LOCUS Exported 10957 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of FGFR3 S249C. ACCESSION . VERSION . KEYWORDS pHAGE-FGFR3-S249C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10957) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10957) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10957 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5894..5918) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5971..6012 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6033..6600 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6050..6602 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6194..6211) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6421..6440 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6614..7333 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6614..7330 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6659..6680) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6920..6939) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7267..7288 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7347..7935 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7400..7420) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7818..7829) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8010..8243 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8362..8380) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8448..8552 /gene="bla" /label=AmpR promoter CDS 8553..9413 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8771..8790) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9584..10172 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10073..10092 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10326..10343 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10435..10454 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10583..10604 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10619..10649 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10657..10673 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10662..10684 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10681..10697 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10681..10697 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10747..10766 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgggcgc ccctgcctgc 3481 gccctcgcgc tctgcgtggc cgtggccatc gtggccggcg cctcctcgga gtccttgggg 3541 acggagcagc gcgtcgtggg gcgagcggca gaagtcccgg gcccagagcc cggccagcag 3601 gagcagttgg tcttcggcag cggggatgct gtggagctga gctgtccccc gcccgggggt 3661 ggtcccatgg ggcccactgt ctgggtcaag gatggcacag ggctggtgcc ctcggagcgt 3721 gtcctggtgg ggccccagcg gctgcaggtg ctgaatgcct cccacgagga ctccggggcc 3781 tacagctgcc ggcagcggct cacgcagcgc gtactgtgcc acttcagtgt gcgggtgaca 3841 gacgctccat cctcgggaga tgacgaagac ggggaggacg aggctgagga cacaggtgtg 3901 gacacagggg ccccttactg gacacggccc gagcggatgg acaagaagct gctggccgtg 3961 ccggccgcca acaccgtccg cttccgctgc ccagccgctg gcaaccccac tccctccatc 4021 tcctggctga agaacggcag ggagttccgc ggcgagcacc gcattggagg catcaagctg 4081 cggcatcagc agtggagcct ggtcatggaa agcgtggtgc cctcggaccg cggcaactac 4141 acctgcgtcg tggagaacaa gtttggcagc atccggcaga cgtacacgct ggacgtgctg 4201 gagcgctgcc cgcaccggcc catcctgcag gcggggctgc cggccaacca gacggcggtg 4261 ctgggcagcg acgtggagtt ccactgcaag gtgtacagtg acgcacagcc ccacatccag 4321 tggctcaagc acgtggaggt gaacggcagc aaggtgggcc cggacggcac accctacgtt 4381 accgtgctca agacggcggg cgctaacacc accgacaagg agctagaggt tctctccttg 4441 cacaacgtca cctttgagga cgccggggag tacacctgcc tggcgggcaa ttctattggg 4501 ttttctcatc actctgcgtg gctggtggtg ctgccagccg aggaggagct ggtggaggct 4561 gacgaggcgg gcagtgtgta tgcaggcatc ctcagctacg gggtgggctt cttcctgttc 4621 atcctggtgg tggcggctgt gacgctctgc cgcctgcgca gcccccccaa gaaaggcctg 4681 ggctccccca ccgtgcacaa gatctcccgc ttcccgctca agcgacaggt gtccctggag 4741 tccaacgcgt ccatgagctc caacacacca ctggtgcgca tcgcaaggct gtcctcaggg 4801 gagggcccca cgctggccaa tgtctccgag ctcgagctgc ctgccgaccc caaatgggag 4861 ctgtctcggg cccggctgac cctgggcaag ccccttgggg agggctgctt cggccaggtg 4921 gtcatggcgg aggccatcgg cattgacaag gaccgggccg ccaagcctgt caccgtagcc 4981 gtgaagatgc tgaaagacga tgccactgac aaggacctgt cggacctggt gtctgagatg 5041 gagatgatga agatgatcgg gaaacacaaa aacatcatca acctgctggg cgcctgcacg 5101 cagggcgggc ccctgtacgt gctggtggag tacgcggcca agggtaacct gcgggagttt 5161 ctgcgggcgc ggcggccccc gggcctggac tactccttcg acacctgcaa gccgcccgag 5221 gagcagctca ccttcaagga cctggtgtcc tgtgcctacc aggtggcccg gggcatggag 5281 tacttggcct cccagaagtg catccacagg gacctggctg cccgcaatgt gctggtgacc 5341 gaggacaacg tgatgaagat cgcagacttc gggctggccc gggacgtgca caacctcgac 5401 tactacaaga agacaaccaa cggccggctg cccgtgaagt ggatggcgcc tgaggccttg 5461 tttgaccgag tctacactca ccagagtgac gtctggtcct ttggggtcct gctctgggag 5521 atcttcacgc tggggggctc cccgtacccc ggcatccctg tggaggagct cttcaagctg 5581 ctgaaggagg gccaccgcat ggacaagccc gccaactgca cacacgacct gtacatgatc 5641 atgcgggagt gctggcatgc cgcgccctcc cagaggccca ccttcaagca gctggtggag 5701 gacctggacc gtgtccttac cgtgacgtcc accgacgagt acctggacct gtcggcgcct 5761 ttcgagcagt actccccggg tggccaggac acccccagct ccagctcctc aggggacgac 5821 tccgtgtttg cccacgacct gctgcccccg gccccaccca gcagtggggg ctcgcggacg 5881 tagggcctca tgggcccagc tttcttgtac aaagtggttg atatccagca cagtggcggc 5941 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt 6001 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc 6061 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 6121 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 6181 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 6241 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 6301 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 6361 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 6421 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 6481 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 6541 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 6601 tatggccaca catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 6661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 6721 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 6781 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 6841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 6901 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 6961 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 7021 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 7081 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 7141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 7201 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 7261 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 7321 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat 7381 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 7441 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 7501 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 7561 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 7621 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 7681 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 7741 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 7801 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 7861 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 7921 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct 7981 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 8041 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 8101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 8221 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 8281 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag 8341 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 8401 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 8461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 8521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 8581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 8641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 8701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 8761 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 8821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 8881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 8941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 9001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 9061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 9121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 9181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 9241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 9301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 9361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 9421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 9481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 9541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 9601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 9661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 9721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 9781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 9841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 9901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 9961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 10021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 10081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 10141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 10201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 10261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 10321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 10381 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta 10441 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 10501 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag 10561 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 10621 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 10681 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt 10741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 10801 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 10861 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa 10921 attgtatttg ttaaatatgt actacaaact tagtagt //