LOCUS Exported 10148 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of SMAD4 K436N. ACCESSION . VERSION . KEYWORDS pHAGE-SMAD4-K436N SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10148) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10148) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10148 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5108..5132) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5140..5158 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5183..5202) /label=T7 /note="T7 promoter, forward primer" promoter complement(5184..5202) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5208..5228) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5282..5323 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5344..5911 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5361..5913 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5505..5522) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5732..5751 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5925..6524 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5925..5944) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6421..6441 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6538..7126 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6591..6611) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7009..7020) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7201..7434 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7553..7571) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7639..7743 /gene="bla" /label=AmpR promoter CDS 7744..8604 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7962..7981) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8775..9363 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9264..9283 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9517..9534 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9626..9645 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9774..9795 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9810..9840 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9848..9864 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9853..9875 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9872..9888 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9872..9888 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9938..9957 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gacaatatgt ctattacgaa tacaccaaca 3481 agtaatgatg cctgtctgag cattgtgcat agtttgatgt gccatagaca aggtggagag 3541 agtgaaacat ttgcaaaaag agcaattgaa agtttggtaa agaagctgaa ggagaaaaaa 3601 gatgaattgg attctttaat aacagctata actacaaatg gagctcatcc tagtaaatgt 3661 gttaccatac agagaacatt ggatgggagg cttcaggtgg ctggtcggaa aggatttcct 3721 catgtgatct atgcccgtct ctggaggtgg cctgatcttc acaaaaatga actaaaacat 3781 gttaaatatt gtcagtatgc gtttgactta aaatgtgata gtgtctgtgt gaatccatat 3841 cactacgaac gagttgtatc acctggaatt gatctctcag gattaacact gcagagtaat 3901 gctccatcaa gtatgatggt gaaggatgaa tatgtgcatg actttgaggg acagccatcg 3961 ttgtccactg aaggacattc aattcaaacc atccagcatc caccaagtaa tcgtgcatcg 4021 acagagacat acagcacccc agctctgtta gccccatctg agtctaatgc taccagcact 4081 gccaactttc ccaacattcc tgtggcttcc acaagtcagc ctgccagtat actggggggc 4141 agccatagtg aaggactgtt gcagatagca tcagggcctc agccaggaca gcagcagaat 4201 ggatttactg gtcagccagc tacttaccat cataacagca ctaccacctg gactggaagt 4261 aggactgcac catacacacc taatttgcct caccaccaaa acggccatct tcagcaccac 4321 ccgcctatgc cgccccatcc cggacattac tggcctgttc acaatgagct tgcattccag 4381 cctcccattt ccaatcatcc tgctcctgag tattggtgtt ccattgctta ctttgaaatg 4441 gatgttcagg taggagagac atttaaggtt ccttcaagct gccctattgt tactgttgat 4501 ggatacgtgg acccttctgg aggagatcgc ttttgtttgg gtcaactctc caatgtccac 4561 aggacagaag ccattgagag agcaaggttg cacataggca aaggtgtgca gttggaatgt 4621 aaaggtgaag gtgatgtttg ggtcaggtgc cttagtgacc acgcggtctt tgtacagagt 4681 tactacttag acagagaagc tgggcgtgca cctggagatg ctgttcataa gatctaccca 4741 agtgcatata taaacgtctt tgatttgcgt cagtgtcatc gacagatgca gcagcaggcg 4801 gctactgcac aagctgcagc agctgcccag gcagcagccg tggcaggaaa catccctggc 4861 ccaggatcag taggtggaat agctccagct atcagtctgt cagctgctgc tggaattggt 4921 gttgatgacc ttcgtcgctt atgcatactc aggatgagtt ttgtgaaagg ctggggaccg 4981 gattacccaa gacagagcat caaagaaaca ccttgctgga ttgaaattca cttacaccgg 5041 gccctccagc tcctagacga agtacttcat accatgccga ttgcagaccc acaaccttta 5101 gactagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc actaaaggta 5161 ttatgagaaa gttgaatagt agccctatag tgagtcgtat taccacccaa cttttctata 5221 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga 5281 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg 5341 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc 5401 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa 5461 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat 5521 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac 5581 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg 5641 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt 5701 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg 5761 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac 5821 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga 5881 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc gagtacaagc 5941 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 6001 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 6061 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 6121 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 6181 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 6241 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 6301 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 6361 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 6421 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 6481 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat agatcctaat 6541 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6601 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6661 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6721 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6781 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6841 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6901 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 6961 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7021 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 7081 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa 7141 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 7201 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 7261 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 7321 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7381 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 7441 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7501 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg 7561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 7621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 7681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 7741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 7801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 7861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 7921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 7981 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 8041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 8101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 8161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 8221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 8281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 8341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 8401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 8461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 8521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 8581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 8641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 8701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 8761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 8821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 8881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 8941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 9001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 9061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 9121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 9181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 9241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 9301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 9361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 9421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 9481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 9541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 9601 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 9661 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 9721 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 9781 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 9841 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat 9901 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9961 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 10021 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 10081 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 10141 ttagtagt //