LOCUS Exported 12094 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of EGFR R521K. ACCESSION . VERSION . KEYWORDS pHAGE-EGFR-R521K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12094) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 12094) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12094 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7054..7078) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7086..7104 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7129..7148) /label=T7 /note="T7 promoter, forward primer" promoter complement(7130..7148) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7154..7174) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7228..7269 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7290..7857 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7307..7859 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7451..7468) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7678..7697 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7871..8470 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7871..7890) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8367..8387 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8484..9072 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8537..8557) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8955..8966) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9147..9380 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9499..9517) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9585..9689 /gene="bla" /label=AmpR promoter CDS 9690..10550 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9908..9927) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10721..11309 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11210..11229 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11463..11480 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11572..11591 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11720..11741 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11756..11786 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11794..11810 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11799..11821 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11818..11834 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11818..11834 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11884..11903 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc 3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc 3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc 3601 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag 3661 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt 3721 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg 3781 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga 3841 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc 3901 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac 3961 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat 4021 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc 4081 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc 4141 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc 4201 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac 4261 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc 4321 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt 4381 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg 4441 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata 4501 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc 4561 ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa 4621 ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 4681 gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag 4741 caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc 4801 tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat 4861 gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata 4921 agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 4981 cccgagggct gctggggccc ggagcccaag gactgcgtct cttgccggaa tgtcagccga 5041 ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag 5101 aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc 5161 acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc 5221 gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 5281 gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca 5341 ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg 5401 ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc 5461 cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct tgtggagcct 5521 cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa 5581 ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca cggtgtataa gggactctgg 5641 atcccagaag gtgagaaagt taaaattccc gtcgctatca aggaattaag agaagcaaca 5701 tctccgaaag ccaacaagga aatcctcgat gaagcctacg tgatggccag cgtggacaac 5761 ccccacgtgt gccgcctgct gggcatctgc ctcacctcca ccgtgcagct catcacgcag 5821 ctcatgccct tcggctgcct cctggactat gtccgggaac acaaagacaa tattggctcc 5881 cagtacctgc tcaactggtg tgtgcagatc gcaaagggca tgaactactt ggaggaccgt 5941 cgcttggtgc accgcgacct ggcagccagg aacgtactgg tgaaaacacc gcagcatgtc 6001 aagatcacag attttgggct ggccaaactg ctgggtgcgg aagagaaaga ataccatgca 6061 gaaggaggca aagtgcctat caagtggatg gcattggaat caattttaca cagaatctat 6121 acccaccaga gtgatgtctg gagctacggg gtgaccgttt gggagttgat gacctttgga 6181 tccaagccat atgacggaat ccctgccagc gagatctcct ccatcctgga gaaaggagaa 6241 cgcctccctc agccacccat atgtaccatc gatgtctaca tgatcatggt caagtgctgg 6301 atgatagacg cagatagtcg cccaaagttc cgtgagttga tcatcgaatt ctccaaaatg 6361 gcccgagacc cccagcgcta ccttgtcatt cagggggatg aaagaatgca tttgccaagt 6421 cctacagact ccaacttcta ccgtgccctg atggatgaag aagacatgga cgacgtggtg 6481 gatgccgacg agtacctcat cccacagcag ggcttcttca gcagcccctc cacgtcacgg 6541 actcccctcc tgagctctct gagtgcaacc agcaacaatt ccaccgtggc ttgcattgat 6601 agaaatgggc tgcaaagctg tcccatcaag gaagacagct tcttgcagcg atacagctca 6661 gaccccacag gcgccttgac tgaggacagc atagacgaca ccttcctccc agtgcctgaa 6721 tacataaacc agtccgttcc caaaaggccc gctggctctg tgcagaatcc tgtctatcac 6781 aatcagcctc tgaaccccgc gcccagcaga gacccacact accaggaccc ccacagcact 6841 gcagtgggca accccgagta tctcaacact gtccagccca cctgtgtcaa cagcacattc 6901 gacagccctg cccactgggc ccagaaaggc agccaccaaa ttagcctgga caaccctgac 6961 taccagcagg acttctttcc caaggaagcc aagccaaatg gcatctttaa gggctccaca 7021 gctgaaaatg cagaatacct aagggtcgca tggtgcctgc tttcttgtac aaagtggtga 7081 tatccaatta accctcacta aaggaagatg atagttaagt gtaagttacc ctatagtgag 7141 tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc 7201 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc 7261 gattctacgc gtaccggtta gtaatgagat ccctnccccc cccctaacgt tactggccga 7321 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 7381 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 7441 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 7501 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 7561 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 7621 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 7681 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 7741 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 7801 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 7861 ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 7921 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 7981 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 8041 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 8101 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 8161 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 8221 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 8281 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 8341 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 8401 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 8461 cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga 8521 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 8581 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 8641 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 8701 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 8761 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 8821 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 8881 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 8941 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 9001 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 9061 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag 9121 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 9181 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 9241 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 9301 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 9361 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 9421 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc 9481 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 9541 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 9601 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 9661 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 9721 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 9781 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 9841 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 9901 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 9961 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 10021 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 10081 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 10141 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 10201 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 10261 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 10321 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 10381 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 10441 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 10501 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 10561 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 10621 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 10681 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 10741 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 10801 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 10861 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 10921 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 10981 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 11041 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 11101 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 11161 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 11221 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 11281 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 11341 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 11401 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 11461 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 11521 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc 11581 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 11641 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg 11701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 11761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 11821 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta 11881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc 11941 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc 12001 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt 12061 gtatttgtta aatatgtact acaaacttag tagt //