LOCUS Exported 9952 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of AKT3 S370P. ACCESSION . VERSION . KEYWORDS pHAGE-AKT3-S370P SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9952) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9952) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9952 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..118 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 166..1344 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 397..1335 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1292..1312 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1364..1388 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2832..2856) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2909..2950 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2971..3538 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2988..3540 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3132..3149) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3359..3378 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3552..4271 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3552..4268 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3597..3618) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3858..3877) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4205..4226 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4285..4873 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4338..4358) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4756..4767) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4948..5181 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(5300..5318) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5386..5490 /gene="bla" /label=AmpR promoter CDS 5491..6351 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5709..5728) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6522..7110 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7011..7030 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7264..7281 /label=L4440 /note="L4440 vector, forward primer" primer_bind 7373..7392 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 7521..7542 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7557..7587 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7595..7611 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7600..7622 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7619..7635 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7619..7635 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7685..7704 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 7896..8529 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8576..8701 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9198..9431 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9616..9660 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt 181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg 481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct 541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc 601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta 661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta 721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg 781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga 841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg 901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa 961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga 1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct 1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt 1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa 1381 agcaggcacc atgagcgatg ttaccattgt gaaagaaggt tgggttcaga agaggggaga 1441 atatataaaa aactggaggc caagatactt ccttttgaag acagatggct cattcatagg 1501 atataaagag aaacctcaag atgtggattt accttatccc ctcaacaact tttcagtggc 1561 aaaatgccag ttaatgaaaa cagaacgacc aaagccaaac acatttataa tcagatgtct 1621 ccagtggact actgttatag agagaacatt tcatgtagat actccagagg aaagggaaga 1681 atggacagaa gctatccagg ctgtagcaga cagactgcag aggcaagaag aggagagaat 1741 gaattgtagt ccaacttcac aaattgataa tataggagag gaagagatgg atgcctctac 1801 aacccatcat aaaagaaaga caatgaatga ttttgactat ttgaaactac taggtaaagg 1861 cacttttggg aaagttattt tggttcgaga gaaggcaagt ggaaaatact atgctatgaa 1921 gattctgaag aaagaagtca ttattgcaaa ggatgaagtg gcacacactc taactgaaag 1981 cagagtatta aagaacacta gacatccctt tttaacatcc ttgaaatatt ccttccagac 2041 aaaagaccgt ttgtgttttg tgatggaata tgttaatggg ggcgagctgt ttttccattt 2101 gtcgagagag cgggtgttct ctgaggaccg cacacgtttc tatggtgcag aaattgtctc 2161 tgccttggac tatctacatt ccggaaagat tgtgtaccgt gatctcaagt tggagaatct 2221 aatgctggac aaagatggcc acataaaaat tacagatttt ggactttgca aagaagggat 2281 cacagatgca gccaccatga agacattctg tggcactcca gaatatctgg caccagaggt 2341 gttagaagat aatgactatg gccgagcagt agactggtgg ggcctagggg ttgtcatgta 2401 tgaaatgatg tgtgggaggt tacctttcta caaccaggac catgagaaac tttttgaatt 2461 aatattaatg gaagacatta aatttcctcg aacactccct tcagatgcaa aatcattgct 2521 ttcagggctc ttgataaagg atccaaataa acgccttggt ggaggaccag atgatgcaaa 2581 agaaattatg agacacagtt tcttctctgg agtaaactgg caagatgtat atgataaaaa 2641 gcttgtacct ccttttaaac ctcaagtaac atctgagaca gatactagat attttgatga 2701 agaatttaca gctcagacta ttacaataac accacctgaa aaatatgatg aggatggtat 2761 ggactgcatg gacaatgaga ggcggccgca tttccctcaa ttttcctact ctgcaagtgg 2821 acgagaatag aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg 2881 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct 2941 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg 3001 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc 3061 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag 3121 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt 3181 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa 3241 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc 3301 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg 3361 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat 3421 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa 3481 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata 3541 tggccacaca tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 3601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 3661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 3721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 3781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 3841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 3901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 3961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 4021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 4081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 4141 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 4201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 4261 tgtacaagta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg 4321 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 4381 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 4441 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 4501 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 4561 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 4621 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 4681 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 4741 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 4801 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 4861 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta 4921 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 4981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 5041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 5101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 5161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 5221 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat 5281 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 5341 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5461 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 5521 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 5581 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 5641 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 5701 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt 5761 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 5821 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 5881 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 5941 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 6001 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 6061 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 6121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 6181 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 6241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 6301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 6361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 6421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 6481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 6541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 6901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 6961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 7021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 7081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 7141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 7201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 7261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 7321 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg 7381 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 7441 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg 7501 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 7561 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 7621 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct 7681 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 7741 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 7801 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 7861 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag 7921 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca 7981 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct 8041 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt 8101 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt 8161 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa 8221 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg 8281 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg 8341 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 8401 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 8461 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 8521 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc 8581 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 8641 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 8701 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 8761 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 8821 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 8881 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 8941 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 9001 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga 9061 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 9121 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 9181 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 9241 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 9301 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 9361 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 9421 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 9481 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg 9541 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg 9601 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg 9661 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta 9721 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg 9781 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg 9841 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg 9901 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aa //