LOCUS Exported 13375 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of ALK R1275Q. ACCESSION . VERSION . KEYWORDS pHAGE-ALK-R1275Q SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13375) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 13375) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13375 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 20..44 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4911..4935) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4988..5029 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5050..5617 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 5067..5619 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5211..5228) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5438..5457 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5631..6350 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 5631..6347 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5676..5697) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5937..5956) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6284..6305 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6364..6952 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6417..6437) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6835..6846) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7027..7260 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7379..7397) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7465..7569 /gene="bla" /label=AmpR promoter CDS 7570..8430 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7788..7807) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8601..9189 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9090..9109 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9343..9360 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9452..9471 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9600..9621 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9636..9666 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9674..9690 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9679..9701 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9698..9714 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9698..9714 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9764..9783 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 9975..10608 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10655..10780 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11277..11510 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11695..11739 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 12032..12149 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 12197..13375 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 12428..13366 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 13323..13343 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg gagccatcgg 61 gctcctgtgg ctcctgccgc tgctgctttc cacggcagct gtgggctccg ggatggggac 121 cggccagcgc gcgggctccc cagctgcggg gccgccgctg cagccccggg agccactcag 181 ctactcgcgc ctgcagagga agagtctggc agttgacttc gtggtgccct cgctcttccg 241 tgtctacgcc cgggacctac tgctgccacc atcctcctcg gagctgaagg ctggcaggcc 301 cgaggcccgc ggctcgctag ctctggactg cgccccgctg ctcaggttgc tggggccggc 361 gccgggggtc tcctggaccg ccggttcacc agccccggca gaggcccgga cgctgtccag 421 ggtgctgaag ggcggctccg tgcgcaagct ccggcgtgcc aagcagttgg tgctggagct 481 gggcgaggag gcgatcttgg agggttgcgt cgggcccccc ggggaggcgg ctgtggggct 541 gctccagttc aatctcagcg agctgttcag ttggtggatt cgccaaggcg aagggcgact 601 gaggatccgc ctgatgcccg agaagaaggc gtcggaagtg ggcagagagg gaaggctgtc 661 cgcggcaatt cgcgcctccc agccccgcct tctcttccag atcttcggga ctggtcatag 721 ctccttggaa tcaccaacaa acatgccttc tccttctcct gattatttta catggaatct 781 cacctggata atgaaagact ccttcccttt cctgtctcat cgcagccgat atggtctgga 841 gtgcagcttt gacttcccct gtgagctgga gtattcccct ccactgcatg acctcaggaa 901 ccagagctgg tcctggcgcc gcatcccctc cgaggaggcc tcccagatgg acttgctgga 961 tgggcctggg gcagagcgtt ctaaggagat gcccagaggc tcctttctcc ttctcaacac 1021 ctcagctgac tccaagcaca ccatcctgag tccgtggatg aggagcagca gtgagcactg 1081 cacactggcc gtctcggtgc acaggcacct gcagccctct ggaaggtaca ttgcccagct 1141 gctgccccac aacgaggctg caagagagat cctcctgatg cccactccag ggaagcatgg 1201 ttggacagtg ctccagggaa gaatcgggcg tccagacaac ccatttcgag tggccctgga 1261 atacatctcc agtggaaacc gcagcttgtc tgcagtggac ttctttgccc tgaagaactg 1321 cagtgaagga acatccccag gctccaagat ggccctgcag agctccttca cttgttggaa 1381 tgggacagtc ctccagcttg ggcaggcctg tgacttccac caggactgtg cccagggaga 1441 agatgagagc cagatgtgcc ggaaactgcc tgtgggtttt tactgcaact ttgaagatgg 1501 cttctgtggc tggacccaag gcacactgtc accccacact cctcaatggc aggtcaggac 1561 cctaaaggat gcccggttcc aggaccacca agaccatgct ctattgctca gtaccactga 1621 tgtccccgct tctgaaagtg ctacagtgac cagtgctacg tttcctgcac cgatcaagag 1681 ctctccatgt gagctccgaa tgtcctggct cattcgtgga gtcttgaggg gaaacgtgtc 1741 cttggtgcta gtggagaaca aaaccgggaa ggagcaaggc aggatggtct ggcatgtcgc 1801 cgcctatgaa ggcttgagcc tgtggcagtg gatggtgttg cctctcctcg atgtgtctga 1861 caggttctgg ctgcagatgg tcgcatggtg gggacaagga tccagagcca tcgtggcttt 1921 tgacaatatc tccatcagcc tggactgcta cctcaccatt agcggagagg acaagatcct 1981 gcagaataca gcacccaaat caagaaacct gtttgagaga aacccaaaca aggagctgaa 2041 acccggggaa aattcaccaa gacagacccc catctttgac cctacagttc attggctgtt 2101 caccacatgt ggggccagcg ggccccatgg ccccacccag gcacagtgca acaacgccta 2161 ccagaactcc aacctgagcg tggaggtggg gagcgagggc cccctgaaag gcatccagat 2221 ctggaaggtg ccagccaccg acacctacag catctcgggc tacggagctg ctggcgggaa 2281 aggcgggaag aacaccatga tgcggtccca cggcgtgtct gtgctgggca tcttcaacct 2341 ggagaaggat gacatgctgt acatcctggt tgggcagcag ggagaggacg cctgccccag 2401 tacaaaccag ttaatccaga aagtctgcat tggagagaac aatgtgatag aagaagaaat 2461 ccgtgtgaac agaagcgtgc atgagtgggc aggaggcgga ggaggagggg gtggagccac 2521 ctacgtattt aagatgaagg atggagtgcc ggtgcccctg atcattgcag ccggaggtgg 2581 tggcagggcc tacggggcca agacagacac gttccaccca gagagactgg agaataactc 2641 ctcggttcta gggctaaacg gcaattccgg agccgcaggt ggtggaggtg gctggaatga 2701 taacacttcc ttgctctggg ccggaaaatc tttgcaggag ggtgccaccg gaggacattc 2761 ctgcccccag gccatgaaga agtgggggtg ggagacaaga gggggtttcg gagggggtgg 2821 aggggggtgc tcctcaggtg gaggaggcgg aggatatata ggcggcaatg cagcctcaaa 2881 caatgacccc gaaatggatg gggaagatgg ggtttccttc atcagtccac tgggcatcct 2941 gtacacccca gctttaaaag tgatggaagg ccacggggaa gtgaatatta agcattatct 3001 aaactgcagt cactgtgagg tagacgaatg tcacatggac cctgaaagcc acaaggtcat 3061 ctgcttctgt gaccacggga cggtgctggc tgaggatggc gtctcctgca ttgtgtcacc 3121 caccccggag ccacacctgc cactctcgct gatcctctct gtggtgacct ctgccctcgt 3181 ggccgccctg gtcctggctt tctccggcat catgattgtg taccgccgga agcaccagga 3241 gctgcaagcc atgcagatgg agctgcagag ccctgagtac aagctgagca agctccgcac 3301 ctcgaccatc atgaccgact acaaccccaa ctactgcttt gctggcaaga cctcctccat 3361 cagtgacctg aaggaggtgc cgcggaaaaa catcaccctc attcggggtc tgggccatgg 3421 cgcctttggg gaggtgtatg aaggccaggt gtccggaatg cccaacgacc caagccccct 3481 gcaagtggct gtgaagacgc tgcctgaagt gtgctctgaa caggacgaac tggatttcct 3541 catggaagcc ctgatcatca gcaaattcaa ccaccagaac attgttcgct gcattggggt 3601 gagcctgcaa tccctgcccc ggttcatcct gctggagctc atggcggggg gagacctcaa 3661 gtccttcctc cgagagaccc gccctcgccc gagccagccc tcctccctgg ccatgctgga 3721 ccttctgcac gtggctcggg acattgcctg tggctgtcag tatttggagg aaaaccactt 3781 catccaccga gacattgctg ccagaaactg cctcttgacc tgtccaggcc ctggaagagt 3841 ggccaagatt ggagacttcg ggatggccca agacatctac agggcgagct actatagaaa 3901 gggaggctgt gccatgctgc cagttaagtg gatgccccca gaggccttca tggaaggaat 3961 attcacttct aaaacagaca catggtcctt tggagtgctg ctatgggaaa tcttttctct 4021 tggatatatg ccatacccca gcaaaagcaa ccaggaagtt ctggagtttg tcaccagtgg 4081 aggccggatg gacccaccca agaactgccc tgggcctgta taccggataa tgactcagtg 4141 ctggcaacat cagcctgaag acaggcccaa ctttgccatc attttggaga ggattgaata 4201 ctgcacccag gacccggatg taatcaacac cgctttgccg atagaatatg gtccacttgt 4261 ggaagaggaa gagaaagtgc ctgtgaggcc caaggaccct gagggggttc ctcctctcct 4321 ggtctctcaa caggcaaaac gggaggagga gcgcagccca gctgccccac cacctctgcc 4381 taccacctcc tctggcaagg ctgcaaagaa acccacagct gcagagatct ctgttcgagt 4441 ccctagaggg ccggccgtgg aagggggaca cgtgaatatg gcattctctc agtccaaccc 4501 tccttcggag ttgcacaagg tccacggatc cagaaacaag cccaccagct tgtggaaccc 4561 aacgtacggc tcctggttta cagagaaacc caccaaaaag aataatccta tagcaaagaa 4621 ggagccacac gacaggggta acctggggct ggagggaagc tgtactgtcc cacctaacgt 4681 tgcaactggg agacttccgg gggcctcact gctcctagag ccctcttcgc tgactgccaa 4741 tatgaaggag gtacctctgt tcaggctacg tcacttccct tgtgggaatg tcaattacgg 4801 ctaccagcaa cagggcttgc ccttagaagc cgctactgcc cctggagctg gtcattacga 4861 ggataccatt ctgaaaagca agaatagcat gaaccagcct gggccctaga acccagcttt 4921 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg 4981 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta 5041 gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 5101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 5161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 5221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 5281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 5341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 5401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 5461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 5521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca 5581 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca 5641 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 5701 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 5761 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 5821 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 5881 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 5941 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 6001 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 6061 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 6121 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 6181 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 6241 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 6301 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat 6361 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc 6961 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7021 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 7081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7261 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 7321 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc 7381 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 7441 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 7501 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 7561 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 7621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 7681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 7741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 7801 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 7861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 7921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 7981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 8041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 8101 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 8161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 8221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 8281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 8341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 8401 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 8461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 8521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 8581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 8641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 8701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 8761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 8821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 8881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 8941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 9001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 9061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 9121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 9181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 9241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 9301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 9361 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 9421 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 9481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 9541 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 9601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 9661 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt 9721 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9781 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 9841 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 9901 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 9961 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct 10021 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg 10081 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa 10141 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg 10201 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca 10261 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta 10321 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg 10381 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt 10441 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 10501 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 10561 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 10621 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 10681 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 10741 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 10801 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 10861 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 10921 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 10981 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 11041 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 11101 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg 11161 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 11221 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 11281 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 11341 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 11401 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 11461 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 11521 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 11581 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 11641 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 11701 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 11761 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 11821 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 11881 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 11941 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc 12001 cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt 12061 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac 12121 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag 12181 tttggactag tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 12241 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg 12301 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 12361 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 12421 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc 12481 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg 12541 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc 12601 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg 12661 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc 12721 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat 12781 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc 12841 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg 12901 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct 12961 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg 13021 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag 13081 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc 13141 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga 13201 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc 13261 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat 13321 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtga //