgccc ttcatcgctc ccgacgtccc tgagcacctt 4261 ctagacactg ttcgcgtctt cctgtacgcg cgtcagtcta agggccggtc cgacggctca 4321 gacgtgtcga ccgaagcaca gctagcggcc ggtcgtgcgt tggtcgcgtc tcgcaacgcc 4381 caggggggtg cgcgctgggt cgtggcaggt gagttcgtgg acgtcgggcg ctccggctgg 4441 gacccgaacg tgacccgtgc cgacttcgag cgcatgatgg gcgaagtccg cgccggcgaa 4501 ggtgacgttg tcgttgtgaa tgagctttcc cggctcactc gcaagggcgc ccatgacgcg 4561 ctcgaaatcg acaacgaatt gaagaagcac ggcgtgcgct tcatgtcggt tcttgagccg 4621 ttccttgaca cgtctacccc tatcggcgtc gccattttcg cgctgatcgc tgcccttgcg 4681 aaacaggaca gtgacctgaa ggcggagcgc ctgaagggtg cgaaagacga gattgccgcg 4741 ctgggtggcg ttcactcgtc ttccgccccg ttcggaatgc gcgccgtgcg caagaaggtc 4801 gataatctcg tgatctccgt tcttgagccg gacgaagaca acccggatca cgtcgagcta 4861 gttgagcgca tggcgaaaat gtcgttcgaa ggcgtgtccg acaacgccat tgcaacgacc 4921 ttcgagaagg aaaagatccc gtcgcccgga atggctgaga gacgcgccac ggaaaagcgt 4981 cttgcgtcca tcaaggcacg tcgcctgaac ggcgctgaaa agccgatcat gtggcgcgct 5041 caaacggtcc gatggattct caaccatccc gcaatcggcg gtttcgcatt cgagcgtgtg 5101 aagcacggta aggcgcacat caacgtcata cggcgcgacc ccggcggcaa gccgctaacg 5161 ccccacacgg gcattctcag cggctcgaag tggcttgagc ttcaagagaa gcgttccggg 5221 aagaatctca gcgaccggaa gcctggggcc gaagtcgaac cgacgcttct gagcgggtgg 5281 cgtttcctgg ggtgccgaat ctgcggcggc tcaatgggtc agtcccaggg tggccgtaag 5341 cgcaacggcg accttgccga aggcaattac atgtgcgcca acccgaaggg gcacggcggc 5401 ttgtcggtca agcgcagcga actggacgaa ttcgttgctt cgaaggtgtg ggcacggctc 5461 cgcacagccg acatggaaga tgaacacgat caggcatgga ttgccgccgc tgcggagcgc 5521 ttcgcccttc agcacgacct agcgggggtg gccgatgagc ggcgcgaaca acaggcgcac 5581 ctagacaacg tgcggcgctc catcaaggac cttcaggcgg accgtaaggc cggtctgtac 5641 gtcgggcgtg aagagctgga aacgtggcgc tcaacggtgc tgcaataccg gtcctacgaa 5701 gcggagtgca cgacccgact cgctgagctt gacgagaaga tgaacggcag cacccgcgtt 5761 ccgtctgagt ggttcagcgg cgaagacccg acggccgaag ggggcatctg ggcaagctgg 5821 gacgtgtacg agcgtcggga gttcctgagc ttcttccttg actccgtcat ggtcgaccgg 5881 gggcgccacc ctgagacgaa gaaatacatc cccctgaagg accgtgtgac gctcaagtgg 5941 gcggagctgc tgaaggagga agacgaagcg agcgaagcca ctgagcggga gcttgcggcg 6001 ctgtagcgca cagcgggagg ggtcgagccg gcggacggtt cggccccttt tttggccttg 6061 aaatcgttag ttaggctaac tagtagttcc ttcgtcacca tagcgggcag ggagcg //