LOCUS Exported 8518 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABE-puro-DN-hTERT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8518) AUTHORS Hahn WC, Stewart SA, Brooks MW, York SG, Eaton E, Kurachi A, Beijersbergen RL, Knoll JH, Meyerson M, Weinberg RA TITLE Inhibition of telomerase limits the growth of human cancer cells. JOURNAL Nat Med 1999 Oct;5(10):1164-70. PUBMED 10502820 REFERENCE 2 (bases 1 to 8518) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8518 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(2..21) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(670..1530) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1293..1312 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1531..1635) /gene="bla" /label=AmpR promoter LTR 1666..2135 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2200..2399 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2600..3016 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 2946..2968 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 2984..3000 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS complement(6144..6155) /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" primer_bind complement(6506..6526) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6511..6840 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6691..6826 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6753..6772 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6850..7449 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6850..6869) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7346..7366 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7670..8139 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(8269..8286) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8440..510) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 601 atcttcacct agatcctttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg 661 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 721 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 781 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 841 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 901 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 961 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1021 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1081 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1141 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1201 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1261 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1321 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1381 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 1441 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 1501 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 1561 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 1621 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa tatgggccaa 1681 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag 1741 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 1801 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt 1861 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt 1921 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac 1981 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa 2041 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc 2101 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga 2161 gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag caacttatct 2221 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt 2281 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg 2341 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg 2401 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa 2461 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc 2521 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct 2581 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca 2641 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg 2701 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac 2761 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg 2821 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc 2881 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc 2941 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac 3001 tccttctcta ggcgccggcc ggatcccagt gtggtggtac gtaggaattc gtcctgctgc 3061 gcacgtggga agccctggcc ccggccaccc ccgcgatgcc gcgcgctccc cgctgccgag 3121 ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct gccgctggcc acgttcgtgc 3181 ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg ggacccggcg gctttccgcg 3241 cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc acggccgccc cccgccgccc 3301 cctccttccg ccaggtgtcc tgcctgaagg agctggtggc ccgagtgctg cagaggctgt 3361 gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc gctgctggac ggggcccgcg 3421 ggggcccccc cgaggccttc accaccagcg tgcgcagcta cctgcccaac acggtgaccg 3481 acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg ccgcgtgggc gacgacgtgc 3541 tggttcacct gctggcacgc tgcgcgctct ttgtgctggt ggctcccagc tgcgcctacc 3601 aggtgtgcgg gccgccgctg taccagctcg gcgctgccac tcaggcccgg cccccgccac 3661 acgctagtgg accccgaagg cgtctgggat gcgaacgggc ctggaaccat agcgtcaggg 3721 aggccggggt ccccctgggc ctgccagccc cgggtgcgag gaggcgcggg ggcagtgcca 3781 gccgaagtct gccgttgccc aagaggccca ggcgtggcgc tgcccctgag ccggagcgga 3841 cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac gcgtggaccg agtgaccgtg 3901 gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc cacctctttg gagggtgcgc 3961 tctctggcac gcgccactcc cacccatccg tgggccgcca gcaccacgcg ggccccccat 4021 ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc cccggtgtac gccgagacca 4081 agcacttcct ctactcctca ggcgacaagg agcagctgcg gccctccttc ctactcagct 4141 ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga gaccatcttt ctgggttcca 4201 ggccctggat gccagggact ccccgcaggt tgccccgcct gccccagcgc tactggcaaa 4261 tgcggcccct gtttctggag ctgcttggga accacgcgca gtgcccctac ggggtgctcc 4321 tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc agccggtgtc tgtgcccggg 4381 agaagcccca gggctctgtg gcggcccccg aggaggagga cacagacccc cgtcgcctgg 4441 tgcagctgct ccgccagcac agcagcccct ggcaggtgta cggcttcgtg cgggcctgcc 4501 tgcgccggct ggtgccccca ggcctctggg gctccaggca caacgaacgc cgcttcctca 4561 ggaacaccaa gaagttcatc tccctgggga agcatgccaa gctctcgctg caggagctga 4621 cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag gagcccaggg gttggctgtg 4681 ttccggccgc agagcaccgt ctgcgtgagg agatcctggc caagttcctg cactggctga 4741 tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta tgtcacggag accacgtttc 4801 aaaagaacag gctctttttc taccggaaga gtgtctggag caagttgcaa agcattggaa 4861 tcagacagca cttgaagagg gtgcagctgc gggagctgtc ggaagcagag gtcaggcagc 4921 atcgggaagc caggcccgcc ctgctgacgt ccagactccg cttcatcccc aagcctgacg 4981 ggctgcggcc gattgtgaac atggactacg tcgtgggagc cagaacgttc cgcagagaaa 5041 agagggccga gcgtctcacc tcgagggtga aggcactgtt cagcgtgctc aactacgagc 5101 gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg cctggacgat atccacaggg 5161 cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc gccgcctgag ctgtactttg 5221 tcaaggtcgc gatcacgggc gcgtacgaca ccatccccca ggacaggctc acggaggtca 5281 tcgccagcat catcaaaccc cagaacacgt actgcgtgcg tcggtatgcc gtggtccaga 5341 aggccgccca tgggcacgtc cgcaaggcct tcaagagcca cgtctctacc ttgacagacc 5401 tccagccgta catgcgacag ttcgtggctc acctgcagga gaccagcccg ctgagggatg 5461 ccgtcgtcat cgagcagagc tcctccctga atgaggccag cagtggcctc ttcgacgtct 5521 tcctacgctt catgtgccac cacgccgtgc gcatcagggg caagtcctac gtccagtgcc 5581 aggggatccc gcagggctcc atcctctcca cgctgctctg cagcctgtgc tacggcgaca 5641 tggagaacaa gctgtttgcg gggattcggc gggacgggct gctcctgcgt ttggtggatg 5701 atttcttgtt ggtgacacct cacctcaccc acgcgaaaac cttcctcagg accctggtcc 5761 gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa gacagtggtg aacttccctg 5821 tagaagacga ggccctgggt ggcacggctt ttgttcagat gccggcccac ggcctattcc 5881 cctggtgcgg cctgctgctg gatacccgga ccctggaggt gcagagcgac tactccagct 5941 atgcccggac ctccatcaga gccagtctca ccttcaaccg cggcttcaag gctgggagga 6001 acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg tcacagcctg tttctggatt 6061 tgcaggtgaa cagcctccag acggtgtgca ccaacatcta caagatcctc ctgctgcagg 6121 cgtacaggtt tcacgcatgt gtgctgcagc tcccatttca tcagcaagtt tggaagaacc 6181 ccacattttt cctgcgcgtc atctctgaca cggcctccct ctgctactcc atcctgaaag 6241 ccaagaacgc agggatgtcg ctgggggcca agggcgccgc cggccctctg ccctccgagg 6301 ccgtgcagtg gctgtgccac caagcattcc tgctcaagct gactcgacac cgtgtcacct 6361 acgtgccact cctggggtca ctcaggacag cccagacgca gctgagtcgg aagctcccgg 6421 ggacgacgct gactgccctg gaggccgcag ccaacccggc actgccctca gacttcaaga 6481 ccatcctgga ctgagtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 6541 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 6601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 6661 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 6721 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6781 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 6841 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 6901 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 6961 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 7021 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 7081 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 7141 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 7201 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 7261 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 7321 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 7381 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 7441 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat 7501 gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc 7561 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca 7621 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg 7681 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga 7741 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 7801 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag 7861 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat 7921 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca 7981 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat 8041 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct 8101 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt 8161 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg 8221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 8281 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 8341 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 8401 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 8461 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggacta //